Regulation of spermatogenesis and reproductive capacity by Igf3 in tilapia

Li, Minghui; Liu, Xingyong; Dai, Shengfei; Xiao, Hesheng; Qi, Shuangshuang; Li, Yibing; Zheng, Qiaoyuan; Jie, Mimi; Cheng, Christopher H.K.; Wang, Deshou

doi:10.1007/s00018-019-03439-0

Cited by 35 publications

(23 citation statements)

References 82 publications

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“…Reproduction is under multifactorial control of neurohormones, pituitary hormones, glucocorticoids, growth factors, thyroid hormones as well as a number of gonadal hormones including steroids and peptides (Schulz et al, 2010; Tovo-Neto et al, 2018; Fallah 2019, 2020, 2021; Ma et al, 2020a, b; Tovo-Neto et al, 2020; Li et al, 2020). A number of neurohormones including Gnih (Tsutsui et al, 2000) were shown to be involved in the control of pituitary gonadotropin production (Amano et al, 2006; Moussavi et al, 2012, 2013, 2014; Choi et al, 2016; Paullada-Salmerón et al, 2016; Muñoz-Cueto et al, 2017; Branco et., 2018; Ma et al, 2020 a, b) as well as being involved in paracrine control of gonadal function in fish (Fallah et al, 2019, 2020, 2021).…”

Section: Discussionmentioning

confidence: 99%

Interaction of thyroid hormones and gonadotropin inhibitory hormone in the multifactorial control of zebrafish (Danio rerio) spermatogenesis

Rodrigues

Fallah

Zanardini

et al. 2021

Preprint

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Reproduction is under multifactorial control of neurohormones, pituitary gonadotropins, as well as a number of gonadal hormones including sex steroids and growth factors. Gonadotropin-inhibitory hormone (Gnih), a novel RFamide neuropeptide, was shown to be involved in the control of pituitary gonadotropin production, as well as being involved as a paracrine factor in the regulation of gonadal function. In this context, recent studies have demonstrated that Gnih inhibited gonadotropin-induced spermatogenesis in the zebrafish testicular explants. Thyroid hormones are known to interact with the reproductive axis, and are, in particular, involved in the regulation of testicular function. Based on this background, we investigated the interaction between Gnih and thyroid hormones in the control of zebrafish spermatogenesis. To this end, zebrafish adult males were treated with the goitrogen methimazole (1mM for 21 days) in order to generate a hypothyroid model organism. Subsequently, a factorial design using an ex vivo testis culture system in combination with histomorphometrical and FACScan cell cycle analyses were adopted. Our results showed that methimazole treatment affected both basal and gonadotropin-induced spermatogenesis, in particular, meiosis and spermiogenesis. Moreover, the goitrogen treatment nullified the inhibitory actions of Gnih on the gonadotropin-induced spermatogenesis, specifically in the haploid cell population. We have demonstrated that thyroid hormones interaction with gonadotropin and Gnih are important components for the regulation of zebrafish spermatogenesis. The results provide a support for the hypothesis that thyroid hormones are important contributors in multifactorial control of spermatogenesis in zebrafish.

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Section: Discussionmentioning

confidence: 99%

Interaction of thyroid hormones and gonadotropin inhibitory hormone in the multifactorial control of zebrafish (Danio rerio) spermatogenesis

Rodrigues

Fallah

Zanardini

et al. 2021

Preprint

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“…Of the articles retrieved in this review, several reported mosaicisms in their research animals. 12,47,49,51,53,57,59,61,71 Straume et al 62 reported that mosaicism increased with higher…”

Section: Mosaicismmentioning

confidence: 99%

Genome editing on finfish: Current status and implications for sustainability

Blix

Dalmo²,

Wargelius

et al. 2021

Reviews in Aquaculture

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Aquaculture is the fastest growing food production industry on a world basis. In 2018, the share of aquaculture in total fish production was 46%. 1 Even though the production is growing, the negative effects of this industry often receive much attention. These challenges include diseases, escapees and ecological effects. 2 In Norway, the first-hand value of Atlantic salmon was 68 billion NOK in 2019, 3 and

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“…The dilution and specificity of these antibodies have been analyzed previously [76][77][78]. For IF, Alexa Fluor 488-and 594-conjugated secondary antibodies (Invitrogen, Shanghai, China) were diluted 1:500 in blocking solution and incubated with tissue to detect the primary antibodies.…”

Section: Immunofluorescence (If) Tunel Assay and Immunohistochemistry (Ihc)mentioning

confidence: 99%

Dnmt3aa But Not Dnmt3ab Is Required for Maintenance of Gametogenesis in Nile Tilapia

Wang

Qin

et al. 2021

Preprint

Self Cite

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Background: Dnmt3a , a de novo methylatransferase, is essential for both male and female germ line DNA methylation. Only one Dnmt3a is identified in mammals, and homozygous mutation of Dnmt3a is lethal, while two Dnmt3a , dnmt3aa and dnmt3ab , are identified in teleosts due to the third round of genome duplication, and homozygous mutation of dnmt3aa and dnmt3ab is viable in zebrafish. Dnmt3aa and dnmt3ab were demonstrated to have essential and non-overlapped functions on modulating behavioral control, however, their function in gonadal development is unclear in fish. Results: In this study, the expression patterns of dnmt3aa and dnmt3ab in developing gonads of Nile tilapia was analyzed by quantitative real time PCR and fluorescence in situ hybridization. Both dnmt3aa and dnmt3ab displayed sexually dimorphic expression in developing gonads. Dnmt3aa was widely expressed in gonadal germ cells and somatic cells, highly expressed in oogonia, phase I and II oocytes and granulosa cells in ovaries and spermatogonia and spermatocytes in testes, while dnmt3ab was mainly expressed in ovarian granulosa cells and testicular spermatocytes. Mutation of dnmt3aa and dnmt3ab was achieved by CRISPR/Cas9 in tilapia. Lower GSI (Gonadosomatic index), increased apoptosis of oocytes and spermatocytes and significantly reduced sperm quality were observed in dnmt3aa −/− mutants, while no obvious phenotype was observed in dnmt3ab −/− mutants. Consistently, the expression of apoptotic genes was significantly increased in dnmt3aa −/− mutants. In addition, dnmt3aa and dnmt3ab were found to have certain compensatory effects in the gonads. The global DNA methylation level in ovaries and testes of dnmt3aa −/− mutants was decreased significantly, compared with that of dnmt3ab −/− mutants and WT. Conclusions: Taken together, our results suggest that dnmt3aa , not dnmt3ab , plays important roles in maintaining gametogenesis in teleost. Our results enrich the understanding of the function of DNA methyltransferases in gonads of non-mammalian vertebrates.

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Regulation of spermatogenesis and reproductive capacity by Igf3 in tilapia

Cited by 35 publications

References 82 publications

Interaction of thyroid hormones and gonadotropin inhibitory hormone in the multifactorial control of zebrafish (Danio rerio) spermatogenesis

Interaction of thyroid hormones and gonadotropin inhibitory hormone in the multifactorial control of zebrafish (Danio rerio) spermatogenesis

Genome editing on finfish: Current status and implications for sustainability

Dnmt3aa But Not Dnmt3ab Is Required for Maintenance of Gametogenesis in Nile Tilapia

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