Regulation of Soybean Embryogenesis by Abscisic Acid

Ackerson, Robert C.

doi:10.1093/jxb/35.3.403

Cited by 106 publications

(50 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is in accordance with soybean zygotic embryo development, in that cotyledons of zygotic embryos did not grow unless the growth media were supplemented with 1 or 10 µM ABA (Ackerson 1984). In another study of soybean somatic embryo induction directly from cotyledon explants, Lazzeri et al (1987) reported that ABA at low concentration (0.38 µM) slightly increased the frequency of normal somatic embryos over control (no data were provided).…”

Section: Resultssupporting

confidence: 63%

“…1a). ABA has been observed to stimulate the growth of soybean zygotic embryos cultured in vitro during the early phase of embryogenesis (Ackerson 1984). Quebedeaux et al (1976) also reported that, in developing seeds of soybean, the endogenous ABA level correlated with growth rate, being the highest during the most active growth period of seed enlargement.…”

Section: Resultsmentioning

confidence: 97%

“…Maturation of somatic embryos is a process during which a large amount of nutrients accumulate (Buchheim et al 1989). ABA has been reported to stimulate protein accumulation of soybean zygotic embryos cultured in vitro during the early phase of embryogenesis (Ackerson 1984).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Improvement of soybean somatic embryo development and maturation by abscisic acid treatment

Tian¹,

Brown²

2000

Can. J. Plant Sci.

View full text Add to dashboard Cite

Improvement of soybean somatic embryo development and maturation by abscisic acid treatment. Can. J. Plant Sci. 80: 271-276. Recovery of tissue culture-derived plants through somatic embryogenesis is a useful system for genetic engineering of soybean. The effect of abscisic acid (ABA) on soybean somatic embryogenesis, development,and maturation was investigated. ABA at 1, 10, 50, 100, and 500 µM were applied at different stages of embryo development; namely, at the globular stage in suspension culture, at the development stage and at the maturation stage on solid media. ABA promoted embryo growth and development when applied at the globular stage. Embryo size, after 15 d and after 1 mo on development medium, was significantly greater than that without exposure to ABA. ABA promoted normal embryo morphogenesis and 62% more normal embryos developed when embryos were treated with ABA at the globular stage. ABA treated-embryos showed an increased tolerance to partial desiccation (from 24% to 78%) and exhibited an increased germination capability relative to non-ABA-treated controls (54% versus 8%). Somatic embryos appeared to undergo a decreasing sensitivity to ABA during maturation. ABA did not show an effect when applied during embryo development and maturation stages. A protocol for more normal embryo formation and improved embryo germination is reported.

show abstract

Section: Resultssupporting

confidence: 63%

Section: Resultsmentioning

confidence: 97%

See 1 more Smart Citation

Improvement of soybean somatic embryo development and maturation by abscisic acid treatment

Tian¹,

Brown²

2000

Can. J. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Phloem-mobile elements such as K+ and Mg2+ mostly follow the path of C and N, being retranslocated from stem and leaf tissue to the developing embryo (Pitman, 1975;Mauk and Noodén, 1992). The mobilization of these ions is subject to hormonal signals (Neuman and Noodén, 1983;Noodén, 1987;Mauk and Noodén, 1992), as are C and N nutrients (Ackerson, 1984;Guldan and Brun, 1987;Schussler et al, 1991). Phloem-immobile ions such as Ca2+ must take a different route.…”

mentioning

confidence: 99%

Changes in Soybean Fruit Ca2+ (Sr2+) and K+ (Rb+) Transport Ability during Development

Laszlo

1994

Plant Physiol.

View full text Add to dashboard Cite

Mineral uptake by soybean (Glycine max [L.] Merrill) seeds during development can significantly affect seed quality and value. Little is known about seed mineral transport mechanisms and control processes, although it is clear that each mineral displays a characteristic accumulation pattern. lon-specific accumulation patterns could result from changes in source availability, in transport kinetics through the seed pod and seed coat, or in the mineral uptake capability of the embryo. CaZ+ and K+ have negligible and high phloem mobilities, respectively. CaZ+ accumulation lags behind dry matter (C and N) and K+ accumulation in soybean embryos. To eliminate source availability influences, the CaZ+ and K+ uptake ability of isolated embryos and of seeds in pod culture was examined during seed development. SrZ+ and Rb+ were used as transport analogs of Ca*+ and K+, respectively. SrZ+ and Rb+ uptake rates by isolated embryos increased with seed fresh weight, indicating that the embryo was not limiting ca*+ accumulation. However, the pod-cultured embryo Sr2+ and Rb+ uptake rate trends differed: Rb+ uptake increased with seed fresh weight, whereas SrZ+ uptake rates remained constant or decreased slightly. Ovule SrZ+ influx data suggest that the pod and seed coat impose a transport barrier that could account for the relative decline in embryo CaZ+ content during development.

show abstract

“…Correlations between rate of growth and ABA accumulation in major sinks have been reported for wheat (13,14), grapes (5), pea (3), bean (10), and soybeans (4, 15). The relatively high levels (up to 12.2 #g g-' fresh weight) (15) and timing of ABA accumulation in soybean seeds (1,4,15) suggest a role in seed growth above simply preventing precocious germination. Browning (3) found that ABA accumulated more rapidly in a lare-seeded pea line and suggested a role for ABA in controlling cotyledon enlargement during linear seed fill.…”

mentioning

confidence: 99%

Abscisic Acid and Its Relationship to Seed Filling in Soybeans

Schussler¹,

Brenner²,

Brun³

1984

Plant Physiol.

134

View full text Add to dashboard Cite

ABSTRACrThe effect of exogenous abscisic acid (ABA) on the rate of sucrose uptake by soybean (Glycine max L. Men.) embryos was evaluated in an in vitro system. In addition, the concentrations of endogenous ABA in seeds of three soybean Plant Introduction (PI) lines, differing in seed size, were commpared to their seed growth rates. ABA (10-molar) stimulated in vitro sucrose uptake in soybean (cv 'Cly') embryos removed from plants grown in a controled environment chamber, but not in embryos removed from field-grown plants of the Sink strength describes the capacity ofan organ to accumulate photoassimilates, and is the product of sink size and sink activity (25). Plant hormones are probably involved in determining both sink size and sink intensity. While cytokinins, gibberellins, and auxins generally promote growth processes, ABA has most often been considered an inhibitory substance (24). However, promotive effects of ABA in sink tissues have now been reported. ABA enhanced sugar content in roots of intact bean plants, possibly by stimulating sugar transport from the shoot to the root (11). In some cases, applications of ABA to ears of wheat (7) and barley (22) have stimulated 14C assimilate transfer from the flag leaf to the ear. However, King and Patrick (12) saw no stimulation of 14C transport to wheat ears after injection of ABA into the grains. Correlations between rate of growth and ABA accumulation in major sinks have been reported for wheat (13,14), grapes (5), pea (3), bean (10), and soybeans (4, 15). The relatively high levels (up to 12.2 #g g-' fresh weight) (15) and timing of ABA accumulation in soybean seeds (1,4,15) suggest a role in seed growth above simply preventing precocious germination. Browning (3) found that ABA accumulated more rapidly in a lare-seeded pea line and suggested a role for ABA in controlling cotyledon enlargement during linear seed fill. It has also been suggested that ABA may stimulate phloem unloading (8, 18) by decreasing the proton motive force across the sieve tube plasmalemma. The embryo-seed coat interface of the soybean seed (21) In addition, the endogenous ABA concentrations of seed coats, cotyledons, and embryonic axes of three field-grown soybean PI lines differing in seed size were compared to their seed growth rates during filling. MATERIALS AND METHODSGrowth Chamber Study. Plant Culture. Variety 'Clay' soybeans (Group 00 maturity) were grown in a controlled environment chamber in 1-L plastic pots in a 1.5:1.5:1 (v/v/v) mixture of vermiculite, peat, and sand. Pots were watered twice daily with automatic drip irrigation and given 250 ml of Peters 20-20-20 (200 ppm) twice weekly. A 14-h photoperiod with a 25/19C day/night temperature regime was provided. The photosynthetic photon flux density at the top of the canopy was 800 jE m-2 s-1.In Vitro Sucrose Uptake. During mid pod fill (seed dry weight 40% of final seed dry weight) pods were removed. Seed coats were surgically removed from a number of seeds and groups of three embryos were placed in 15 ml of sterile 10 ...

show abstract

Regulation of Soybean Embryogenesis by Abscisic Acid

Cited by 106 publications

References 23 publications

Improvement of soybean somatic embryo development and maturation by abscisic acid treatment

Improvement of soybean somatic embryo development and maturation by abscisic acid treatment

Changes in Soybean Fruit Ca2+ (Sr2+) and K+ (Rb+) Transport Ability during Development

Abscisic Acid and Its Relationship to Seed Filling in Soybeans

Contact Info

Product

Resources

About