Regulation of Secondary Metabolism in the Gray Mold Fungus<i>Botrytis cinerea</i>

Viaud, Muriel; Schumacher, Julia; Porquier, Antoine; Simon, Adeline

doi:10.1002/9783527682386.ch11

Cited by 8 publications

(4 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In A. flavus , NsdD affects morphogenesis and aflatoxin biosynthesis ( Cary et al, 2012 ). Deletion of LTF1 in B. cinerea affects the expression of known (e.g., for botcinic acid, botrydial and DHN melanin) and yet uncharacterized SM biosynthetic genes ( Schumacher et al, 2014 ; Viaud et al, 2016 ). These data support the general observation that major regulators of differentiation, such as orthologs of A. nidulans VeA, LaeA, MtfA, and NsdD are also regulators of secondary metabolism ( Bayram and Braus, 2012 ; Cary et al, 2015 ; Lind et al, 2015 , 2016 ; Schumacher et al, 2015 ; Zhuang et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

The GATA-Type Transcription Factor Csm1 Regulates Conidiation and Secondary Metabolism in Fusarium fujikuroi

et al. 2017

Self Cite

View full text Add to dashboard Cite

GATA-type transcription factors (TFs) such as the nitrogen regulators AreA and AreB, or the light-responsive TFs WC-1 and WC-2, play global roles in fungal growth and development. The conserved GATA TF NsdD is known as an activator of sexual development and key repressor of conidiation in Aspergillus nidulans, and as light-regulated repressor of macroconidia formation in Botrytis cinerea. In the present study, we functionally characterized the NsdD ortholog in Fusarium fujikuroi, named Csm1. Deletion of this gene resulted in elevated microconidia formation in the wild-type (WT) and restoration of conidiation in the non-sporulating velvet mutant Δvel1 demonstrating that Csm1 also plays a role as repressor of conidiation in F. fujikuroi. Furthermore, biosynthesis of the PKS-derived red pigments, bikaverin and fusarubins, is de-regulated under otherwise repressing conditions. Cross-species complementation of the Δcsm1 mutant with the B. cinerea ortholog LTF1 led to full restoration of WT-like growth, conidiation and pigment formation. In contrast, the F. fujikuroi CSM1 rescued only the defects in growth, the tolerance to H2O2 and virulence, but did not restore the light-dependent differentiation when expressed in the B. cinerea Δltf1 mutant. Microarray analysis comparing the expression profiles of the F. fujikuroi WT and the Δcsm1 mutant under different nitrogen conditions revealed a strong impact of this GATA TF on 19 of the 47 gene clusters in the genome of F. fujikuroi. One of the up-regulated silent gene clusters is the one containing the sesquiterpene cyclase-encoding key gene STC1. Heterologous expression of STC1 in Escherichia coli enabled us to identify the product as the volatile bioactive compound (–)-germacrene D.

show abstract

Section: Discussionmentioning

confidence: 99%

The GATA-Type Transcription Factor Csm1 Regulates Conidiation and Secondary Metabolism in Fusarium fujikuroi

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…To determine if BcBot6 might have a role in the regulation of other SM gene clusters, we performed, as above, expression assays using qRT-PCR on genes belonging to different SM gene clusters that were also shown to be expressed during infection and to be regulated through the Velvet complex (reviewed in Viaud et al, 2016). The first gene tested was Bcboa6 (Bcin01g00060) that encodes a PKS involved in the production of BOA (Dalmais et al, 2011) and whose expression is positively regulated by both BcVel1 and BcLae1 (Schumacher et al, 2015).…”

Section: Bcbot6 Regulates the Bcbot Genesmentioning

confidence: 99%

The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6

Porquier¹,

Morgant²,

Moraga³

et al. 2016

Fungal Genetics and Biology

Self Cite

View full text Add to dashboard Cite

a b s t r a c tBotrydial (BOT) is a non-host specific phytotoxin produced by the polyphagous phytopathogenic fungus Botrytis cinerea. The genomic region of the BOT biosynthetic gene cluster was investigated and revealed two additional genes named Bcbot6 and Bcbot7. Analysis revealed that the G + C/A + T-equilibrated regions that contain the Bcbot genes alternate with A + T-rich regions made of relics of transposable elements that have undergone repeat-induced point mutations (RIP). Furthermore, BcBot6, a Zn(II) 2 Cys 6 putative transcription factor was identified as a nuclear protein and the major positive regulator of BOT biosynthesis. In addition, the phenotype of the DBcbot6 mutant indicated that BcBot6 and therefore BOT are dispensable for the development, pathogenicity and response to abiotic stresses in the B. cinerea strain B05.10. Finally, our data revealed that B. pseudocinerea, that is also polyphagous and lives in sympatry with B. cinerea, lacks the ability to produce BOT. Identification of BcBot6 as the major regulator of BOT synthesis is the first step towards a comprehensive understanding of the complete regulation network of BOT synthesis and of its ecological role in the B. cinerea life cycle.

show abstract

“…In addition, the observed changes in secondary metabolism proteins, notably those involved in botrytdial and botcinic acid biosynthesis corroborate interaction of Sak1 with the G-protein, cAMP and Ca 2+ signaling. Indeed, all these pathways were shown to be involved in the regulation of transcription of BOT and BOA gene clusters and production of these SM (reviewed in [82]). The finding that OAH production is under Sak1 control is interesting as well.…”

Section: Link To Other Signaling Pathwaysmentioning

confidence: 99%

“…Protein enrichment in functional categories according to expert annotation[6,40,48,49,82] of detected proteins according to regulatory clusters. The red bar indicates significantly enriched functional categories, the length of the bars reflects the proportion of enriched proteins relative to the reference genome[40,52].…”

mentioning

confidence: 99%

Comparative quantitative proteomics of osmotic signal transduction mutants in Botrytis cinerea explain mutant phenotypes and highlight interaction with cAMP and Ca2+ signalling pathways

Kilani

Davanture

Simon

et al. 2020

Journal of Proteomics

Self Cite

View full text Add to dashboard Cite

(SPS) for financial support of proteomics equipment. They thank Adeline Simon for helping with enrichment studies. AUTHOR CONTRIBUTIONS STATEMENT JK designed and performed all experiments, assisted by MD for the proteomics workflow. MZ performed proteomic data analysis and corresponding statistics. AS performed in silico cluster analysis and functional enrichment studies. SF and MZ conceived the initial project.

show abstract

Regulation of Secondary Metabolism in the Gray Mold FungusBotrytis cinerea

Cited by 8 publications

References 73 publications

The GATA-Type Transcription Factor Csm1 Regulates Conidiation and Secondary Metabolism in Fusarium fujikuroi

The GATA-Type Transcription Factor Csm1 Regulates Conidiation and Secondary Metabolism in Fusarium fujikuroi

The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6

Comparative quantitative proteomics of osmotic signal transduction mutants in Botrytis cinerea explain mutant phenotypes and highlight interaction with cAMP and Ca2+ signalling pathways

Contact Info

Product

Resources

About