Regulation of ABA dependent wound induced spreading cell death by MYB108

Abstract

SummaryWounding results in the controlled cell death of a few rows of cells adjacent to disrupted cells resulting in physical wound closure, which combined with phenolic compound deposition, prevents water loss and pathogen entry. The control of these processes remains uncharacterized.Cell death in a mutant of Arabidopsis thaliana lacking BOTRYTIS SENSITIVE1/MYB108 (BOS1/MYB108) function was characterized utilizing physiological, cell biological and genetic methods.The bos1 mutant has a wound induced runaway cell death that includes enhanced reactive oxygen species (ROS) production that followed the extent of enhanced cell death. Exogenous abscisic acid (ABA) enhanced wound induced cell death in Col-0 plants and was sufficient to trigger cell death in bos1. Uncontrolled cell death was dependent of the production and perception of ABA. Furthermore, bos1 had altered sensitivity to and accumulation of ABA.Arabidopsis possesses a genetic program controlling the extent of wound inducible cell death. BOS1 acts as a negative regulator of ABA induced cell death, which functions in the control of this wound sealing program. This program is distinct from other known cell death programs in that it is ABA dependent, but independent of salicylate biosynthesis, ethylene, jasmonate, metacaspases and ROS derived from RBOHD and RBOHF.

Roles of Defense Hormones in the Regulation of Ozone-Induced Changes in Gene Expression and Cell Death

“…Statistical analysis of the ion leakage percentage of O 3 -treated and clean air samples was performed by two-way ANOVA with the Tukey test using SigmaPlot 11.0 (P < 0.05). Visualization of H 2 O 2 in the leaf was performed with DAB staining on plants treated with O 3 for 4 h (Cui et al, 2013).…”

Cooperative Regulatory Functions of miR858 and MYB83 during Cyst Nematode Parasitism

“…7D), suggesting a role of MYB83 in forming a complex and highly interconnected regulatory network in the syncytium. Of the MYB transcription factors, MYB108, which regulates wound-induced cell death in an abscisic acid-dependent manner (Cui et al, 2013), and MYB51, a key regulator of indole glucosinolate biosynthesis (Frerigmann and Gigolashvili, 2014) were found. Additional MYB transcription factors included MYB12 and MYB59 that are involved in phenylpropanoid biosynthesis and cell cycle progression, respectively (Mehrtens et al, 2005;Mu et al, 2009).…”

Out for a Walk Along the Secretory Pathway During Programmed Cell Death

“…In line with the latter speculation, the TF identifi ed by Christiansen et al [ 138 ] are likely candidates to be phosphorylated by EDR1 and further ubiquitinated by KEG. More recently, knocking-out the nuclear TF Botrytis sensitive 1 (BOS1)/MYB108-encoding gene was found to provoke an ABA-contingent runaway cell death (RCD) in wounded Arabidopsis plants [ 142 ], unveiling a new regulatory mechanism whereby BOS1/MYB108 would limit cell death from spreading by diminishing ABA accumulation. Based on this study and the above analysis, it seems that the ABA signaling aspect that has long been neglected in the context of PCD may be of utmost relevance and therefore deserves further investigation in relation to the secretory pathway.…”