Regulation of Scaffold Cell Adhesion Using Artificial Membrane Binding Proteins

Burke, Madeline; Armstrong, James R.; Goodwin, Andrew E.; Deller, Robert C.; Carter, Benjamin M.; Harniman, Robert L.; Ginwalla, Aasiya; Ting, Valeska P.; Davis, Sean A.; Perriman, Adam W.

doi:10.1002/mabi.201600523

Cited by 14 publications

(10 citation statements)

References 41 publications

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“…Surface chemistry, topography, chemical composition, wettability, stiffness, dimensionality, porosity, and degree of cross‐linking are fundamental parameters that must be taken into the account in the design of substitute for medical applications . By manipulating surface properties of implants such as surface charge, functional groups, and topography, initial cell attachment can be tuned in a controlled manner …”

Section: Effects Of Surface Properties On Cellular Behaviorsmentioning

confidence: 99%

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Amani

Arzaghi

Bayandori

et al. 2019

Adv Materials Inter

305

200

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Surface interaction at the biomaterial–cell interface is essential for a variety of cellular functions, such as adhesion, proliferation, and differentiation. Nevertheless, changes in the biointerface enable to trigger specific cell signaling and result in different cellular responses. In order to manufacture biomaterials with higher functionality, biomaterials containing immobilized bioactive ligands have been widely introduced and employed for tissue engineering and regenerative medicine applications. Moreover, a number of physical and chemical strategies have been used to improve the functionality of biomaterials and specifically at the material interface. Here, the interactions between materials and cells at the interface levels are described. Then, the importance of surface properties in cell function is discussed and recent methods for surface modifications are systematically highlighted. Additionally, the impact of bulk material properties on the cellular responses is briefly reviewed.

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Section: Effects Of Surface Properties On Cellular Behaviorsmentioning

confidence: 99%

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Amani

Arzaghi

Bayandori

et al. 2019

Adv Materials Inter

305

200

View full text Add to dashboard Cite

show abstract

“…These include, a reduction in risk arising from oncogenesis in the therapeutic cells, minimal impact on the cell manufacturing process as the modification step can be readily integrated into an existing therapeutic pipeline, the display number per cell can be systematically varied to reduce the risks associated with patient-specific expression levels, and protein production is scalable and can be produced using good manufacturing practice procedures. 103,104 An excellent example of direct protein-based membrane modification was demonstrated by Won et al, who displayed recombinantly produced CXCR4 on MSC membranes using lipid-PEG vesicles (Figure 2A). 105 They demonstrated that CXCR4 was only present in the MSCs membrane after delivery by confocal microscopy studies without affecting the viability of MSCs and showed up to a twofold improvement in their migration toward SDF-1 following a concentration gradient in vitro.…”

Section: Protein-based Membrane Modificationsmentioning

confidence: 99%

“…Direct protein‐based membrane modification strategies provide transient display of the targeting construct and present a number of potential benefits over generic approaches for improved cell homing. These include, a reduction in risk arising from oncogenesis in the therapeutic cells, minimal impact on the cell manufacturing process as the modification step can be readily integrated into an existing therapeutic pipeline, the display number per cell can be systematically varied to reduce the risks associated with patient‐specific expression levels, and protein production is scalable and can be produced using good manufacturing practice procedures 103,104 …”

Section: Approaches For Improving Sc Homing and Retentionmentioning

confidence: 99%

Cell augmentation strategies for cardiac stem cell therapies

Cruz-Samperio

Jordan

Perriman

2021

Stem Cells Translational Medicine

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Myocardial infarction (MI) has been the primary cause of death in developed countries, resulting in a major psychological and financial burden for society. Current treatments for acute MI are directed toward rapid restoration of perfusion to limit damage to the myocardium, rather than promoting tissue regeneration and subsequent contractile function recovery. Regenerative cell therapies (CTs), in particular those using multipotent stem cells (SCs), are in the spotlight for treatment post-MI.Unfortunately, the efficacy of CTs is somewhat limited by their poor long-term viability, homing, and engraftment to the myocardium. In response, a range of novel SCbased technologies are in development to provide additional cellular modalities, bringing CTs a step closer to the clinic. In this review, the current landscape of emerging CTs and their augmentation strategies for the treatment post-MI are discussed. In doing so, we highlight recent advances in cell membrane reengineering via genetic modifications, recombinant protein immobilization, and the utilization of soft biomimetic scaffold interfaces.

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“…This can be related with the functionality changes induced by the EF at different intensities, namely the increase of the contact angle, swelling, porosity, degradation rate and elastic behaviour of the scaffolds. The changes in surface properties may facilitate the adhesion of the cells to the matrix, once electrostatic and hydrophobic contributions play a decisive role in such phenomenon [37,44]. Higher swelling and porosity also facilitate the proliferation and dispersion of the cells, as well as allow a better in and out-flow of nutrients, metabolites and gas exchanges [15,38].…”

Section: Cell Viability and Proliferationmentioning

confidence: 99%

Ohmic heating as a new tool for protein scaffold engineering

Rodrigues

Pereira

Vicente

et al. 2021

Materials Science and Engineering: C

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Ohmic heating (OH) is recognised as an emerging processing technology which recently is gaining increasing attention due to its ability to induce and control protein functionality. In this study, OH was used for the first time in the production of scaffolds for tissue engineering. BSA/casein solutions were processed by OH, promoting protein denaturation and aggregation, followed by cold-gelation through the addition of Ca 2+ . The formation of stable scaffolds was mostly dependent on the temperature and treatment time during OH processing. The variations of the electric field (EF) induced changes in the functional properties of both gel forming solutions and final scaffolds (contact angle, swelling, porosity, compressive modulus and degradation rate). The scaffolds' biological performance was evaluated regarding their ability to support the adhesion and proliferation of human fibroblast cells. The production process resulted in a non-cytotoxic material and the changes imposed by the presence of the EF during the scaffolds' production improved cellular proliferation and metabolic activity. Protein functionalization assisted by OH presents a promising new alternative for the production of improved and tuneable protein-based scaffolds for tissue engineering.

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Regulation of Scaffold Cell Adhesion Using Artificial Membrane Binding Proteins

Abstract: General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms FIGURE FOR ToC_ABSTRACT3

Cited by 14 publications

References 41 publications

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Controlling Cell Behavior through the Design of Biomaterial Surfaces: A Focus on Surface Modification Techniques

Cell augmentation strategies for cardiac stem cell therapies

Ohmic heating as a new tool for protein scaffold engineering

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