Regulation of Saccharomyces cerevisiae maltose fermentation by cold temperature and CSF1

Abstract

We studied the influence of cold temperature (10ºC) on the fermentation of maltose by a S. cerevisiae wild-type strain, and a csf1∆ mutant impaired in glucose and leucine uptake at low temperatures. Cold temperature affected the fermentation kinetics by decreasing the growth rate and the final cell yield, with almost no ethanol been produced from maltose by the wild-type cells at 10ºC. The csf1∆ strain did not grew on maltose when cultured at 10ºC, indicating that the CSF1 gene is also required for maltose consumption at low temperatures. However, this mutant also showed increased inhibition of glucose and maltose fermentation under salt stress, indicating that CSF1 is probably involved in the regulation of other physiological processes, including ion homeostasis.

Promotion of maltose fermentation at extremely low temperatures using a cryotolerant Saccharomyces cerevisiae strain immobilized on porous cellulosic material

“…Specifically, it has been demonstrated that expression of the MAL genes in S. cerevisiae is induced by maltose but repressed by glucose [6]. Works studying the regulation of maltose metabolism have also showed that maltose fermentation rate decreases at low temperatures, especially in cold sensitive yeasts [7]. On the other hand, low temperature fermentation (below 15 • C) has been recognized as a valuable tool to improve the quality of fermented products such as wine and beer.…”

Characterization of maltotriose transporters from theSaccharomyces eubayanussubgenome of the hybridSaccharomyces pastorianuslager brewing yeast strain Weihenstephan 34/70

“…The presence of mutant AGT1 genes in lager yeasts has been suggested to be correlated with the low temperatures at which this type of beer is made. While maltose fermentation by S. cerevisiae ale strains is highly dependent on temperature (these strains ferment poorly at temperatures below 12°C, see Hollatz and Stambuk ; Rautio and Londesborough ), lager yeasts perform well in low‐temperature wort fermentations. Indeed, Vidgren et al .…”

Βιοδιυλιστήριο Για Παραγωγή Μονοκυτταρικής Πρωτεΐνης, Εδώδιμων Μυκήτων Και Πρόσθετων Τροφίμων Με Βιοτεχνολογική Αξιοποίηση Μικτών Αγροτοβιομηχανικών Αποβλήτων

“…Η ερευνητική μελέτη του ρυθμού μεταβολισμού της μαλτόζης, έδειξαν ότι ο ρυθμός ζύμωσης της μαλτόζης μειώνεται σε χαμηλές τιμές θερμοκρασίας ειδικά σε κρυοευαίσθητες ζύμες (Hollatz & Stambuk, 2003 (Bardi & Koutinas, 1994;Iconomou et al, 1995;Koutinas et al, 2012 (Bardi & Koutinas, 1994 (Wang et al, 2002). Τόσο η πούλπα πορτοκαλιού όσο και η μελάσσα είναι απόβλητα πλούσια σε μέταλλα όπως μαγνήσιο, μαγγάνιο, σίδηρο και χαλκό αλλά και σε φωσφορικά άλατα που είναι απαραίτητα για την αύξηση των κυττάρων (Boulton et al, 1996;Αγγελόπουλος, 2013).…”