Regulation of ribosome biogenesis in maize embryonic axes during germination

Villa-Hernández, Juan Manuel; Dinkova, Tzvetanka D.; Aguilar-Caballero, R.; Rivera-Cabrera, Fernando; Jiménez, Estela Sánchez de; Pérez‐Flores, Laura J.

doi:10.1016/j.biochi.2013.06.011

Cited by 12 publications

(8 citation statements)

References 60 publications

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“…The following temperature program was used: reverse transcription reaction at 37°C for 10 min, initial denaturation at 95°C for 3 min, 94°C for 30 s, annealing temperature of 58°C (PSY1, PSY2, CYC-B, and LCY-B), or 60°C (GGDR, VTE-2, VTE-5, and HPPD) for 20 s, and an extension temperature of 72°C for 30 s. Thirty cycles were required for all genes, except for LCY-B, which required 32 cycles. Relative mRNA expression was calculated by the 2 -DDCt method (Villa-Hern andez et al, 2013). A value of 1 was arbitrarily assigned to every replicate of the mRNA expression of the R N genotype, and the relative expression of the other genotypes was calculated using this genotype as reference.…”

Section: Expression Of the Genes Encoding Vitamin E And Carotenoid Bimentioning

confidence: 99%

Antioxidant Balance and Regulation in Tomato Genotypes of Different Color

Vela-Hinojosa¹,

Escalona‐Buendía²,

Mendoza‐Espinoza³

et al. 2019

J. Amer. Soc. Hort. Sci.

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ADDITIONAL INDEX WORDS. anthocyanins, polyphenols, flavonoids, PSY ABSTRACT. Antioxidants, antioxidant capacity, and the expression of isoprenoid metabolism-related genes and two pigmentation-related transcription factors were studied in four native and four hybrid tomato (Solanum lycopersicum) genotypes with different-colored fruit. Red fruit genotypes were associated with greater lycopene, b-carotene, lipophilic antioxidant capacity, and greater chromoplast-specific lycopene b-cyclase (CYC-B) transcript levels. Orange fruit genotypes had greater concentrations of tocopherols and greater transcript levels of homogentisate phytyl transferase (VTE-2), 1-deoxy-D-xylulose phosphate synthase (DXS), and 4-hydroxyphenylpyruvate dioxygenase (HPPD). The yellow fruit genotype was greater in total polyphenol and hydrophilic antioxidant capacity with greater expression of geranylgeranyl reductase (GGDR), phytol kinase (VTE-5), phytoene synthase (PSY) 2, lycopene b-cyclase (LCY-B), SlNAC1, and SINAC4. Greater levels of individual antioxidants were associated with specific coloration of tomato fruit. Moreover, the negative correlations between the expression of PSY1 and VTE-5, and between lycopene and chlorophyll, suggest a balance between carotenoids, tocopherols, and chlorophylls. The results of this study support either the direct commercialization of tomatoes with different color fruit or use of their genotypes in breeding programs to increase antioxidant levels among existing cultivars.

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Section: Expression Of the Genes Encoding Vitamin E And Carotenoid Bimentioning

confidence: 99%

Antioxidant Balance and Regulation in Tomato Genotypes of Different Color

Vela-Hinojosa¹,

Escalona‐Buendía²,

Mendoza‐Espinoza³

et al. 2019

J. Amer. Soc. Hort. Sci.

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“…In plants, auxin is the major phytohormone and growth factor controlling the cell cycle, division, elongation, differentiation, growth, and development (Teale et al, 2006 ). Auxin and growth factors can activate the TOR signaling pathway to modulate specific mRNA transcription, translation, translation re-initiation, and selective protein synthesis by phosphorylating key downstream regulators such as S6 ribosomal protein kinase (S6K), S6 ribosomal protein, and eukaryotic initiation factor 4E (eIF4E; Dinkova et al, 2000 ; Beltrán-Peña et al, 2002 ; Bögre et al, 2013 ; Schepetilnikov et al, 2013 ; Villa-Hernández et al, 2013 ). Our recent observations indicate that many auxin signaling associated genes are differentially expressed in Arabidopsis seedlings treated with AZD8055 (one of asTORis; Dong et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Target of Rapamycin Is a Key Player for Auxin Signaling Transduction in Arabidopsis

Deng

Zheng

et al. 2016

Front. Plant Sci.

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Target of rapamycin (TOR), a master sensor for growth factors and nutrition availability in eukaryotic species, is a specific target protein of rapamycin. Rapamycin inhibits TOR kinase activity viaFK506 binding protein 12 kDa (FKBP12) in all examined heterotrophic eukaryotic organisms. In Arabidopsis, several independent studies have shown that AtFKBP12 is non-functional under aerobic condition, but one study suggests that AtFKBP12 is functional during anaerobic growth. However, the functions of AtFKBP12 have never been examined in parallel under aerobic and anaerobic growth conditions so far. To this end, we cloned the FKBP12 gene of humans, yeast, and Arabidopsis, respectively. Transgenic plants were generated, and pharmacological examinations were performed in parallel with Arabidopsis under aerobic and anaerobic conditions. ScFKBP12 conferred plants with the strongest sensitivity to rapamycin, followed by HsFKBP12, whereas AtFKBP12 failed to generate rapamycin sensitivity under aerobic condition. Upon submergence, yeast and human FKBP12 can significantly block cotyledon greening while Arabidopsis FKBP12 only retards plant growth in the presence of rapamycin, suggesting that hypoxia stress could partially restore the functions of AtFKBP12 to bridge the interaction between rapamycin and TOR. To further determine if communication between TOR and auxin signaling exists in plants, yeast FKBP12 was introduced into DR5::GUS homozygous plants. The transgenic plants DR5/BP12 were then treated with rapamycin or KU63794 (a new inhibitor of TOR). GUS staining showed that the auxin content of root tips decreased compared to the control. DR5/BP12 plants lost sensitivity to auxin after treatment with rapamycin. Auxin-defective phenotypes, including short primary roots, fewer lateral roots, and loss of gravitropism, occurred in DR5/BP12 plants when seedlings were treated with rapamycin+KU63794. This indicated that the combination of rapamycin and KU63794 can significantly inhibit TOR and auxin signaling in DR5/BP12 plants. These studies demonstrate that TOR is essential for auxin signaling transduction in Arabidopsis.

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“…Adoption of the hexagonal II phase of membrane (Ishibashi, Koda, Zheng, Yuasa & Iwaya-Inoue, 2013, Verkleij, De Maagd, Leunissen-Bijvelt & De Kruijff, 1982) contributes to substantial cytoplasmic leakage upon hydration (Bramlage et al , 1978, Lyons, 1973). Membranes must quickly reorganize from a hexagonal II phase to a lamellar phase in order to restore normal function and to terminate leakage of cellular components(Villa-Hernandez, Dinkova, Aguilar-Caballero, Rivera-Cabrera, Sanchez de Jimenez & Perez-Flores, 2013). Against this background, the widely accepted model for imbibitional chilling injury is that a low temperature disrupts membrane reorganization.…”

Section: Introductionmentioning

confidence: 99%

How membranes organize during seed germination: three patterns of dynamic lipid remodelling define chilling resistance and affect plastid biogenesis

2015

Plant Cell & Environment

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Imbibitional chilling injury during germination causes agricultural losses but this can be overcome by osmopriming. It remains unknown how membranes reorganize during germination. Herein, we comparatively profiled changes of membrane lipids during imbibition under normal and chilling temperatures in chilling-tolerant and -sensitive soybean seeds. We found three patterns of dynamic lipid remodelling during the three phases of germination. Pattern 1 involved a gradual increase in plastidic lipids during phases I and II, with an abrupt increase during phase III. This abrupt increase was associated with initiation of photosynthesis. Pattern 3 involved phosphatidic acid (PA) first decreasing, then increasing, and finally decreasing to a low level. Pattern 1 and 3 were interrupted in chilling-sensitive seeds under low temperature, which lead a block in plastid biogenesis and accumulation of harmful PA respectively. However, they were rescued and returned to their status under a normal temperature after polyethylene glycol (PEG) osmopriming. We specifically inhibited phospholipase D (PLD)-mediated PA formation in chilling-sensitive seeds of soybean, cucumber, and pea and found their germination under low temperature was significantly improved. These results indicate that membranes undergo specific and functional reorganization of lipid composition during germination and demonstrate that PLD-mediated PA causes imibibitional chilling injury.

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Regulation of ribosome biogenesis in maize embryonic axes during germination

Cited by 12 publications

References 60 publications

Antioxidant Balance and Regulation in Tomato Genotypes of Different Color

Antioxidant Balance and Regulation in Tomato Genotypes of Different Color

Target of Rapamycin Is a Key Player for Auxin Signaling Transduction in Arabidopsis

How membranes organize during seed germination: three patterns of dynamic lipid remodelling define chilling resistance and affect plastid biogenesis

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