Regulation of Ribosomal RNA Gene Transcription during Retinoic Acid-Induced Differentiation of Mouse Teratocarcinoma Cells

Datta, Prasun K.; Budhiraja, Shalini; Reichel, Ronald; Jacob, Samson T.

doi:10.1006/excr.1996.3446

Cited by 19 publications

(9 citation statements)

References 64 publications

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“…7 These studies strongly suggest that the loss of UBF is causative in rDNA inactivation and demonstrate that pseudo-silencing occurs under biologically relevant conditions. As UBF expression decreases substantially during terminal differentiation in a wide range of systems, 61,[65][66][67][68][69] we propose that modulation of UBF is a general mechanism for rDNA silencing during development. It is worthy to note however that others have correlated changes in CpG methylation with certain models of differentiation 61 suggesting that both methylation dependent and independent mechanisms exist for rDNA inactivation during development.…”

Section: The Role Of Ubf In Regulation Of Active Gene Number During Dmentioning

confidence: 86%

The role of UBF in regulating the structure and dynamics of transcriptionally active rDNA chromatin

Sanij¹,

Hannan²

2009

Epigenetics

104

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Somatic cells have approximately 200 hundred copies of ribosomal RNA (rRNA) genes (rDNA) per haploid genome of which only a fraction are transcribed by RNA polymerase I (Pol I) at any given time. Thus regulation of rDNA transcription involves controlling both the number of active genes as well as the rate of transcription per gene. How and why a majority of rDNA are silenced is unclear but recent studies indicate that in addition to controlling rRNA synthesis, rDNA silencing plays an essential role in maintaining the genetic stability of rDNA repeats and influences cellular events such as aging and senescence. In this review we discuss the role of the cytoarchitectural transcription factor UBF in determining and maintaining the active (euchromatic) state of rDNA in mammals. In particular we discuss evidence to suggest that UBF dynamically regulates the active rRNA gene pool during differentiation and malignancy.

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Section: The Role Of Ubf In Regulation Of Active Gene Number During Dmentioning

confidence: 86%

The role of UBF in regulating the structure and dynamics of transcriptionally active rDNA chromatin

Sanij¹,

Hannan²

2009

Epigenetics

104

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“…During F9 cell differentiation induced by retinoic acid, rapidly growing teratocarcinoma cells are converted into various cell types. These cells display a low rate of proliferation and a downregulation of rDNA expression [26]. Cell growth in non-lymphoid cells stimulates ribosomal gene transcription [27].…”

Section: Discussionmentioning

confidence: 99%

Ku80 is required but not sufficient for Gα13-mediated endodermal differentiation in P19 embryonic carcinoma cells

Kanungo

Wang

Malbon

2004

Biochemical and Biophysical Research Communications

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We have shown that a constitutively active Galpha13 (Galpha13Q226L) induces differentiation in P19 embryonic carcinoma cells to an endodermal phenotype. In this report, we demonstrate that Ku, a heterodimer of p80 (Ku80) and p70 (Ku70), is upregulated in P19 cells overexpressing Galpha13Q226L. Ku is the regulatory subunit of the DNA-dependent protein kinase and is primarily involved in DNA repair and recombination. Ku80 also is a somatostatin receptor. We show that while overexpression of Ku80 drastically reduced P19 cell proliferation, it was not sufficient to induce endodermal differentiation. However, coexpression of Galpha13Q226L and an antisense Ku80 abrogated the retarded growth rate and endodermal differentiation observed in cells expressing only Galpha13Q226L. Overexpression of Galpha13Q226L or Ku80 downregulated RNA polymerase I-mediated transcriptional activity and overexpression of antisense Ku80 restored the activity to control level. These results suggest that Ku80 is required for Galpha13-mediated endodermal differentiation in P19 cells.

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“…The recent reports that IRS-1 can translocate to the nucleus and bind UBF1 (58, 62) provide a molecular explanation for the effect of IRS-1 on cell and body size. During differentiation (which is inhibited by IRS-1), an inhibition of RNA polymerase I activity (10,11,28,61) and a decrease in cell size (63) occur. In terminally differentiated cells, UBF1 is no longer detectable (11,61), and the nucleolus (which is the site of rRNA synthesis) involutes (50).…”

mentioning

confidence: 99%

Role of Pescadillo and Upstream Binding Factor in the Proliferation and Differentiation of Murine Myeloid Cells

Prisco

Maiorana

Guerzoni

et al. 2004

Molecular and Cellular Biology

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Regulation of Ribosomal RNA Gene Transcription during Retinoic Acid-Induced Differentiation of Mouse Teratocarcinoma Cells

Cited by 19 publications

References 64 publications

The role of UBF in regulating the structure and dynamics of transcriptionally active rDNA chromatin

The role of UBF in regulating the structure and dynamics of transcriptionally active rDNA chromatin

Ku80 is required but not sufficient for Gα13-mediated endodermal differentiation in P19 embryonic carcinoma cells

Role of Pescadillo and Upstream Binding Factor in the Proliferation and Differentiation of Murine Myeloid Cells

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