Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIα

Abstract

Background: cAMP-induced phosphorylation of RhoA has been considered to inhibit RhoA signaling, causing cell rounding. Results: Knockdown of RhoGDI␣ blocks cAMP-induced cell rounding, and RhoGDI␣-WT expression but not RhoGDI␣-S174A expression recovers. Conclusion: Phosphorylation of RhoGDI␣ likely inhibits RhoA by stabilizing a active RhoA-RhoGDI␣ complex. Significance: This may underlie G s /cAMP-induced cross-talk with G q /G 13 /RhoA signaling.

Cyclic AMP prevents decrease of phosphorylated ezrin/radixin/moesin and chloride intracellular channel 5 expressions in injured podocytes

“…*p \ 0.05 compared with 0 h or control group, # p \ 0.05 compared with PAN group. 2Me: 2Me-cAMP, pCPT: pCPT-cAMP PKA-dependent or -independent manner to activate RhoA [25]. Our results supported cAMP/PKA signaling activated RhoA.…”

Regulating Rho GTPases and their regulators

“…PKA-mediated phosphorylation on Ser174 of RhoGDI1 stabilizes a RhoA-RhoGDI1 complex, thereby inhibiting RhoA signalling 126 . This mechanism might complement the PKA-mediated phosphoryl ation of RhoA described at the beginning of this Review, which enhances its interaction with RhoGDI.…”

Tunable Visible and Near‐IR Photoactivation of Light‐Responsive Compounds by Using Fluorophores as Light‐Capturing Antennas

“…cAMP-induced activation of PKA is known to promote dramatic morphological changes in cells, including loss of stress fibers and cell-rounding. [13] In the absence of illumination, Cbl-cAMP has no effect on the morphology of REF52 cells. Upon illumination in the presence of Cbl-cAMP , REF52 cells undergo the expected loss of their stress fiber content as well as cell shrinking and rounding (Fig.…”