Regulation of prostacyclin synthase expression and prostacyclin content in the pig endometrium

Morawska, E.; Kaczmarek, Monika M.; Blitek, Agnieszka

doi:10.1016/j.theriogenology.2012.07.028

Cited by 21 publications

(46 citation statements)

References 54 publications

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“…An alternative source of gene regulation in endometrial epithelia requires the down‐regulation of Progesterone receptor by progesterone, which eliminates Progesterone receptor‐dependent inhibition of the expression of progesterone‐regulated genes (Geisert et al, ), such as Uteroferrin (Knight, Bazer, & Wallace, ), Uterine trypsin/plasmin inhibitor protein (Fazleabas et al , ), Retinol‐binding protein (Adams, Bazer, & Roberts, ), Fibroblast growth factor 7 (FGF7) (Ka, Spencer, Johnson, & Bazer, ), VEGF (Welter, Wollenhaupt, Tiemann, & Einspanier, ), swine Leukocyte antigens, and B2‐macroglobulin (Joyce et al, ). Progesterone also increases endometrial expression of Homeobox A10, Prostaglandin endoperoxide synthase 2, and PGI2 synthase (Blitek, Kiewisz, Waclawik, Kaczmarek, & Ziecik, ; Morawska, Kaczmarek, & Blitek, ).…”

Section: The Conceptus‐maternal Interface and The Pro‐inflammatory Enmentioning

confidence: 99%

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Wacławik

Kaczmarek

Blitek

et al. 2017

Molecular Reproduction Devel

102

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Porcine conceptuses secrete pregnancy-recognition signals (estrogens, including estradiol-17β) that inhibit luteolysis, thereby prolonging progesterone production by corpora lutea. The supportive mechanism by which the conceptus also inhibits luteolysis is by shifting endometrial prostaglandin (PG) synthesis to luteoprotective PGE2.Progesterone stimulates endometrial production of factors that are essential for conceptus development. Priming the uterus by progesterone and loss of progesterone receptors from the uterine epithelium by Day 10-12 after estrus are key for achieving endometrial receptivity for implantation. Conceptus implantation involves a series of events, many resembling the inflammatory reaction, that are greatly influenced by cytokines, growth factors, and prostaglandins. We herein present a novel, dual role for PGF2α in corpora lutea that depends on the acquisition of luteolytic sensitivity, based on the knowledge that PGF2α triggers pathways involved in luteolysis during the estrous cycle or/and may have an alternative function in maintaining progesterone synthesis during pregnancy. We also point out a new role for PGF2α that, together with PGE2, can act as embryonic signal mediators. PGF2α, which until recently was considered undesirable for promoting pregnancy, is now known to stimulate conceptus-maternal interactions and angiogenesis in the endometrium. This function is in line with other important prostaglandin functions, such as stimulating adhesion of trophoblasts (PGE2, PGI2) as well as endometrial vascular functions and trophoblast cell proliferation (PGI2).

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Section: The Conceptus‐maternal Interface and The Pro‐inflammatory Enmentioning

confidence: 99%

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Wacławik

Kaczmarek

Blitek

et al. 2017

Molecular Reproduction Devel

102

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“…Plasma and urinary levels of 6-keto-prostaglandin F 1α (6-keto-PGF 1α ), a hydration product of PGI 2 , increase during pregnancy (Majed and Khalil, 2012). Also, PGI 2 synthase mRNA and protein expression and 6-keto-PGF 1α levels are elevated in pig endometrium during normal pregnancy (Morawska et al, 2012). However, vascular function studies showed little contribution of PGI 2 to bradykinin-induced relaxation in small subcutaneous arteries from Norm-Preg women (Luksha et al, 2004).…”

Section: Decreased Endothelium-derived Relaxing Factors In Preeclamentioning

confidence: 99%

Mechanisms of Endothelial Dysfunction in Hypertensive Pregnancy and Preeclampsia

Possomato-Vieira

Khalil

2016

Advances in Pharmacology

184

117

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Preeclampsia is a pregnancy-related disorder characterized by hypertension, and could lead to maternal and fetal morbidity and mortality. Although the causative factors and pathophysiological mechanisms are unclear, endothelial dysfunction is a major hallmark of preeclampsia. Clinical tests and experimental research have suggested that generalized endotheliosis in the systemic, renal, cerebral and hepatic circulation could decrease endothelium-derived vasodilators such as nitric oxide, prostacyclin and hyperpolarization factor and increase vasoconstrictors such as endothelin-1 and thromboxane A2, leading to increased vasoconstriction, hypertension and other manifestation of preeclampsia. In search for the upstream mechanisms that could cause endothelial dysfunction, certain genetic, demographic and environmental risk factors have been suggested to cause abnormal expression of uteroplacental integrins, cytokines and matrix metalloproteinases, leading to decreased maternal tolerance, apoptosis of invasive trophoblast cells, inadequate spiral arteries remodeling, reduced uterine perfusion pressure (RUPP), and placental ischemia/hypoxia. RUPP may cause imbalance between the anti-angiogenic factors soluble fms-like tyrosine kinase-1 and soluble endoglin and the pro-angiogenic factors vascular endothelial growth factor and placental growth factor, or stimulate the release of other circulating bioactive factors such as inflammatory cytokines, hypoxia-inducible factor-1, reactive oxygen species, and angiotensin AT1 receptor agonistic autoantibodies. These circulating factors could then target endothelial cells and cause generalized endothelial dysfunction. Therapeutic options are currently limited, but understanding the factors involved in endothelial dysfunction could help design new approaches for prediction and management of preeclampsia.

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“…Endometrial explants (100–110 mg per vial) were collected and preincubated in 2 ml of Medium 199 supplemented with 5% of charcoal-stripped NCS and antibiotics for 2 h, as described recently [17]. Then, the medium was removed, and endometrial tissue was treated with the control medium (phenol red-free Medium 199 containing 5% of charcoal-stripped NCS and antibiotics) or CEM mixed 3:1 with the control medium for 6 and 24 h. All treatments were performed in duplicate for each of six gilts.…”

Section: Methodsmentioning

confidence: 99%

Effect of Conceptus on Transforming Growth Factor (TGF) β1 mRNA Expression and Protein Concentration in the Porcine Endometrium— <i>In Vivo</i> and <i>In Vitro</i> Studies

Blitek

Morawska-Pucinska

Szymańska

et al. 2013

Journal of Reproduction and Development

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Transforming growth factor (TGF) β and its receptors are expressed at the conceptus-maternal interface during early pregnancy in the pig. The present studies were conducted to examine: (1) the effect of conceptus products on TGFβ1 mRNA expression and protein concentration in the porcine endometrium using in vivo and in vitro models, and (2) the effect of TGFβ1 on proliferation of porcine trophoblast cells in vitro. During in vivo experiments, gilts with one surgically detached uterine horn were slaughtered on days 11 or 14 of the estrous cycle and pregnancy. For in vitro studies, endometrial explants and luminal epithelial (LE) cells co-cultured with stromal (ST) cells were treated with conceptus-exposed medium (CEM). Moreover, porcine trophoblast cells were treated with TGFβ1, and the number of viable cells was measured. On day 11, the presence of conceptuses had no effect on TGFβ1 mRNA expression, but decreased the TGFβ1 protein concentration in the connected uterine horn compared with the detached uterine horn. In contrast to day 11, on day 14 after estrus, TGFβ1 mRNA expression and protein content in the endometrium collected from the gravid uterine horn were greater when compared with the contralateral uterine horn. The treatment of endometrial slices with CEM resulted in greater TGFβ1 mRNA expression and protein secretion. LE cells responded to CEM with an increased TGFβ1 mRNA level. Moreover, TGFβ1 stimulated the proliferation of day 14 trophoblast cells. In summary, porcine conceptuses may regulate TGFβ1 synthesis in the endometrium at the time of implantation. TGFβ1, in turn, may promote conceptus development by increasing the proliferation of trophoblast cells.

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Regulation of prostacyclin synthase expression and prostacyclin content in the pig endometrium

Cited by 21 publications

References 54 publications

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Mechanisms of Endothelial Dysfunction in Hypertensive Pregnancy and Preeclampsia

Effect of Conceptus on Transforming Growth Factor (TGF) β1 mRNA Expression and Protein Concentration in the Porcine Endometrium— <i>In Vivo</i> and <i>In Vitro</i> Studies

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