Regulation of potato tuber sprouting

Sonnewald, Sophia; Sonnewald, Uwe

doi:10.1007/s00425-013-1968-z

Cited by 196 publications

(162 citation statements)

References 98 publications

(152 reference statements)

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“…The control of tuber sprouting is a major target in potato breeding because premature tuber sprouting during their lengthy storage leads to important quality and economic loss [41][42][43]. However, the molecular mechanisms controlling dormancy release and tuber sprouting are not yet sufficiently known [42][43][44].…”

Section: Quantitative Profiling Of Phosphorylated Patatin Isoformsmentioning

confidence: 99%

“…However, the molecular mechanisms controlling dormancy release and tuber sprouting are not yet sufficiently known [42][43][44]. The identification and mapping of phosphorylated isoforms Volume of spots for untreated and dephosphorylated protein samples with HF-P were assessed by PDQuest software.…”

Section: Quantitative Profiling Of Phosphorylated Patatin Isoformsmentioning

confidence: 99%

“…In addition, patatin is a family of immunologically indistinguishable isoforms with similar structural properties and thermal conformational stability [21,22]. Overall, extensive heterogeneity in molecular mass (40)(41)(42)(43)(44)(45) and isoelectric point (4.5-5.2) seems to be the most salient differential molecular features among isoforms [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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Identification and Mapping of Phosphorylated Isoforms of the Major Storage Protein of Potato Based on Two- Dimensional Electrophoresis

Bernal¹,

López‐Pedrouso²,

Daprà³

et al. 2017

Advances in Seed Biology

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Protein phosphorylation plays a key role in the synthesis and degradation of dry seed storage proteins. In contrast, no evidence for phosphorylation has been reported to date in vegetative storage proteins (VSPs). The patatin multigene family encodes the major VSP of the potato, Solanum tuberosum L. This study addresses for the first time the identification and mapping of phosphorylated patatin forms based on high-resolution two-dimensional electrophoresis (2-DE) profiles. Patatin isoforms from mature tubers of cultivar Kennebec were separated by 2-DE and subsequently identified by tandem mass spectrometry. In-gel identification and mapping of phosphorylated isoforms were performed using the multiplex phosphoprotein-specific staining Pro-Q DPS. We found that phosphorylation is a ubiquitous post-translational protein modification associated with isoforms of patatin. In addition, protein dephosphorylation with hydrogen fluoride-pyridine coupled to 2-DE was used for quantitative profiling of phosphorylated patatin. This experimental approach showed that patatin comprises multiple isoforms with very different phosphorylation level. Thus, phosphorylation rates over isoforms ranged from 4.6 to 52.3%. Overall, the identification and mapping of differentially phosphorylated patatin opens up new exploratory ways to unravel the molecular mechanisms underlying its mobilization along the tuber life cycle.

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Section: Quantitative Profiling Of Phosphorylated Patatin Isoformsmentioning

confidence: 99%

Section: Quantitative Profiling Of Phosphorylated Patatin Isoformsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Mapping of Phosphorylated Isoforms of the Major Storage Protein of Potato Based on Two- Dimensional Electrophoresis

Bernal¹,

López‐Pedrouso²,

Daprà³

et al. 2017

Advances in Seed Biology

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“…Suppression of tuber sprouting is of significant benefit to the industry for the long-term storage of tubers. Thus, controlling tuber sprouting is another important objective in potato breeding (Sonnewald and Sonnewald, 2014).…”

mentioning

confidence: 99%

Two Cytochrome P450 Monooxygenases Catalyze Early Hydroxylation Steps in the Potato Steroid Glycoalkaloid Biosynthetic Pathway

et al. 2016

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Solanine and a-chaconine, steroidal glycoalkaloids (SGAs) found in potato (Solanum tuberosum), are among the best-known secondary metabolites in food crops. At low concentrations in potato tubers, SGAs are distasteful; however, at high concentrations, SGAs are harmful to humans and animals. Here, we show that POTATO GLYCOALKALOID BIOSYNTHESIS1 (PGA1) and PGA2, two genes that encode cytochrome P450 monooxygenases (CYP72A208 and CYP72A188), are involved in the SGA biosynthetic pathway, respectively. The knockdown plants of either PGA1 or PGA2 contained very little SGA, yet vegetative growth and tuber production were not affected. Analyzing metabolites that accumulated in the plants and produced by in vitro enzyme assays revealed that PGA1 and PGA2 catalyzed the 26-and 22-hydroxylation steps, respectively, in the SGA biosynthetic pathway. The PGA-knockdown plants had two unique phenotypic characteristics: The plants were sterile and tubers of these knockdown plants did not sprout during storage. Functional analyses of PGA1 and PGA2 have provided clues for controlling both potato glycoalkaloid biosynthesis and tuber sprouting, two traits that can significantly impact potato breeding and the industry.

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“…Besides causing the weight loss, sprouting is also highly detrimental to the nutritional status and quality aspects of potatoes (van Es and Hartmans 1987a;Mani et al 2014). Sprouting leads to higher rate of respiration, remobilization of storage compounds in the potato tubers mainly starch and proteins besides causing shrinkage due to loss of water (Sonnewald and Sonnewald 2014). These changes also cause deterioration in processing quality due to loss in mass, decreased turgor, structural change due to growth of sprout tissue and increase in sugar concentrations due to hydrolysis of starch (Burton et al 1992;Davies 1990;van Es and Hartmans 1987b;Daniels-Lake et al 2005).…”

Section: Adverse Consequences Of Sprouting Of Potatoes During Storagementioning

confidence: 99%

Sprout suppression on potato: need to look beyond CIPC for more effective and safer alternatives

2015

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World over, potatoes are being stored at [8][9][10][11][12]. This is the most common way of long-term (up to 6 to 9 months) storage of potatoes. The benefit of storing the potatoes within the temperature range of 8-12°C is minimum accumulation of sugars in stored potato tubers. In sub-temperate, sub-tropical and tropical countries of the world, short-term (3 to 4 months) storage of potatoes is being done by non-refrigerated traditional/on-farm methods. These short-and long-term storage methods keep the stored potatoes suitable not only for table purpose but also for processing. However, once the natural dormancy period of potato is over, the prevailing temperatures in these storage methods favour sprouting and sprout growth. Therefore, use of some sprout suppressant to check the sprout growth becomes essential under these methods of potato storage. CIPC [Isopropyl N-(3-chlorophenyl) carbamate] is the most wide spread and commonly used sprout suppressant on potatoes. CIPC has been in use for more than 50 years and research carried out over such a long period use of CIPC has not only enhanced our understanding of its properties and chemistry but also about the production and toxicological status of its metabolites/ degradation products. Today, various safety issues and concerns have surfaced primarily due to continuous and longterm use of CIPC. This review presents an appraisal on CIPC and explains the reasons for the long-time dependence on this chemical as a potato sprout suppressant. Issues like maximum residue limit and acceptable daily intake limit are being discussed for CIPC. This article brings an update on practical aspects of potato storage, residue levels of CIPC, efficacy of CIPC as sprout suppressant and health and environmental safety issues linked with CIPC and its metabolites. The aim of this article is to find possible solutions, way outs and future plans that can make the sprout suppression of potatoes safer and more risk free.

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Regulation of potato tuber sprouting

Cited by 196 publications

References 98 publications

Identification and Mapping of Phosphorylated Isoforms of the Major Storage Protein of Potato Based on Two- Dimensional Electrophoresis

Identification and Mapping of Phosphorylated Isoforms of the Major Storage Protein of Potato Based on Two- Dimensional Electrophoresis

Two Cytochrome P450 Monooxygenases Catalyze Early Hydroxylation Steps in the Potato Steroid Glycoalkaloid Biosynthetic Pathway

Sprout suppression on potato: need to look beyond CIPC for more effective and safer alternatives

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