Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252

Gavigan, Julie-Ann; Marshall, Leonard M.; Dobson, Alan D. W.

doi:10.1099/00221287-145-10-2931

Cited by 17 publications

(14 citation statements)

References 26 publications

(13 reference statements)

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“…In contrast, neither carbon nor nitrogen limitation led to the triggering of ppk expression (data not shown). These results indicate that the expression of ppk in S. lividans TK24 is triggered when P i becomes limiting in the growth medium, as previously demonstrated in other bacteria (8,9).…”

Section: Resultssupporting

confidence: 70%

Regulation of ppk Expression and In Vivo Function of Ppk in Streptomyces lividans TK24

et al. 2006

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The ppk gene of Streptomyces lividans encodes an enzyme catalyzing, in vitro, the reversible polymerization of the ␥ phosphate of ATP into polyphosphate and was previously shown to play a negative role in the control of antibiotic biosynthesis (H. Chouayekh and M. J. Virolle, Mol. Microbiol. 43:919-930, 2002). In the present work, some regulatory features of the expression of ppk were established and the polyphosphate content of S. lividans TK24 and the ppk mutant was determined. In P i sufficiency, the expression of ppk was shown to be low but detectable. DNA gel shift experiments suggested that ppk expression might be controlled by a repressor using ATP as a corepressor. Under these conditions, short acid-soluble polyphosphates accumulated upon entry into the stationary phase in the wild-type strain but not in the ppk mutant strain. The expression of ppk under P i -limiting conditions was shown to be much higher than that under P i -sufficient conditions and was under positive control of the two-component system PhoR/PhoP. Under these conditions, the polyphosphate content of the cell was low and polyphosphates were reproducibly found to be longer and more abundant in the ppk mutant strain than in the wild-type strain, suggesting that Ppk might act as a nucleoside diphosphate kinase. In light of our results, a novel view of the role of this enzyme in the regulation of antibiotic biosynthesis in S. lividans TK24 is proposed.

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Section: Resultssupporting

confidence: 70%

Regulation of ppk Expression and In Vivo Function of Ppk in Streptomyces lividans TK24

et al. 2006

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“…When compared to the control cells at the end of the starvation phase (T4) the expression of ppk1 was 4.2-fold lower in the treated cells, rising to 3.7-fold higher at after overplus (T6). Upregulation of ppk1 expression would therefore appear to begin after the addition of Pi to Pi-starved M. mazei cells and thus differ from the majority of previous bacterial overplus studies where ppk (and ppx) expression has been shown to begin during the Pi starvation phase, under the control of the PHO regulon [57][58][59][60][61][62][63] .…”

Section: Discussioncontrasting

confidence: 76%

The potential for polyphosphate metabolism in Archaea and anaerobic polyphosphate formation inMethanosarcina mazei

Paula

Chin

Schnürer

et al. 2019

Preprint

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2Inorganic polyphosphate (polyP) is ubiquitous across all forms of life, but the study of its metabolism has been mainly confined to bacteria and yeasts. Few reports detail the presence and accumulation of polyP in Archaea, and little information is available on its functions and regulation. Here, we report that homologs of bacterial polyP metabolism proteins are present across the major taxa in the Archaea, suggesting that archaeal populations may have a greater contribution to global phosphorus cycling than has previously been recognised. We also demonstrate that polyP accumulation can be induced under strictly anaerobic conditions, in response to changes in phosphate (Pi) availability, i.e. Pi starvation, followed by incubation in Pi replete media (overplus), in cells of the methanogenic archaeon Methanosarcina mazei.Pi-starved M. mazei cells increased transcript abundance of the PHO-regulated alkaline phosphatase (phoA) gene and of the high-affinity phosphate transport (pstSCAB-phoU) operon: no increase in polyphosphate kinase 1 (ppk1) transcript abundance was observed.Subsequent incubation of Pi-starved M. mazei cells under Pi replete conditions, led to a 237% increase in intracellular polyphosphate content and a >5.7-fold increase in ppk1 gene transcripts. Ppk1 expression in M. mazei thus appears not to be under classical PHO regulon control.

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“…Their expression patterns suggest they are involved in both the formation and degradation processes in Accumulibacter, although the exact factor(s) triggering their induction in the two phases are not clear. It was suggested that ppk1 was under Pho regulon control in Klebsiella aerogenes (Kato et al, 1993) and Acinetobacter baumannii (Gavigan et al, 1999), with transcription being induced by P i limitation. If control mechanisms were similar in Accumulibacter, ppk1 transcript abundance would be expected to increase in the P i -depleted aerobic famine phase.…”

Section: Discussionmentioning

confidence: 99%

‘Candidatus Accumulibacter’ gene expression in response to dynamic EBPR conditions

McMahon

2010

The ISME Journal

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Enhanced biological phosphorus removal (EBPR) activated sludge communities enriched in 'Candidatus Accumulibacter' relatives are widely used in wastewater treatment, but much remains to be learned about molecular-level controls on the EBPR process. The expression of genes found in the carbon and polyphosphate metabolic pathways in Accumulibacter was investigated using reverse transcription quantitative PCR. During a normal anaerobic/aerobic EBPR cycle, gene expression exhibited a dynamic change in response to external acetate, oxygen, phosphate concentrations and probably internal chemical pools. Anaerobic acetate addition induced expression of genes associated with the methylmalonyl-CoA pathway enabling the split mode of the tricarboxylic acid (TCA) cycle. Components of the full TCA cycle were induced after the switch to aerobic conditions. The induction of a key gene in the glyoxylate shunt pathway was observed under both anaerobic and aerobic conditions, with a higher induction by aeration. Polyphosphate kinase 1 from Accumulibacter was expressed, but did not appear to be regulated by phosphate limitation. To understand how Accumulibacter responds to disturbed electron donor and acceptor conditions, we perturbed the process by adding acetate aerobically. When high concentrations of oxygen were present simultaneously with acetate, phosphate-release was almost completely inhibited, and polyphosphate kinase 1 transcript abundance decreased. Genes associated with the methylmalonylCoA pathway were repressed and genes associated with the aerobic TCA cycle exhibited higher expression under this perturbation, suggesting that more acetyl-CoA was metabolized through the TCA cycle. These findings suggest that several genes involved in EBPR are tightly regulated at the transcriptional level.

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Regulation of polyphosphate kinase gene expression in Acinetobacter baumannii 252

Cited by 17 publications

References 26 publications

Regulation of ppk Expression and In Vivo Function of Ppk in Streptomyces lividans TK24

Regulation of ppk Expression and In Vivo Function of Ppk in Streptomyces lividans TK24

The potential for polyphosphate metabolism in Archaea and anaerobic polyphosphate formation inMethanosarcina mazei

‘Candidatus Accumulibacter’ gene expression in response to dynamic EBPR conditions

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