Regulation of PMP22 mRNA by G3BP1 affects cell proliferation in breast cancer cells

Winslow, Sofia; Leandersson, Karin; Larsson, Christer

doi:10.1186/1476-4598-12-156

Cited by 57 publications

(67 citation statements)

References 36 publications

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“…Winslow et al demonstrated that PMP22 protein could regulate cell spreading and affect cell proliferation in breast cancer cells [16]. Furthermore, it has been demonstrated previously that PMP22 can interact with members of the transforming growth factor-beta (TGF-β) family [17].…”

Section: Discussionmentioning

confidence: 99%

Suppression of peripheral myelin protein 22 (PMP22) expression by miR29 inhibits the progression of lung cancer

Qu¹,

Zhu²,

Tao³

et al. 2015

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PMP22 is recently recognized as a key player in a variety of prevalent cancers. In this study, we sought to explore the correlation of peripheral myelin protein 22 (PMP22) expression with cell proliferation, invasion and apoptosis in lung cancer cell line. miR29 was transfected into Anip973 lung adenocarcinoma cell line to interfere the expression of PMP22 using Lipofectamine ® 2000 reagent. Real-time polymerase chain reaction (RT-PCR) and Western blot were performed to determine the expression level of PMP22 at mRNA and protein level. Then MTT, Matrigel transwell assay and flow cytometry were respectively used to explore the proliferation, invasion and apoptosis in Anip973 lung adenocarcinoma cell line in vitro. miR29 could significantly down-regulate the expression level of PMP22 in Anip973 cells not only at mRNA level but also at protein level. Moreover, the proliferation rate, invasive cell number and apoptosis rate of Anip973 cells in miR29 transfected group significantly decreased compared with blank group, while no significant difference existed between control group and blank group. Our study found that suppression of PMP22 expression could inhibit cell proliferation, invasion and apoptosis in lung cancer cells. All these findings suggest that PMP22 may be involved in progression of lung cancer and could be a new therapeutic target for this disease.

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Section: Discussionmentioning

confidence: 99%

Suppression of peripheral myelin protein 22 (PMP22) expression by miR29 inhibits the progression of lung cancer

Qu¹,

Zhu²,

Tao³

et al. 2015

neo

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“…Furthermore, the generation of G3BP1 knockout (KO) mice supported the role of G3BP1 in cell survival and proliferation [32]. Moreover, G3BP1 (but not G3BP2) is involved in the cellular proliferation of various breast cancer cell lines through a regulatory effect on peripheral myelin protein 22 (PMP22) [33].…”

Section: Expression Of G3bps and Their Activitymentioning

confidence: 97%

Rasputin a decade on and more promiscuous than ever? A review of G3BPs

Alam

Kennedy

2019

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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“…PMP22 is expressed primarily in neural crest derived cell lineages (including melanocytes), however expression of PMP22 has also been associated with cell proliferation and survival in breast cancer [3–5]. To date no target genes of miR-4731 have been functionally validated.…”

Section: Introductionmentioning

confidence: 99%

The ‘melanoma-enriched’ microRNA miR-4731-5p acts as a tumour suppressor

et al. 2016

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We previously identified miR-4731-5p (miR-4731) as a melanoma-enriched microRNA following comparison of melanoma with other cell lines from solid malignancies. Additionally, miR-4731 has been found in serum from melanoma patients and expressed less abundantly in metastatic melanoma tissues from stage IV patients relative to stage III patients. As miR-4731 has no known function, we used biotin-labelled miRNA duplex pull-down to identify binding targets of miR-4731 in three melanoma cell lines (HT144, MM96L and MM253). Using the miRanda miRNA binding algorithm, all pulled-down transcripts common to the three cell lines (n=1092) had potential to be targets of miR-4731 and gene-set enrichment analysis of these (via STRING v9.1) highlighted significantly associated genes related to the ‘cell cycle’ pathway and the ‘melanosome’. Following miR-4731 overexpression, a selection (n=81) of pull-down transcripts underwent validation using a custom qRT-PCR array. These data revealed that miR-4731 regulates multiple genes associated with the cell cycle (e.g. CCNA2, ORC5L, and PCNA) and the melanosome (e.g. RAB7A, CTSD, and GNA13). Furthermore, members of the synovial sarcoma X breakpoint family (SSX) (melanoma growth promoters) were also down-regulated (e.g. SSX2, SSX4, and SSX4B) as a result of miR-4731 overexpression. Moreover, this down-regulation of mRNA expression resulted in ablation or reduction of SSX4 protein, which, in keeping with previous studies, resulted in loss of 2D colony formation. We therefore speculate that loss of miR-4731 expression in stage IV patient tumours supports melanoma growth by, in part; reducing its regulatory control of SSX expression levels.

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Regulation of PMP22 mRNA by G3BP1 affects cell proliferation in breast cancer cells

Cited by 57 publications

References 36 publications

Suppression of peripheral myelin protein 22 (PMP22) expression by miR29 inhibits the progression of lung cancer

Suppression of peripheral myelin protein 22 (PMP22) expression by miR29 inhibits the progression of lung cancer

Rasputin a decade on and more promiscuous than ever? A review of G3BPs

The ‘melanoma-enriched’ microRNA miR-4731-5p acts as a tumour suppressor

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