Regulation of plasmid replication.

Scott, June R.

doi:10.1128/mmbr.48.1.1-23.1984

Cited by 154 publications

(42 citation statements)

References 153 publications

(237 reference statements)

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“…The low copy-number plasmids Pi and F' have been estimated fo be present in about one copy per bacferial chromosome (Scott, 1984), and thus the fluorescence in HP Fig. 3.…”

Section: Discussionmentioning

confidence: 99%

Direct visualization of plasmid DNA in bacterial cells

Eliasson

Bernander

Dasgupta

et al. 1992

Molecular Microbiology

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The direct visualization of plasmid DNA inside Escherichia coli cells is demonstrated using phase-fluorescence microscopy of DAPI (4',6-diamidino-2-phenylindole)-stained bacteria. Small as well as large plasmids could be detected, both in minicells and in cells of larger size. For large plasmids, even single molecules appeared to be within the detection limit. The fluorescence generated from monomers of small plasmids was probably below this limit, and for these plasmids the observed signals may represent aggregates. The distribution of the fluorescence foci might reflect specific plasmid positioning during partition and/or replication.

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“…The low copy-number plasmids Pi and F' have been estimated fo be present in about one copy per bacferial chromosome (Scott, 1984), and thus the fluorescence in HP Fig. 3.…”

Section: Discussionmentioning

confidence: 99%

Direct visualization of plasmid DNA in bacterial cells

Eliasson

Bernander

Dasgupta

et al. 1992

Molecular Microbiology

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“…E. L. Larsen. unpublished data) is an Rl-derived vector with a copy number ot one to two per host chromosome (Scott, 1984). It contains the Rl origin of replication, the bta gene of Tn3, and unique cloning sites for the restriction enzymes SamHl, Cla\ and H/Vidlll.…”

Section: Plasmid Pjel 109mentioning

confidence: 99%

Expression of the Escherichia coli dam gene

Løbner‐Olesen

Boye

Marinus

1992

Molecular Microbiology

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The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, and P5 was in the urf74.3-dam intergenic region. The nucleotide sequence of 2280 bp of DNA containing P1 and P2 was determined and shown to have the potential to encode a protein of approximately 16 kDa between P1, P2 and the aroB gene. This 16 kDa open reading frame has been identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam. The transcriptional start points of the promoters were determined. A comparison of their nucleotide sequences suggested that P1-P4 were all recognized by the sigma 70 subunit of the RNA polymerase.

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“…The copy number of bacterial plasmids is strictly controlled in host cells through a variety of negatively acting regulatory mechanisms (for review, see Scott, 1984;Novick, 1987). In plasmids R6K, RK2 and P1, interactions in trans between short repetitive DNA sequences, designated iterons, control their copy number (McEachern et al, 1989;Abeles and Austin, 1991;Kittell and Helinski, 1991; for review, see Nordström, 1990).…”

Section: Introductionmentioning

confidence: 99%

Copy number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA binding at a specific RNA site

Asano

Mizobuchi

1998

The EMBO Journal

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Replication of a low-copy-number IncIα plasmidColIb-P9 depends on expression of the repZ gene encoding the replication initiator protein. repZ expression is negatively controlled by the small antisense Inc RNA, and requires formation of a pseudoknot in the RepZ mRNA consisting of stem-loop I, the Inc RNA target, and a downstream sequence complementary to the loop I. The loop I sequence comprises 5Ј-rUUGGCG-3Ј, conserved in many prokaryotic antisense systems, and was proposed to be the important site of copy number control. Here we show that the level of repZ expression is rate-limiting for replication and thus copy number, by comparing the levels of repZ expression and copy number from different mutant ColIb-P9 derivatives defective in Inc RNA and pseudoknot formation. Kinetic analyses using in vitro transcribed RNAs indicate that Inc RNA binding and the pseudoknot formation are competitive at the level of initial base paring to loop I. This initial interaction is stimulated by the presence of the loop U residue in the 5Ј-rUUGGCG-3Ј motif. These results indicate that the competition between the two RNA-RNA interactions at the specific site is a novel regulatory mechanism for establishing the constant level of repZ expression and thus copy number.

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Regulation of plasmid replication.

Cited by 154 publications

References 153 publications

Direct visualization of plasmid DNA in bacterial cells

Direct visualization of plasmid DNA in bacterial cells

Expression of the Escherichia coli dam gene

Copy number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA binding at a specific RNA site

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