Regulation of Plasma Calcium in Man: The Influence of Parathyroid Hormone and Calcitonin

Gudmundsson, T.V.; Woodhouse, Nicholas

doi:10.1159/000178222

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…Nuclear labeling with [''Hlthymidine at 1 h after injection was invariably confined to cells similarly located (Figs. 6,[8][9][10][11]. With [''Hlthymidine, the only cells labeled 1 h after injection in bone populations were classically described as osteoprogenitor cells (17,19).…”

Section: Suspected Targets In Calcifiedmentioning

confidence: 99%

“…bv, Blood vessel; ob, osteoblast . DISCUSSION Two major hormones, parathyroid hormone and calcitonin, are known to have antagonistic effects on the level of serum calcium (9,16) and consequently on the regulation of bone homeostasis. In this study we have prepared `25 I-labeled salmon calcitonin by a method known to preserve its biological activity (8,14,15).…”

Section: Warshawsky Et Almentioning

confidence: 99%

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Direct in vivo demonstration by radioautography of specific binding sites for calcitonin in skeletal and renal tissues of the rat.

Warshawsky¹,

Goltzman²,

Rouleau³

et al. 1980

The Journal of Cell Biology

202

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An in vivo binding assay using radioautography was employed to visualize calcitonin receptors in rat tissues . At 2 min after intravenous injection of biologically active '25 I-salmon calcitonin, free hormone was separated from bound hormone by intracardiac perfusion with lactated Ringer's followed by fixation with 2 .5% glutaraldehyde . Various tissues were removed and processed for light and electron microscope radioautography . These were compared to tissues removed from animals that received identical amounts of labeled hormone with a large excess of unlabeled calcitonin. Among the tissues investigated, kidney and bone demonstrated labeling . In kidney, most silver grains were located over vesicles below the brush border of cells of the proximal convoluted tubules . These grains were still present after simultaneous injection of excess unlabeled hormone and most likely represented binding to sites involved with ingestion and degradation of hormone from the urinary filtrate . In contrast, grains localized to the basal surfaces of distal convoluted tubule cells were significantly reduced in number in control animals and represented sites of saturable, specific hormone binding . In bone, specific binding sites were found only at the periphery of osteoclasts. These labeled cells were located at resorption sites examined in tibia, humerus, and alveolar bone . This demonstration of the localization of ' 25 I-calcitonin in situ provides a new approach for studying the interaction of calciumregulating hormones with their target cells .

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Section: Suspected Targets In Calcifiedmentioning

confidence: 99%

Section: Warshawsky Et Almentioning

confidence: 99%

Direct in vivo demonstration by radioautography of specific binding sites for calcitonin in skeletal and renal tissues of the rat.

Warshawsky¹,

Goltzman²,

Rouleau³

et al. 1980

The Journal of Cell Biology

202

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“…Since the first parathyroidectomy (PTX) for hyperparathyroidism (HPT) by Felix Mandl in 1925 and subsequent reoperation for recurrent disease [1], the full spectrum of primary HPT has tilted. The widespread use of multichannel analyzers and the breakthrough of the parathormone (PTH) radioimmunoassay [2] followed by the more accurate chemoluminescent assay [3] led to early recognition of more and more cases at an earlier stage of the disease, all treated by bilateral neck exploration, with identification of four or more parathyroid glands; there was a success rate of more than 95.0% [4][5][6] (97.6% in our experience) [7]. Successful PTX requires an experienced surgeon to find, recognize, and excise the abnormal parathyroid glands.…”

mentioning

confidence: 99%

Unilateral Surgery for Hyperparathyroidism: Indications, Limits, and Late Results—New Philosophy or Expensive Selection without Improvement of Surgical Results?

et al. 2004

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We assessed the "late" results after unilateral parathyroidectomy (PTX) performed for selected indications. From October 1998 throughout March 2001 we operated on 454 patients for hyperparathyroidism (HPT). A positive unifocal (99m)tc-MIBI scan was required for the unilateral approach to be used. Intact parathormone (PTH) measurements were done intraoperatively. Postoperative calcium and PTH serum levels of unilaterally operated patients were checked. Follow-up has been 16.2 months (range 6-40 months). Of the 454 patients, 336 (74.0%) were not eligible for the unilateral approach; and 125 (27.5%) of the 454 patients had renal HPT. Among the 329 patients with primary HPT, 125 (38.0%) were excluded for well established reasons, and in 77 other cases (23.5%) preoperative imaging results did not allow the unilateral approach. Altogether, 126 patients (38.3%) with primary HPT were selected for the unilateral approach. Of the 126 unilateral operations, 8 (6.3%) had to be converted to a bilateral procedure. Among the 118 patients with a unilateral approach, 3 patients have been reoperated for overlooked contralateral disease, and 13 dropped out of the study. A total of 102 postoperative calcium and PTH serum late levels are known: 90 (88.2%) patients had normal levels; 10 (9.8%) had a high PTH level, and 2 (1.9%) had high ionized calcium levels. The failure rate in selected cases was 4.2% (5/118) (three were reoperated, and two had a supranormal postoperative ionized Ca level). Even with stringent indications, the late results of unilateral surgery (95.8% cure rate) barely matched those of conventional bilateral surgery (97.6% cure rate). The economic impact of such a surgical strategy should be clarified.

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Parathyroid Hormone, Calcitonin, and Vitamin D

MacIntyre

1974

Current Topics in Experimental Endocrinology

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Regulation of Plasma Calcium in Man: The Influence of Parathyroid Hormone and Calcitonin

Cited by 5 publications

References 39 publications

Direct in vivo demonstration by radioautography of specific binding sites for calcitonin in skeletal and renal tissues of the rat.

Direct in vivo demonstration by radioautography of specific binding sites for calcitonin in skeletal and renal tissues of the rat.

Unilateral Surgery for Hyperparathyroidism: Indications, Limits, and Late Results—New Philosophy or Expensive Selection without Improvement of Surgical Results?

Parathyroid Hormone, Calcitonin, and Vitamin D

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