Regulation of plants' phosphate uptake in common mycorrhizal networks: Role of intraradical fungal phosphate transporters

Walder, Florian; Böller, Thomas; Wiemken, Andres; Courty, Pierre‐Emmanuel

doi:10.1080/15592324.2015.1131372

Cited by 44 publications

(35 citation statements)

References 19 publications

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“…Additional direct evidence comes from an early study on the expression of P‐transporter genes in three Solanaceous species which showed an induction of AMF‐inducible PT4 in leaves and roots of strongly P‐starved eggplants, but not in pepper and N. tabacum (Chen et al ), and a very weak expression of PT5 was also observed in P‐deprived tomato leaves (Chen et al ), and of the AMF‐inducible PT3 in maize leaves (Nagy et al ). Expression of genes known to be AMF‐induced in other tissues were also reported for the AMF inducible Pht1 gene SbPT11 from sorghum, in reproductive tissue (stamen and pistil), and for SbPT11 , SbPT9, LuPT5 and LuPT8 in leaves of sorghum and flax (Walder et al ) all consistent with our findings that PT5 is not only a mycorrhizal marker in roots, but also an indicator of P‐starvation in leaves in N. attenuata . Furthermore, the AMF‐inducible PTs LjPT4 ( L. japonicus ) and MtPT4 ( M. truncatula ) were found to be expressed in non‐inoculated roots (Volpe et al ).…”

Section: Discussionsupporting

confidence: 91%

“…In this context it is important to note that though the overall pattern was similar between the two inocula, the absolute levels as well as the strength of induction differed, which is in agreement with previous studies indicating that different inoculum types can lead to different plant responses (Lopez-Raez et al 2010;Rivero et al 2015;Walder et al 2015;Zubek et al 2015;Walder et al 2016).…”

Section: Discussionsupporting

confidence: 90%

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Nicotiana attenuata's capacity to interact with arbuscular mycorrhiza alters its competitive ability and elicits major changes in the leaf transcriptome

Wang

Wilde

Baldwin

et al. 2018

JIPB

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To study the local and systemic effects of arbuscular mycorrhizal fungal (AMF) colonization, Nicotiana attenuata plants impaired in their interactions with AMF due to silencing of a calcium- and calmodulin dependent protein kinase (inverted repreat (ir)CCaMK) were grown competitively in pairs with empty vector (EV) plants, with and without two different types of inoculum. When inoculated, EV plants strongly outperformed irCCaMK plants. Foliar transcript profiling revealed that AMF colonization significantly changed gene expression of P-starvation and -transporter genes in irCCaMK plants. The Pht1 family phosphate transporter NaPT5 was not only specifically induced in roots after AMF colonization, but also in leaves of AMF-colonized irCCaMK plants, and in plants grown under low Pi conditions in the absence of AMF. The P-starvation signature of inoculated irCCaMK plants corresponded with increases in selected amino acids and phenolic compounds in leaves. We also found a strong AMF-induced increase in amino acids and phenolic metabolites in roots. Plants impaired in their interactions with AMF clearly have a fitness disadvantage when competing for limited soil nutrients with a fully functional isogenic line. The additional role of the AMF-induced Pht1 family transporter NaPT5 in leaves under P-starvation conditions will require further experiments to fully resolve.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Nicotiana attenuata's capacity to interact with arbuscular mycorrhiza alters its competitive ability and elicits major changes in the leaf transcriptome

Wang

Wilde

Baldwin

et al. 2018

JIPB

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“…In R. irregulare , the Pht1 family was divided into four different clusters named after the S. cerevisiae sequences therein (Supplementary Figure S4). ScPHO84 -like cluster groups had putative high affinity P transporters as ScPHO84 and contained four putative R. irregulare sequences previously described ( RiPT1, RiPT2, RiPT3 , and RiPT4 ; Fiorilli et al, 2013; Walder et al, 2016). We found complete and functional sequences only for RiPT1 and RiPT3 .…”

Section: Resultsmentioning

confidence: 94%

“…Fiorilli et al (2013) showed a slight down-regulation of RiPT1 when exposed to higher P concentrations, and Walder et al (2016) reported a positive correlation of RiPT5 transcript levels and Pi acquisition in Sorghum. In our study, only RiPT5 was slightly regulated by P levels, with up-regulation in concentrations higher than 50 ppm in non-treated conditions.…”

Section: Discussionmentioning

confidence: 94%

“…Fungal Pht1 genes are known to be expressed in the intraradical phase (Harrison and van Buuren, 1995; Benedetto et al, 2005; Balestrini et al, 2007; Tisserant et al, 2012; Fiorilli et al, 2013; Walder et al, 2016). Fiorilli et al (2013) showed a slight down-regulation of RiPT1 when exposed to higher P concentrations, and Walder et al (2016) reported a positive correlation of RiPT5 transcript levels and Pi acquisition in Sorghum.…”

Section: Discussionmentioning

confidence: 99%

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A Functional Approach towards Understanding the Role of the Mitochondrial Respiratory Chain in an Endomycorrhizal Symbiosis

Mercy¹,

Lucic-Mercy²,

Nogales

et al. 2017

Front. Plant Sci.

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Arbuscular mycorrhizal fungi (AMF) are crucial components of fertile soils, able to provide several ecosystem services for crop production. Current economic, social and legislative contexts should drive the so-called “second green revolution” by better exploiting these beneficial microorganisms. Many challenges still need to be overcome to better understand the mycorrhizal symbiosis, among which (i) the biotrophic nature of AMF, constraining their production, while (ii) phosphate acts as a limiting factor for the optimal mycorrhizal inoculum application and effectiveness. Organism fitness and adaptation to the changing environment can be driven by the modulation of mitochondrial respiratory chain, strongly connected to the phosphorus processing. Nevertheless, the role of the respiratory function in mycorrhiza remains largely unexplored. We hypothesized that the two mitochondrial respiratory chain components, alternative oxidase (AOX) and cytochrome oxidase (COX), are involved in specific mycorrhizal behavior. For this, a complex approach was developed. At the pre-symbiotic phase (axenic conditions), we studied phenotypic responses of Rhizoglomus irregulare spores with two AOX and COX inhibitors [respectively, salicylhydroxamic acid (SHAM) and potassium cyanide (KCN)] and two growth regulators (abscisic acid – ABA and gibberellic acid – Ga3). At the symbiotic phase, we analyzed phenotypic and transcriptomic (genes involved in respiration, transport, and fermentation) responses in Solanum tuberosum/Rhizoglomus irregulare biosystem (glasshouse conditions): we monitored the effects driven by ABA, and explored the modulations induced by SHAM and KCN under five phosphorus concentrations. KCN and SHAM inhibited in vitro spore germination while ABA and Ga3 induced differential spore germination and hyphal patterns. ABA promoted mycorrhizal colonization, strong arbuscule intensity and positive mycorrhizal growth dependency (MGD). In ABA treated plants, R. irregulare induced down-regulation of StAOX gene isoforms and up-regulation of genes involved in plant COX pathway. In all phosphorus (P) concentrations, blocking AOX or COX induced opposite mycorrhizal patterns in planta: KCN induced higher Arum-type arbuscule density, positive MGD but lower root colonization compared to SHAM, which favored Paris-type formation and negative MGD. Following our results and current state-of-the-art knowledge, we discuss metabolic functions linked to respiration that may occur within mycorrhizal behavior. We highlight potential connections between AOX pathways and fermentation, and we propose new research and mycorrhizal application perspectives.

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Biotic Influences: Symbiotic Associations

Lambers

Oliveira

2019

Plant Physiological Ecology

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Regulation of plants' phosphate uptake in common mycorrhizal networks: Role of intraradical fungal phosphate transporters

Cited by 44 publications

References 19 publications

Nicotiana attenuata's capacity to interact with arbuscular mycorrhiza alters its competitive ability and elicits major changes in the leaf transcriptome

Nicotiana attenuata's capacity to interact with arbuscular mycorrhiza alters its competitive ability and elicits major changes in the leaf transcriptome

A Functional Approach towards Understanding the Role of the Mitochondrial Respiratory Chain in an Endomycorrhizal Symbiosis

Biotic Influences: Symbiotic Associations

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