Regulation of photosynthetic carbon metabolism in aquatic and terrestrial organisms by Rubisco activase, redox-modulation and CP12

Gontero, Brigitte; Salvucci, Michael E.

doi:10.1016/j.aquabot.2014.05.011

Cited by 20 publications

(23 citation statements)

References 119 publications

(148 reference statements)

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“…[39][40][41][42] Yruela and Contreras-Moreira (2012) analyzed 12 plants genomes and concluded that the nuclear-encoded proteins follow the same trend that other multi-cellular eukaryotes and show a higher disorder in the internal part of nuclear-encoded proteomes rather than at their extremities, in contrast to the chloroplast-and mitochondrion-encoded proteomes. 43 RuBisCO activase (RCA) might be present in 2 different isoforms: a-isoform and b-isoform in photosynthetic organism, 44 but not all species appear to have both isoforms (e.g., Nicotiana tabacum has one RCA isoform). [45][46][47] Fig.…”

Section: Rubisco and Rubisco Activase (Rca)mentioning

confidence: 99%

Comparison of the intrinsic disorder propensities of the RuBisCO activase enzyme from the motile and non-motile oceanic green microalgae

Sena

Uversky

2016

Intrinsically Disordered Proteins

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Green oceanic microalgae are efficient converters of solar energy into the biomass via the photosynthesis process, with the first step of carbon fixation in the photosynthesis being controlled by the enzyme ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO), which is a large proteinaceous machine composed of large (L, 52 kDa) and small (S, 12 kDa) subunits arranged as a L 8 S 8 hexadecamer that catalyzes the formation of 2 phosphoglyceric acid molecules from one ribulose 1,5-bisphosphate (RuBP) molecule and one of carbon dioxide (CO 2 ) and that is considered as the most abundant protein on Earth. The catalytic efficiency of this protein is controlled by the RuBisCO activase (RCA) that interacts with RuBisCO and promotes the CO 2 entrance to the active site of RuBisCO by removing RuBP. One of the peculiar features of RCA is the presence of functional disordered tails that might play a role in RCA-RuBisCO interaction. Based on their ability to move, microalgae are grouped into 2 major class, motile and non-motile. Motile microalgae have an obvious advantage over their non-motile counterparts because of their ability to actively migrate within the water column to find the most optimal environmental conditions. We hypothesizes that the RCA could be functionally different in the non-motile and motile microalgae. To check this hypothesis, we conducted a comparative computational analysis of the RCAs from the representatives of the non-motile (Ostreococcus tauri) and motile (Tetraselmis sp. GSL018) green oceanic microalgae.

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Section: Rubisco and Rubisco Activase (Rca)mentioning

confidence: 99%

Comparison of the intrinsic disorder propensities of the RuBisCO activase enzyme from the motile and non-motile oceanic green microalgae

Sena

Uversky

2016

Intrinsically Disordered Proteins

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“…Regulation of Rubisco activity has not been studied in algae, although Gontero et al (2014) speculate that it is probably similar to plants (Gontero et al 2014). Importantly, deactivation of photosynthetic enzymes is thought to be mediated by oxidized thioredoxins, molecular oxygen and accumulation of oxidants (Schürmann andBuchanan, 2008, Dangoor et al, 2012).…”

Section: Redox Regulation Of Photosynthetic Enzyme Activitymentioning

confidence: 99%

“…There is a lack of data on carboxylation activity in algae (Gontero and Salvucci, 2014). However notable studies have shown dramatic changes of Pmax or µmax under varying steady state irradiance levels as well as to changes in culture density.…”

Section: Predicted Changes In Pmaxmentioning

confidence: 99%

“…It is also recommended for studies to investigate the regulation of Rubisco activity, particularly in the context of fluctuating light and for algae, which at present, seems to be a critical gap in knowledge (Gontero and Salvucci, 2014).…”

Section: Opportunities and Future Directionsmentioning

confidence: 99%

“…Since LHCSR3 is found in other Chlorophyta, including one of the earliest lineages, Ostreococcus (Peers et al, 2009), this suggests that this qE mechanism is highly conserved and therefore applicable to many other green algae. In spite of reduced photoprotection, the batch cultivation in ePBRs ( decrease under higher cell densities due to reduced ATP production and oxidation of the ETC under light-to-dark transitions (Gontero et al 2014). This could lead to oxidation of Rubisco activase and consequentially, a higher proportion of deactivated Rubisco, thereby diminishing the capacity for carbon assimilation which will ultimately affect maximum photosynthetic and growth rates (Pmax and µmax respectively) -a factor that was revealed from a sensitivity analysis to have a high impact on productivity estimates (Chapter 2).…”

Section: Introductionmentioning

confidence: 99%

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Photosynthesis of microalgae in outdoor mass cultures and modelling its effects on biomass productivity for fuels, feeds and chemicals

Yarnold¹

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Practical applications of microalgae include uses as feedstocks for sustainable fuels, feeds, nutraceuticals, and chemicals; for the treatment of wastewater and industrial flue gas; and for use as recombinant protein expression systems. Improvements in biomass yields are required for commercially viable and sustainable algal technologies, particularly for low value commodities such as biofuels.Although several algae exhibit higher growth rates than terrestrial crops, with theoretical upper limits of 8-10% photon conversion efficiency (PCE), solar-illuminated outdoor microalgal production systems typically have a PCE far below this and even below those achieved under laboratory conditions. The most intractable limiting factor is poor light distribution through the mass culture, where photoinhibitory light at the surface and dark zones deeper in the culture reduce photosynthetic productivity. Moreover, microalgae have evolved a suite of photoacclimation and photoregulation mechanisms to cope with rapid (seconds to minutes) light fluxes in natural environments, yet these processes remain poorly understood in well-mixed photobioreactors.The aim of this thesis study was to combine an experimental and theoretical approach to: 1) investigate and understand the photosynthetic response of algae under mass culture conditions; 2) develop a mathematical model which could accurately predict light-tobiomass productivities under a broad range of design scenarios for outdoor production systems; and 3) identify key biological and design parameters to maximise biomass yields.In the first part of the study, a light-to-biomass model was developed that could be easily applied to rapidly assess biomass yields of different algal strains under a range of design scenarios. This model predicts temporal and spatial irradiance at the reactor surface and through the mass culture with inputs of local solar radiation data, local coordinates, system properties and the optical properties of the culture. Local growth rates are then coupled to local light intensities within the reactor based on a static Haldane growth model derived from empirical growth-irradiance curves. Growth rates are integrated over space and time to compute areal and volumetric productivities.Validation of the model was attempted by conducting batch harvest experiments in a laboratory scale photobioreactor matrix which simulated diurnal light cycles representative of three 'typical' days of solar radiation in Brisbane. The strains used were ii Chlamydomonas reinhardtii and its truncated light-harvesting antenna mutant, tla1, reported to possess improved optical properties. Initially, the model did not satisfactorily predict growth under batch cultivation for the three different incident light conditions, overestimating productivity under low light days and overestimating on high light days.Subsequent adjustment of the model to include photoacclimative changes in the optical properties of the cell and a re-fit of the growth parameter values provided a more accurate m...

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An Introduction to Productivity and Carbon Cycling in Aquatic Ecosystems

Gontero¹,

Lenton²,

Maberly³

2022

Blue Planet, Red and Green Photosynthesis

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Regulation of photosynthetic carbon metabolism in aquatic and terrestrial organisms by Rubisco activase, redox-modulation and CP12

Cited by 20 publications

References 119 publications

Comparison of the intrinsic disorder propensities of the RuBisCO activase enzyme from the motile and non-motile oceanic green microalgae

Comparison of the intrinsic disorder propensities of the RuBisCO activase enzyme from the motile and non-motile oceanic green microalgae

Photosynthesis of microalgae in outdoor mass cultures and modelling its effects on biomass productivity for fuels, feeds and chemicals

An Introduction to Productivity and Carbon Cycling in Aquatic Ecosystems

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