Regulation of phospholipid biosynthesis in Saccharomyces cerevisiae by inositol. Inositol is an inhibitor of phosphatidylserine synthase activity.

Kelley, Michael J.; Bailis, Adam M.; Henry, Susan A.; Carman, George M.

doi:10.1016/s0021-9258(19)81325-x

Cited by 143 publications

(14 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…71 Nevertheless, supplementation of yeast cells with inositol increases the cellular level of PtdIns, because the K m of Pis1p for inositol is much higher than the intracellular inositol concentration. 116 The increase of PtdIns occurs at the expense of PtdSer because both PtdIns synthase (Pis1p) and PtdSer synthase (Pss1p/Cho1p) compete for CDP-DAG, the common precursor.…”

Section: Phospholipid Synthesismentioning

confidence: 99%

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Daum

Lees

Bard

et al. 1998

Yeast

579

380

View full text Add to dashboard Cite

The yeast Saccharomyces cerevisiae is a powerful experimental system to study biochemical, cell biological and molecular biological aspects of lipid synthesis. Most but not all genes encoding enzymes involved in fatty acid, phospholipid, sterol or sphingolipid biosynthesis of this unicellular eukaryote have been cloned, and many gene products have been functionally characterized. Less information is available about genes and gene products governing the transport of lipids between organelles and within membranes, turnover and degradation of complex lipids, regulation of lipid biosynthesis, and linkage of lipid metabolism to other cellular processes. Here we summarize current knowledge about lipid biosynthetic pathways in S. cerevisiae and describe the characteristic features of the gene products involved. We focus on recent discoveries in these fields and address questions on the regulation of lipid synthesis, subcellular localization of lipid biosynthetic steps, cross‐talk between organelles during lipid synthesis and subcellular distribution of lipids. Finally, we discuss distinct functions of certain key lipids and their possible roles in cellular processes. © 1998 John Wiley & Sons, Ltd.

show abstract

Section: Phospholipid Synthesismentioning

confidence: 99%

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Daum

Lees

Bard

et al. 1998

Yeast

579

380

View full text Add to dashboard Cite

show abstract

“…The transcription of genes in this pathway is tightly governed by a number of key regulators. One of these primary regulators is inositol, a crucial phospholipid precursor that plays a key role in regulating phospholipid metabolism based on its availability [69][70][71][72]. When present in the growth medium, inositol is not required to be synthesized by the cell and thus the genes involved in its production are turned off.…”

Section: Regulation Of the Lipid Biosynthetic Pathwaymentioning

confidence: 99%

“…As explained earlier, inositol is one of the key phospholipid precursors whose presence is essential in regulating phospholipid metabolism [69][70][71][72]. When present in the growth medium, inositol production is repressed.…”

Section: V-atpase Genes and The Lipid Biosynthetic Pathwaymentioning

confidence: 99%

The Interplay of Key Phospholipid Biosynthetic Enzymes and the Yeast V-ATPase Pump and their Role in Programmed Cell Death

Sherr¹,

Shen²

2022

Regulation and Dysfunction of Apoptosis

View full text Add to dashboard Cite

Exposure of the yeast Saccharomyces cerevisiae to environmental stress can influence cell growth, physiology and differentiation, and thus result in a cell’s adaptive response. During the course of an adaptive response, the yeast vacuoles play an important role in protecting cells from stress. Vacuoles are dynamic organelles that are similar to lysosomes in mammalian cells. The defect of a lysosome’s function may cause various genetic and neurodegenerative diseases. The multi-subunit V-ATPase is the main regulator for vacuolar function and its activity plays a significant role in maintaining pH homeostasis. The V-ATPase is an ATP-driven proton pump which is required for vacuolar acidification. It has also been demonstrated that phospholipid biosynthetic genes might influence vacuolar morphology and function. However, the mechanistic link between phospholipid biosynthetic genes and vacuolar function has not been established. Recent studies have demonstrated that there is a regulatory role of Pah1p, a phospholipid biosynthetic gene, in V-ATPase disassembly and activity. Therefore, in this chapter we will use Saccharomyces cerevisiae as a model to discuss how Pah1p affects V-ATPase disassembly and activity and how Pah1p negatively affect vacuolar function. Furthermore, we propose a hypothesis to describe how Pah1p influences vacuolar function and programmed cell death through the regulation of V-ATPase.

show abstract

“…This was the first eukaryotic phospholipid synthesis gene cloned and the second eukaryotic phospholipid synthesis enzyme purified, the first being the yeast PI synthase ( 42 ). Susan and I have published numerous papers ( 43 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ) and reviews ( 59 , 60 , 61 , 62 , 63 ) on the regulation of phospholipid synthesis in yeast with a focus on the water-soluble phospholipid precursors inositol and choline. Central to this work is the Opi1/Ino2-Ino4 (Henry) regulatory circuit discovered in Susan’s laboratory ( 60 , 64 , 65 , 66 ).…”

Section: The Research That Shaped My Careermentioning

confidence: 99%

“…4 ), with whom we published numerous papers. Susan Henry and I have collaborated in many studies on the transcriptional regulation of phospholipid synthesis ( 43 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ). I have learned much from her amazing insight into how phospholipid synthesis is regulated.…”

Section: The Mentors Mentees and Collaborators That Helped Shape My Careermentioning

confidence: 99%

Lipid metabolism has been good to me

Carman

2021

Journal of Biological Chemistry

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Regulation of phospholipid biosynthesis in Saccharomyces cerevisiae by inositol. Inositol is an inhibitor of phosphatidylserine synthase activity.

Cited by 143 publications

References 51 publications

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

The Interplay of Key Phospholipid Biosynthetic Enzymes and the Yeast V-ATPase Pump and their Role in Programmed Cell Death

Lipid metabolism has been good to me

Contact Info

Product

Resources

About