1989
DOI: 10.1002/jlb.45.3.239
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Regulation of Phagolysosome pH in Bovine and Human Neutrophils: The Role of NADPH Oxidase Activity and an Na+/H+ Antiporter

Abstract: Fluorescein-labelled Staphylococcus aureus were used to follow changes in phagolysosome (PL) pH of bovine and human neutrophils following phagocytosis. Under aerobic conditions there was an alkalinisation of the PL followed by a slow decline. Under anaerobic conditions no alkalinisation of the PL was seen, and pharmacological inhibition of the NADPH oxidase with diphenyleneiodonium (DPI) resulted in a rapid acidification of the PL following phagocytosis. The inclusion of amiloride, an inhibitor of Na+/H+ antip… Show more

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Cited by 24 publications
(21 citation statements)
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“…Accordingly, the contribution of neutrophil enzymes (e.g., activity of NOX, MPO etc.) to the viability is critical (Mayer et al, 1989;Jankowski et al, 2002). This supports the assumption of the existence of a good correlation between PMN viability and CL.…”
Section: Wwwintechopencomsupporting
confidence: 79%
“…Accordingly, the contribution of neutrophil enzymes (e.g., activity of NOX, MPO etc.) to the viability is critical (Mayer et al, 1989;Jankowski et al, 2002). This supports the assumption of the existence of a good correlation between PMN viability and CL.…”
Section: Wwwintechopencomsupporting
confidence: 79%
“…There are, however, some possible explanations for this discrepancy. According to studies on human and bovine PMN (Mayer et al, 1989;Jankowski et al, 2002), PMN NADPH-oxidase activity (an index of PMAinduced PMN CL) and their viability are interrelated. PMA is a potent NADPH-oxidase and protein kinase-C agonist (Karlsson et al, 2000).…”
Section: Discussionmentioning
confidence: 99%
“…As the NADPH-oxidase is a trigger of PMN respiratory burst and proton transportation into the PMN phagosomal and cytosolic space, the enzyme's activity is regulated by MPO as well (Edwards and Swan, 1986 MPO activity impairment. The contribution of neutrophil NADPH-oxidase activity is pivotal for their viability (Mayer et al, 1989;Jankowski et al, 2002). Furthermore, NADPH-oxidase activity contributes to phagosomal and cytosolic pH homeostasis (Reeves et al, 2002), maintaining PMN stoichiometry and electroneutrality (Takanaka and O'Brien, 1988).…”
Section: Discussionmentioning
confidence: 99%
“…The anionic charge is compensated by a K + flux from cytosol into the phagosome [194]. The large amount of K + in the phagosome is a crucial factor for the non-oxidative bactericidal activity by its effect on proteases and pH homeostasis [111,153,194].…”
Section: Non-oxidative Mechanisms Of E Coli Killingmentioning
confidence: 99%