Regulation of Phagolysosome pH in Bovine and Human Neutrophils: The Role of NADPH Oxidase Activity and an Na+/H+ Antiporter

Mayer, S.; Keen, P.; Craven, N.; Bourne, F.J.

doi:10.1002/jlb.45.3.239

Cited by 24 publications

(21 citation statements)

References 32 publications

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“…Accordingly, the contribution of neutrophil enzymes (e.g., activity of NOX, MPO etc.) to the viability is critical (Mayer et al, 1989;Jankowski et al, 2002). This supports the assumption of the existence of a good correlation between PMN viability and CL.…”

Section: Wwwintechopencomsupporting

confidence: 79%

Molecular Aspects of Neutrophils as Pivotal Circulating Cellular Innate Immune Systems to Protect Mammary Gland from Pathogens

Mehrzad¹

2012

Recent Advances in Immunology to Target Cancer, Inflammation and Infections

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Section: Wwwintechopencomsupporting

confidence: 79%

Molecular Aspects of Neutrophils as Pivotal Circulating Cellular Innate Immune Systems to Protect Mammary Gland from Pathogens

Mehrzad¹

2012

Recent Advances in Immunology to Target Cancer, Inflammation and Infections

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“…There are, however, some possible explanations for this discrepancy. According to studies on human and bovine PMN (Mayer et al, 1989;Jankowski et al, 2002), PMN NADPH-oxidase activity (an index of PMAinduced PMN CL) and their viability are interrelated. PMA is a potent NADPH-oxidase and protein kinase-C agonist (Karlsson et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…As the NADPH-oxidase is a trigger of PMN respiratory burst and proton transportation into the PMN phagosomal and cytosolic space, the enzyme's activity is regulated by MPO as well (Edwards and Swan, 1986 MPO activity impairment. The contribution of neutrophil NADPH-oxidase activity is pivotal for their viability (Mayer et al, 1989;Jankowski et al, 2002). Furthermore, NADPH-oxidase activity contributes to phagosomal and cytosolic pH homeostasis (Reeves et al, 2002), maintaining PMN stoichiometry and electroneutrality (Takanaka and O'Brien, 1988).…”

Section: Discussionmentioning

confidence: 99%

Blood and Milk Neutrophil Chemiluminescence and Viability in Primiparous and Pluriparous Dairy Cows During Late Pregnancy, Around Parturition and Early Lactation

Mehrzad

Duchateau

Pyörälä

et al. 2002

Journal of Dairy Science

111

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Extensive studies have shown the polymorphonuclear leukocytes (PMN) dysfunction inextricably links to parturition. To investigate the effect of parity on PMN function, phorbol 12-myristate 13-acetate (PMA) stimulated luminol-amplified chemiluminescence (CL) and viability of blood and milk PMN were investigated in primiparous and pluriparous dairy cows during periparturient period. The CL kinetics of blood and milk PMN and hematological profiles were also assessed. Milk PMN CL was always lower than blood PMN CL. Blood and milk PMN CL and milk PMN viability were significantly higher in primiparous cows throughout the study. Blood PMN CL in pluriparous cows showed a sharper decrease. Both in pluriparous and in primiparous cows, minimal blood PMN CL appeared at periparturient day (PPD) 2. After PPD 7, blood PMN CL recovery rate was faster in primiparous cows. Milk PMN CL was minimal at PPD 2 in both groups. Whereas no changes were observed in blood PMN viability, the viability of milk PMN in primiparous cows was substantially higher than in pluriparous cows. The number of circulating eosinophils and immature neutrophils was substantially higher in primiparous cows throughout the study. The CL kinetics of blood PMN at PPD −2 and 2 and of milk PMN at PPD 2 exhibited different responses to PMA, with higher intensity and durability, peaking and subsiding more slowly in primiparous dairy cows. The pronounced reduction in PMN CL and viability in milk PMN of pluriparous cows may be involved in the underlying mechanisms that Received April 19, 2002. Accepted July 18, 2002 Corresponding author: C. Burvenich; e-mail: christian.burvenich @rug.ac.be. 3268make these animals more susceptible to periparturient infectious diseases. (Key words: chemiluminescence, neutrophil, periparturient, pluriparous, primiparous) Abbreviation key: AUC = area under the curve, CL = chemiluminescence, MPO = myeloperoxidase, PMA = phorbol 12-myristate 13-acetate, PMN = polymorphonuclear leukocyte, PPD = periparturient day, ROS = reactive oxygen species.

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“…The anionic charge is compensated by a K + flux from cytosol into the phagosome [194]. The large amount of K + in the phagosome is a crucial factor for the non-oxidative bactericidal activity by its effect on proteases and pH homeostasis [111,153,194].…”

Section: Non-oxidative Mechanisms Of E Coli Killingmentioning

confidence: 99%

Severity of E. coli mastitis is mainly determined by cow factors

et al. 2003

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-Intramammary infections of dairy cows with Gram-positive bacteria such as Staphylococcus aureus (major cause of mastitis) have received a lot of attention because of their major economic impact on the dairy farm through production losses induced by an increase in somatic cell count. Management strategies, including greater awareness for efficient milking and hygienic measures, have limited the spread of Gram-positive bacteria and resulted in a significant decrease of proportion of S. aureus isolates and subclinical mastitis worldwide. Other organisms such as coliform subspecies and Streptococcus uberis, both environmental bacteria that cause clinical mastitis, have received less attention. Escherichia coli causes inflammation of the mammary gland in dairy cows around parturition and during early lactation with striking local and sometimes severe systemic clinical symptoms. This disease affects many high producing cows in dairy herds and may cause several cases of death per year in the most severe cases. It is well known that bacterial, cow and environmental factors are interdependent and influence mastitis susceptibility. Many studies, executed during the last decade, indicate that the severity of E. coli mastitis is mainly determined by cow factors rather than by E. coli pathogenicity. During E. coli mastitis, the host defense status is a cardinal factor determining the outcome of the disease. Today, we know that the neutrophil is a key factor in the cows' defense against intramammary infection with E. coli. Effective elimination of the pathogen by neutrophils is important for the resolution of infection and the outcome of E. coli mastitis. This review is a compilation of some major findings over the last 15 years concerning mainly host factors that modulate and influence neutrophil function and the mammary inflammatory reaction. The individual chapters address: virulence factors of E. coli strains, how neutrophils kill E. coli, connection between endotoxins, tumor necrosis factor-a and nitric oxide, severity classification of E. coli mastitis, lifespan of neutrophils, host factors that influence severity, tissue damage and production loss.

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Regulation of Phagolysosome pH in Bovine and Human Neutrophils: The Role of NADPH Oxidase Activity and an Na+/H+ Antiporter

Cited by 24 publications

References 32 publications

Molecular Aspects of Neutrophils as Pivotal Circulating Cellular Innate Immune Systems to Protect Mammary Gland from Pathogens

Molecular Aspects of Neutrophils as Pivotal Circulating Cellular Innate Immune Systems to Protect Mammary Gland from Pathogens

Blood and Milk Neutrophil Chemiluminescence and Viability in Primiparous and Pluriparous Dairy Cows During Late Pregnancy, Around Parturition and Early Lactation

Severity of E. coli mastitis is mainly determined by cow factors

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