Regulation of Peripheral Myelination through Transcriptional Buffering of Egr2 by an Antisense Long Non-coding RNA

Martinez-Moreno, Margot; O’Shea, Timothy M.; Zepecki, John P.; Olaru, Alexander; Ness, Jennifer K.; Langer, Robert; Tapinos, Nikos

doi:10.1016/j.celrep.2017.07.068

Cited by 33 publications

(28 citation statements)

References 57 publications

(73 reference statements)

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“…Antisense lncRNAs are known to regulate the transcription output RNA in mammals, affecting the mRNA expression [39,40] and stabilizing the mRNA [40]. Some antisense lncRNAs may act on their neighboring coding genes, reducing the complementary mRNA expression [41,42], while others clearly upregulate the expression of the corresponding mRNA and protein [43].…”

Section: Resultsmentioning

confidence: 99%

Machine Learning Supports Long Noncoding RNAs as Expression Markers for Endometrial Carcinoma

Mello

Freitas

Coutinho

et al. 2020

BioMed Research International

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Uterine corpus endometrial carcinoma (UCEC) is the second most common type of gynecological tumor. Several research studies have recently shown the potential of different ncRNAs as biomarkers for prognostics and diagnosis in different types of cancers, including UCEC. Thus, we hypothesized that long noncoding RNAs (lncRNAs) could serve as efficient factors to discriminate solid primary (TP) and normal adjacent (NT) tissues in UCEC with high accuracy. We performed an in silico differential expression analysis comparing TP and NT from a set of samples downloaded from the Cancer Genome Atlas (TCGA) database, targeting highly differentially expressed lncRNAs that could potentially serve as gene expression markers. All analyses were performed in R software. The receiver operator characteristics (ROC) analyses and both supervised and unsupervised machine learning indicated a set of 14 lncRNAs that may serve as biomarkers for UCEC. Functions and putative pathways were assessed through a coexpression network and target enrichment analysis.

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Section: Resultsmentioning

confidence: 99%

Machine Learning Supports Long Noncoding RNAs as Expression Markers for Endometrial Carcinoma

Mello

Freitas

Coutinho

et al. 2020

BioMed Research International

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“…Most of these studies combine lncRNA and mRNA expression analyses including complex bioinformatics to generate networks of potential functional interactions [180,186,187]; however, only few have been explored using a more mechanistic approach ( Table 5). Differential expression of lncRNAs upon peripheral nerve injuries [199] Abbreviations: CCI, chronic constriction injury; CCI-ION, chronic constriction injury of the infraorbital nerve; CFA, Complete Freund's adjuvant; CRPS, chronic regional pain syndrome; DPN, diabetic peripheral neuropathy; DRG, dorsal root ganglia; LucA, luciferase assay; RIP, RNA immunoprecipitation; SCI, spinal cord injury; SNL, sciatic nerve ligation; WB, Western blot.…”

Section: Lncrnas As Possible Signatures For Pain Disordersmentioning

confidence: 99%

“…This model is used to explore nerve regeneration and numerous differentially expressed lncRNAs were associated with inflammatory and immune responses, which could possibly be relevant for neuropathic pain [185]. Particularly, NONMMUG014387 enhances the proliferation of cultured mouse Schwann cells, presumably via cis-upregulation of collagene triple helix repeat containing 1 (Cthrc1) [185], whereas the antisense to the Egr2 promoter ncRNA (Egr2-antisense-RNA) down-regulates Egr2 and promotes demyelination upon peripheral nerve injury by an epigenetic mechanism, in which a chromatin remodeling complex is assembled at the Egr2 promoter [199].…”

Section: Lncrnas As Possible Signatures For Pain Disordersmentioning

confidence: 99%

Non-coding RNAs in neuropathic pain

2020

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Neuro-immune alterations in the peripheral and central nervous system play a role in the pathophysiology of chronic pain in general, and members of the non-coding RNA (ncRNA) family, specifically the short, 22 nucleotide microRNAs (miRNAs) and the long non-coding RNAs (lncRNAs) act as master switches orchestrating both immune as well as neuronal processes. Several chronic disorders reveal unique ncRNA expression signatures, which recently generated big hopes for new perspectives for the development of diagnostic applications. lncRNAs may offer perspectives as candidates indicative of neuropathic pain in liquid biopsies. Numerous studies have provided novel mechanistic insight into the role of miRNAs in the molecular sequelae involved in the pathogenesis of neuropathic pain along the entire pain pathway. Specific processes within neurons, immune cells, and glia as the cellular components of the neuropathic pain triad and the communication paths between them are controlled by specific miRNAs. Therefore, nucleotide sequences mimicking or antagonizing miRNA actions can provide novel therapeutic strategies for pain treatment, provided their human homologues serve the same or similar functions. Increasing evidence also sheds light on the function of lncRNAs, which converge so far mainly on purinergic signalling pathways both in neurons and glia, and possibly even other ncRNA species that have not been explored so far.

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“…The demyelination of peripheral sensory fibers after injury participates in neuropathic pain [51]. An antisense lncRNA, which is transcribed for the opposite strand of the proximal promoter of Egr2 , was discovered by Martinez-Moreno et al in both mouse and rat sciatic nerves [52]. After peripheral nerve injury, the expression of Egr2 AS RNA is increased and affects the expression of Egr2 mRNA via an epigenetic mechanism [52].…”

Section: Lncrnas Expressed In Peripheral Nervesmentioning

confidence: 99%

Long noncoding RNA (lncRNA): a target in neuropathic pain

Bono

Tao

2018

Expert Opinion on Therapeutic Targets

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Introduction: Current treatments for neuropathic pain are limited at least in part due to the incomplete understanding of its underlying mechanisms. Recent evidence reveals the dysregulated expression of long non-coding RNAs (lncRNAs) in the damaged nerve, dorsal root ganglion (DRG), and spinal cord dorsal horn following peripheral nerve injury. However, the role of the majority of lncRNAs in neuropathic pain genesis is still elusive. Unveiling the mechanisms of how lncRNAs participate in neuropathic pain may develop new strategies to prevent and/or treat this disorder. Areas covered: This review focuses on the dysregulation of lncRNAs in the DRG, spinal cord dorsal horn, and the injured peripheral nerves from preclinical rodent models of neuropathic pain. We provide evidence of how peripheral nerve injury causes the dysregulation of lncRNAs in these pain-related regions. The potential mechanisms of how dysregulated lncRNAs contribute to the pathogenesis of neuropathic pain are discussed. Expert opinion: The investigation on the role of the dysregulated lncRNAs in neuropathic pain might open up a novel avenue for therapeutic treatment of this disorder. However, current investigation is at the infancy stage, which challenges the translation of preclinical findings. More intensive study on lncRNAs is required before the preclinical findings are translated into therapeutic management for neuropathic pain.

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Regulation of Peripheral Myelination through Transcriptional Buffering of Egr2 by an Antisense Long Non-coding RNA

Cited by 33 publications

References 57 publications

Machine Learning Supports Long Noncoding RNAs as Expression Markers for Endometrial Carcinoma

Machine Learning Supports Long Noncoding RNAs as Expression Markers for Endometrial Carcinoma

Non-coding RNAs in neuropathic pain

Long noncoding RNA (lncRNA): a target in neuropathic pain

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