Regulation of Pattern Formation and Gene Amplification During <i>Drosophila</i> Oogenesis by the miR-318 microRNA

Ge, Wanzhong; Deng, Qiannan; Guo, Ting; Hong, Xin; Kugler, Jan-Michael; Yang, Xiaohang; Cohen, Stephen M.

doi:10.1534/genetics.115.174748

Cited by 27 publications

(24 citation statements)

References 46 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies of various mutants that show defects in chorion gene amplification have revealed three different phenotypes. In addition to a lack of amplification (reviewed in Claycomb and Orr-Weaver, 2005), some mutants exhibit chorion gene overamplification (Royzman et al, 1999;Bosco et al, 2001), and other mutant follicle cells fail to exit the endocycle during the amplification stage and instead perform inappropriate genomic DNA replication throughout the follicle cell genome (Cayirlioglu et al, 2003;Aggarwal and Calvi, 2004;Beall et al, 2004;Ge et al, 2015). These results suggest that distinct signaling pathways exist for the positive and negative regulation of chorion gene amplification and for the repression of genomic DNA replication.…”

Section: Discussionmentioning

confidence: 99%

Initiation of Drosophila chorion gene amplification requires Claspin and mus101, whereas Claspin, but not mus101, plays a major role during elongation

Choi

Park

Nguyen

et al. 2017

Developmental Dynamics

View full text Add to dashboard Cite

Background: Claspin and TopBP1 are checkpoint mediators that are required for the phosphorylation of Chk1 by ATR to maintain genomic stability. Here, we investigated the functions of Drosophila Claspin and mus101 (TopBP1 ortholog) during chorion (eggshell component) gene amplification, which occurs in follicle cells in the absence of global genomic DNA replication. Results: Unlike Drosophila mei‐41 (ATR ortholog) mutant embryos, Claspin and mus101 mutant embryos showed severe eggshell defects resulting from defects in chorion gene amplification. EdU (5‐ethynyl‐2′‐deoxyuridine) incorporation assay during initiation and elongation stages revealed that Claspin and mus101 were required for initiation, while only Claspin had a major role in the efficient progression of the replication forks. Claspin proteins were enriched in the amplification foci both in the initiation and elongation stage‐follicle cell nuclei in a mei‐41‐independent manner. The focal localization of ORC2, a component of the origin recognition complex, was not significantly affected in the Claspin mutant, whereas it was reduced in the mus101 mutant. Conclusions: Drosophila Claspin plays a major role in the initiation and elongation stages of chorion gene amplification by localizing to the amplification foci in a mei‐41‐independent manner. Drosophila mus101 is also involved in chorion gene amplification, mostly functioning in initiation, rather than elongation. Developmental Dynamics 246:466–474, 2016. © 2017 The Authors Developmental Dynamics published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists

show abstract

Section: Discussionmentioning

confidence: 99%

Initiation of Drosophila chorion gene amplification requires Claspin and mus101, whereas Claspin, but not mus101, plays a major role during elongation

Choi

Park

Nguyen

et al. 2017

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…For fat‐body immunostaining, after fat‐body dissection, we used a staining protocol according to Ge et al (40). Primary Abs were rabbit anti‐PVR (1: 2000).…”

Section: Microscopy and Imagingmentioning

confidence: 99%

“…Western blot analysis of protein extracts from third‐instar larval fat bodies was performed as described (40). Rabbit anti‐PVR (1: 2000), mouse anti‐tubulin (1: 1000; DHSB E7), and mouse anti‐armadillo (1: 500; DSHB) were used as primary Abs.…”

Section: Western Blottingmentioning

confidence: 99%

“…Whole‐mount embryo in situ hybridization was performed as described (40). Alcohol dehydrogenase ( Adh ) probes were synthesized from the golden cline RH54514 by using a digoxigenin‐labeled uracil analog as described by the manufacturer (Boehringer, Mannheim, Germany).…”

Section: In Situ Hybridization To Whole‐mount Embryosmentioning

confidence: 99%

“…Total RNA was isolated from fat bodies with Trizol reagent (Thermo Fisher Scientific). cDNA was synthesized from RNA samples by using a First-Strand cDNA synthesis kit (Thermo In situ hybridization to whole-mount embryos Whole-mount embryo in situ hybridization was performed as described (40). Alcohol dehydrogenase (Adh) probes were synthesized from the golden cline RH54514 by using a digoxigenin-labeled uracil analog as described by the manufacturer (Boehringer, Mannheim, Germany).…”

Section: Rna Isolation and Cdna Synthesismentioning

confidence: 99%

See 2 more Smart Citations

Premature remodeling of fat body and fat mobilization triggered by platelet‐derived growth factor/VEGF receptor inDrosophila

et al. 2017

Self Cite

View full text Add to dashboard Cite

In fat-body remodeling accompanied with fat mobilization is an ecdysone-induced dynamic process that only occurs during metamorphosis. Here, we show that the activated platelet-derived growth factor/VEGF receptor (PVR) is sufficient to induce shape changes in the fat body, from thin layers of tightly conjugated polygonal cells to clusters of disaggregated round-shaped cells. These morphologic changes are reminiscent of those seen during early pupation upon initiation of fat-body remodeling. Activation of PVR also triggers an early onset of lipolysis and mobilization of internal storage, as revealed by the appearance of small lipid droplets and up-regulated lipolysis-related genes. We found that PVR displays a dynamic expression pattern in the fat body and peaks at the larval-prepupal transition under the control of ecdysone signaling. Removal of PVR, although it does not prevent ecdysone-induced fat-body remodeling, causes ecdysone signaling to be up-regulated. Our data reveal that PVR is active in a dual-secured mechanism that involves an ecdysone-induced fat-body remodeling pathway and a reinforced PVR pathway for effective lipid mobilization. Ectopic expression of activated c-kit-the mouse homolog of PVR in the fat body-also results in a similar phenotype. This may suggest a novel function of c-kit as it relates to lipid metabolism in mammals.-Zheng, H., Wang, X., Guo, P., Ge, W., Yan, Q., Gao, W., Xi, Y., Yang, X. Premature remodeling of fat body and fat mobilization triggered by platelet-derived growth factor/VEGF receptor in.

show abstract

RNA sequencing identifies an ovary‐enriched microRNA, miR‐311‐3p, involved in ovarian development and fecundity by targeting EndophilinB1 in Bactrocera dorsalis

Zhang

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

Background The oriental fruit fly, Bactrocera dorsalis, is a highly invasive pest in East Asia and the Pacific. With the development of pesticides resistance, environment‐friendly pesticides are urgently needed. MicroRNAs (miRNAs) are critical regulators of numerous biological processes, including reproduction. Thus, it is significant to identify reproductive‐related miRNAs in this notorious pest to facilitate its control, such as RNAi‐based biopesticides targeting essential miRNAs. Results A high‐throughput sequencing was carried out to identify miRNAs involved in reproduction from the ovary and fat body at four developmental stages [1 day (d), 5, 9, and 13 days post‐eclosion] in female B. dorsalis. Results showed that 98 and 74 miRNAs were differentially expressed in ovary and fat body, respectively, during sexual maturation. Gene ontology analysis showed that target genes involved in oogenesis and lipid particle accounted for 33% and 15% of the total targets, respectively. Among these differentially expressed miRNAs, we found by qPCR that miR‐311‐3p was enriched in the ovary and down‐regulated during sexual maturation. Injection of agomir‐miR‐311‐3p resulted in arrested ovarian development, reduced egg deposition and progeny viability. Endophilin B1 was confirmed to be the target of miR‐311‐3p, via dual‐luciferase assay and expression profiling. Knockdown of Endophilin B1 resulted in reproductive defects similar to those caused by injection of miR‐311‐3p agomir. Thus, miR‐311‐3p might play a critical role in female reproduction by targeting Endophilin B1. Conclusion Our data not only provides knowledge on the abundance of reproductive‐related miRNAs and target genes, but also promotes new control strategies for this pest. © 2022 Society of Chemical Industry.

show abstract

Regulation of Pattern Formation and Gene Amplification During Drosophila Oogenesis by the miR-318 microRNA

Cited by 27 publications

References 46 publications

Initiation of Drosophila chorion gene amplification requires Claspin and mus101, whereas Claspin, but not mus101, plays a major role during elongation

Initiation of Drosophila chorion gene amplification requires Claspin and mus101, whereas Claspin, but not mus101, plays a major role during elongation

Premature remodeling of fat body and fat mobilization triggered by platelet‐derived growth factor/VEGF receptor inDrosophila

RNA sequencing identifies an ovary‐enriched microRNA, miR‐311‐3p, involved in ovarian development and fecundity by targeting EndophilinB1 in Bactrocera dorsalis

Contact Info

Product

Resources

About