Regulation of Osteogenesis and Survival within Bone Grafts to the Calvaria: The Effect of the Dura versus the Pericranium

Gosain, Arun K.; Gosain, Sankalp A.; Sweeney, Walter M.; Song, Lixiao; Amarante, Marco T. J.

doi:10.1097/prs.0b013e31821740cc

Cited by 43 publications

(32 citation statements)

References 26 publications

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“…2,[25][26] Exogenous growth factors (eg, BMP) applied to the dura may enhance this osteogenic potential, as does mechanical separation, or tenting membranes over the dura. 2,[28][29][30][31][32] However, the osteogenic role of dura and scalp periosteum has not been investigated during cranial distraction. Typically, the regenerate accounts for the majority of new bone growth, but we postulate that the subjacent osteogenic dura positively modulates the process.…”

Section: Discussionmentioning

confidence: 98%

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Evaluation of Cranial Bone Transport Distraction With and Without Adipose Grafting

Koch¹,

Yuhasz²,

Travieso³

et al. 2014

Journal of Craniofacial Surgery

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Transport distraction osteogenesis (DO) can be used to autologously reconstitute calvarial defects. The purpose of this study is to histomorphologically interrogate osteogenic formation during cranial transport distraction using a novel device. We also evaluate the effect of fat grafting on the regenerate and soft-tissue stability during distraction. This study was approved by Yale IACUC. Ten male New Zealand white rabbits (3 mo; 3.5 kg) were used (8 treatment, 2 control). A 16 × 16 mm defect was created abutted by a 10 × 16 mm transport disc. The device was fixated anterioposteriorly. Four animals were fat-grafted using 2 mL of subdermal intrascapular fat deposited along the distraction site. Latency (1 d), active distraction (12-14 d) (1.5 mm/d), and consolidation (4 wk) followed. Calcein and xylene orange fluorochromes were injected subcutaneously during and post-distraction to mark sites of bone formation. Following sacrifice, osteogenesis was assessed using microCT, histology, and fluorescence. Treatment animals demonstrated regenerate bone between distracted segments on microCT. MicroCT analysis of non-fat-grafted and fat-grafted animals revealed a mean density of 2271.95 mgHA/ccm and 2254.27 mgHA/ccm (P = 0.967), respectively, and defect bone versus total volume (BV/TV) of 0.0999 and 0.0766 (P = 0.5979), respectively. Controls had minimal reossification. Histologically, mean densities measured 43.63% and 8.19%, respectively. Fluorescence revealed ossification from the callus as well as from dura and periosteum in the cranial defect. Transport distraction is effective to reconstruct critically sized rabbit calvarial defects. Regenerate bone arises predominantly from the callus with contribution from surrounding dura and periosteum. Adipose grafting is well tolerated but does not enhance osseous regeneration.

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Section: Discussionmentioning

confidence: 98%

“…31,33 Our purpose to implement them in this study was to identify and distinguish bone formation from all potential sites (periosteum, dura, regenerate, native bone marrow). As expected, we found that the callus behind the trailing edge of the transport segment was the principal contributor to osteogenesis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Cranial Bone Transport Distraction With and Without Adipose Grafting

Koch¹,

Yuhasz²,

Travieso³

et al. 2014

Journal of Craniofacial Surgery

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“…This remained clinically irrelevant but seems to confirm that periosteal coverage might have a positive effect [2, 18]. We think that preservation of the periosteum during decompressive craniectomy could be advantageous for a future cranioplasty.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the use of strong and thick adult bone makes natural partial reabsorption unlikely or at least limits it. Finally, like all biomaterials, graft coverage by vital pericranium may play a role in decreasing the reabsorption risk [2, 18], though its efficacy remains unproven.…”

Section: Discussionmentioning

confidence: 99%

Transplant of Adult Bone for Reconstruction of a Large Post-Traumatic Cranial Defect in a Very Young Baby

2019

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Large cranial defects in very young patients are challenging. The ideal material for cranioplasty in this age group has not yet been identified. Cryopreserved autologous bone presents very high rates of failures, acrylic resins pose a number of compatibility problems, bioceramics may be contraindicated, and autografts may be not adequate for repair of large defects. We present an 18-month-old baby with a large post-traumatic cranial defect which was repaired by assembling a new bone flap on a sterile stereolithographic 3-D model. This customized newly assembled flap consisted of a scaffold of autologous bone (from vault duplication) sustaining 2 large grafts of homologous cadaveric bone. It was adequately modeled and contoured on the 3-D model using metallic plates and screws. Immediately after implantation on the skull, the metallic devices were progressively replaced by reabsorbable material, thus maintaining the previously obtained flap profile. In this paper we detail this original technique which was developed to manage this specific patient.

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“…Falhas ósseas induzidas na região da calvária sob a influência da dura-máter e periósteo também tendem a apresentar volume de osso neoformado significativamente maior do que falhas ósseas em outras regiões, como, por exemplo, a região maxilar e alveolar sem a influência do efeito osteoindutor da dura-máter. 77 Essas três variáveis relacionadas ao modelo animal podem interferir no sucesso de uma terapia de bioengenharia de tecidos ósseos. Portanto, a padronização das dimensões de uma falha óssea induzida, bem como da espécie e idade do modelo animal são essenciais para testar fontes promissoras de CTM e novos biomateriais carreadores.…”

Section: Conceito De Falha óSsea Críticaunclassified

Análise da neoformação óssea em transplantes de osso autólogo, osso bovino mineral e tricálcio fosfato com e sem o emprego de células-tronco mesenquimais humanas no reparo de falhas ósseas alveolares por meio de histomorfometria e imagens

Amaral¹

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Regulation of Osteogenesis and Survival within Bone Grafts to the Calvaria: The Effect of the Dura versus the Pericranium

Cited by 43 publications

References 26 publications

Evaluation of Cranial Bone Transport Distraction With and Without Adipose Grafting

Evaluation of Cranial Bone Transport Distraction With and Without Adipose Grafting

Transplant of Adult Bone for Reconstruction of a Large Post-Traumatic Cranial Defect in a Very Young Baby

Análise da neoformação óssea em transplantes de osso autólogo, osso bovino mineral e tricálcio fosfato com e sem o emprego de células-tronco mesenquimais humanas no reparo de falhas ósseas alveolares por meio de histomorfometria e imagens

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