Regulation of Osteoblast Differentiation and Iron Content in MC3T3-E1 Cells by Static Magnetic Field with Different Intensities

Yang, Juhua; Zhang, Jian; Ding, Chong; Dong, Di; Shang, Peng

doi:10.1007/s12011-017-1161-5

Cited by 66 publications

(60 citation statements)

References 58 publications

(64 reference statements)

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“…The results showed that tibial and femoral BMD and BMC was lower, and there were more deleterious effects on femoral microstructure of trabecular bone, including Tb.Th, Tb.N, BV/TV, and BS/BV, and greater negative effects on femoral biomechanical characteristics, such as the elastic modulus, stiffness, ultimate stress, and the toughness in reloading mice under the HyMF environment compared to the GMF condition. Furthermore, HyMF inhibited osteoblastic mineralization, and promoted osteoclastic differentiation and bone resorption in vitro . Herein, we analyzed the effects of HyMF on the number of osteoblasts and osteoclasts in the tibia of mice reloaded after HLU in vivo.…”

Section: Discussionsupporting

confidence: 86%

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Disorder of Iron Metabolism Inhibits the Recovery of Unloading-Induced Bone Loss in Hypomagnetic Field

Xue

Yang

Luo

et al. 2019

Journal of Bone and Mineral Research

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Exposure of humans and animals to microgravity in spaceflight results in various deleterious effects on bone health. In addition to microgravity, the hypomagnetic field (HyMF) is also an extreme environment in space, such as on the Moon and Mars; magnetic intensity is far weaker than the geomagnetic field (GMF) on Earth. Recently, we showed that HyMF promoted additional bone loss in hindlimb unloading–induced bone loss, and the underlying mechanism probably involved an increase of body iron storage. Numerous studies have indicated that bone loss induced by mechanical unloading can be largely restored after skeletal reloading in GMF conditions. However, it is unknown whether this bone deficit can return to a healthy state under HyMF condition. Therefore, the purpose of this study is to examine the effects of HyMF on the recovery of microgravity‐induced bone loss, and illustrates the changes of body iron storage in this process. Our results showed that there was lower bone mineral content (BMC) in the HyMF reloading group compared to the GMF reloading group. Reloaded mice in the HyMF condition had a worse microstructure of femur than in the GMF condition. Femoral mechanical properties, including elastic modulus, stiffness, and ultimate stress, were poorer and toughness was higher in the HyMF group compared with the GMF group. Simultaneously, more iron content in serum, the tibia, liver, and spleen was found under HyMF reloading than GMF reloading. The iron chelator deferoxamine mesylate (DFO) decreased the iron content in the bone, liver, and spleen, and significantly relieved unloading‐induced bone loss under HyMF reloading. These results showed that HyMF inhibits the recovery of microgravity‐induced bone loss, probably by suppressing the elevated iron levels’ return to physiological level. © 2019 American Society for Bone and Mineral Research.

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Section: Discussionsupporting

confidence: 86%

“…However, our recent research indicated that the effects of HyMF on bone cannot be overlooked. In vitro, we found that HyMF can inhibit osteoblast differentiation and mineralization, and promote osteoclast formation and bone resorption . In vivo, HyMF aggravated bone loss induced by hindlimb unloading (HLU) in rats and mice .…”

Section: Introductionmentioning

confidence: 89%

Disorder of Iron Metabolism Inhibits the Recovery of Unloading-Induced Bone Loss in Hypomagnetic Field

Xue

Yang

Luo

et al. 2019

Journal of Bone and Mineral Research

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“…The product was quantified using a standard curve, and levels of gene expression were normalized to glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH). Relative gene expression was analyzed by the 2 −ΔΔCt method …”

Section: Methodsmentioning

confidence: 99%

2D MXene‐Integrated 3D‐Printing Scaffolds for Augmented Osteosarcoma Phototherapy and Accelerated Tissue Reconstruction

et al. 2019

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The residual of malignant tumor cells and lack of bone‐tissue integration are the two critical concerns of bone‐tumor recurrence and surgical failure. In this work, the rational integration of 2D Ti3C2 MXene is reported with 3D‐printing bioactive glass (BG) scaffolds for achieving concurrent bone‐tumor killing by photonic hyperthermia and bone‐tissue regeneration by bioactive scaffolds. The designed composite scaffolds take the unique feature of high photothermal conversion of integrated 2D Ti3C2 MXene for inducing bone‐tumor ablation by near infrared‐triggered photothermal hyperthermia, which has achieved the complete tumor eradication on in vivo bone‐tumor xenografts. Importantly, the rational integration of 2D Ti3C2 MXene is demonstrated to efficiently accelerate the in vivo growth of newborn bone tissue of the composite BG scaffolds. The dual functionality of bone‐tumor killing and bone‐tissue regeneration makes these Ti3C2 MXene‐integrated composite scaffolds highly promising for the treatment of bone tumors, which also substantially broadens the biomedical applications of 2D MXenes in tissue engineering, especially on the treatment of bone tumors.

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“…Fe(III) for example, participate in many biological aspects of cells being part of proteins and enzymes. It was shown that MF with different strengths modulates iron content in cells and thus contributes to enhanced osteoblasts differentiation [40]. What is worth noting, IOs and MF elevated the expression of OPN which is a crucial regulator of bone formation under mechanical stress.…”

Section: Discussionmentioning

confidence: 99%

Iron oxides nanoparticles (IOs) exposed to magnetic field promote expression of osteogenic markers in osteoblasts through integrin alpha-3 (INTa-3) activation, inhibits osteoclasts activity and exerts anti-inflammatory action

Marycz¹,

Sobierajska²,

Roecken³

et al. 2020

J Nanobiotechnol

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Background: Prevalence of osteoporosis is rapidly growing and so searching for novel therapeutics. Yet, there is no drug on the market available to modulate osteoclasts and osteoblasts activity simultaneously. Thus in presented research we decided to fabricate nanocomposite able to: (i) enhance osteogenic differentiation of osteoblast, (i) reduce osteoclasts activity and (iii) reduce pro-inflammatory microenvironment. As a consequence we expect that fabricated material will be able to inhibit bone loss during osteoporosis. Results:The α-Fe 2 O 3 /γ-Fe 2 O 3 nanocomposite (IOs) was prepared using the modified sol-gel method. The structural properties, size, morphology and Zeta-potential of the particles were studied by means of XRPD (X-ray powder diffraction), SEM (Scanning Electron Microscopy), PALS and DLS techniques. The identification of both phases was checked by the use of Raman spectroscopy and Mössbauer measurement. Moreover, the magnetic properties of the obtained IOs nanoparticles were determined. Then biological properties of material were investigated with osteoblast (MC3T3), osteoclasts (4B12) and macrophages (RAW 264.7) in the presence or absence of magnetic field, using confocal microscope, RT-qPCR, western blot and cell analyser. Here we have found that fabricated IOs: (i) do not elicit immune response; (ii) reduce inflammation; (iii) enhance osteogenic differentiation of osteoblasts; (iv) modulates integrin expression and (v) triggers apoptosis of osteoclasts. Conclusion:Fabricated by our group α-Fe 2 O 3 /γ-Fe 2 O 3 nanocomposite may become an justified and effective therapeutic intervention during osteoporosis treatment.

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Regulation of Osteoblast Differentiation and Iron Content in MC3T3-E1 Cells by Static Magnetic Field with Different Intensities

Cited by 66 publications

References 58 publications

Disorder of Iron Metabolism Inhibits the Recovery of Unloading-Induced Bone Loss in Hypomagnetic Field

Disorder of Iron Metabolism Inhibits the Recovery of Unloading-Induced Bone Loss in Hypomagnetic Field

2D MXene‐Integrated 3D‐Printing Scaffolds for Augmented Osteosarcoma Phototherapy and Accelerated Tissue Reconstruction

Iron oxides nanoparticles (IOs) exposed to magnetic field promote expression of osteogenic markers in osteoblasts through integrin alpha-3 (INTa-3) activation, inhibits osteoclasts activity and exerts anti-inflammatory action

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