Regulation of ornithine decarboxylase by antizymes and antizyme inhibitor in zebrafish (Danio rerio)

Hascilowicz, Tomasz; Murai, Noriyuki; Matsufuji, Senya; Murakami, Yasuko

doi:10.1016/s0167-4781(02)00476-1

Cited by 19 publications

(13 citation statements)

References 39 publications

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“…However, these data are inconsistent with the observed increase in protein biosynthesis-related genes. It has been previously reported that mammalian ODC protein has a very short half-life; its control is under negative feedback regulation by the polyamines, and its degradation is dependent on 26S proteasome complex (Hascilowicz et al 2002). Interestingly, we noted an increase in proteasome subunit (Psma7) mRNA expression levels.…”

Section: Involvement Of Protein Biosynthesis-related Genesmentioning

confidence: 47%

Renal Toxicogenomic Response to Chronic Uranyl Nitrate Insult in Mice

Taulan¹,

Paquet²,

Maubert³

et al. 2004

Environ Health Perspect

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Although the nephrotoxicity of uranium has been established through numerous animal studies, relatively little is known about the effects of long-term environmental uranium exposure. Using a combination of conventional biochemical studies and serial analysis of gene expression (SAGE), we examined the renal responses to uranyl nitrate (UN) chronic exposure. Renal uranium levels were significantly increased 4 months after ingestion of uranium in drinking water. Creatinine levels in serum were slightly but significantly increased compared with those in controls. Although no further significant differences in other parameters were noted, substantial molecular changes were observed in toxicogenomic profiles. UN induced dramatic alterations in expression levels of more than 200 genes, mainly up-regulated, including oxidative-response-related genes, genes encoding for cellular metabolism, ribosomal proteins, signal transduction, and solute transporters. Seven differentially expressed transcripts were confirmed by real-time quantitative polymerase chain reaction. In addition, significantly increased peroxide levels support the implication of oxidative stress in UN toxicant response. This report highlights the potential of SAGE for the discovery of novel toxicant-induced gene expression alterations. Here, we present, for the first time, a comprehensive view of renal molecular events after uranium long-term exposure.

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Section: Involvement Of Protein Biosynthesis-related Genesmentioning

confidence: 47%

Renal Toxicogenomic Response to Chronic Uranyl Nitrate Insult in Mice

Taulan¹,

Paquet²,

Maubert³

et al. 2004

Environ Health Perspect

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“…Recently, the AZI sequence for the model organism zebrafish (Danio rerio) became available [33]. All 27 residues important for mammalian ODC activity are present within the zebrafish ODC sequence.…”

Section: Formation Of the Active Site: A Comparison Between Odc And Azimentioning

confidence: 99%

“…Interestingly, it has been shown that AZI acts as a general inactivator of antizyme function. Zebrafish has two antizymes, which bind and inhibit ODC and are both inactivated by AZI [33]. Human AZI can bind and inactivate four members of the antizyme family, though human antizyme 1 and 2 share only 55% amino acid identity, and antizyme 3 is even more divergent (26 and 29% identity to antizyme 1 and 2, respectively) [36].…”

Section: Antizyme/azi Interaction and Its Consequencesmentioning

confidence: 99%

Antizyme inhibitor: mysterious modulator of cell proliferation

Mangold

2006

Cell. Mol. Life Sci.

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In contrast to the considerable interest in the oncogene ornithine decarboxylase (ODC) and in the family of antizymes with regard to cell proliferation and tumorigenesis, the endogenous antizyme inhibitor (AZI) has been less well studied. AZI is highly homologous to the enzyme ODC but does not possess any decarboxylase activity. Elevated ODC activity is associated with most forms of human malignancies. Antizymes bind ODC, inhibit ODC activity and promote the ubiquitin-independent degradation of ODC. Consequently they are proposed as tumor suppressors. In particular, the most studied member of the antizyme family, antizyme 1, has been demonstrated to play a role in tumor suppression. AZI inactivates all members of the antizyme family, reactivates ODC and prevents the proteolytic degradation of ODC, which may suggest a role for AZI in tumor progression.

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“…Previous research has described the identification, expression pattern and biochemical activity of two antizyme paralogs in the zebrafish Danio rerio (Saito et al, 2000;Hascilowicz et al, 2002). Both genes, called AZS and AZL (for AntiZyme Short and Long, though the AZL protein is only 4 amino acids longer), are expressed ubiquitously in embryos with the highest expression being in the central nervous system and in the lens (Saito et al, 2000).…”

Section: Introductionmentioning

confidence: 99%