Regulation of nodulin gene expression

Bruijn, Frans J. de; Chen, Rujin; Fujimoto, Susan Y.; Пинаев, А. Г.; Silver, David L.; Szczygłowski, Krzysztof

doi:10.1007/978-94-011-1088-4_6

Cited by 11 publications

(10 citation statements)

References 27 publications

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“…No additional signi®cant homologies to other promoters active in root nodules were found. As might be expected from its expression properties, complete organ-speci®c-elements (OSEs) or nodule-infected cell expression (NICE) elements (Metz et al 1988;de Bruijn et al 1994) are lacking in MtSucS1. Only the nodulin consensus sequences AAAGAT and CTCTT found in several promoters of genes expressed in root nodules (Stougaard et al 1987(Stougaard et al , 1990Ramlov et al 1993;Szczyglowski et al 1994;KuÈ ster et al 1995a) are present on both strands of the MtSucS1 promoter, six (AAA-GAT) and two times (CTCTT), respectively (Fig.…”

Section: Mtsucs1 Is a Single-copy Genementioning

confidence: 97%

Genomic organization and expression properties of the MtSucS1 gene, which encodes a nodule-enhanced sucrose synthase in the model legume Medicago truncatula

Hohnjec¹,

Becker²,

Pühler³

et al. 1999

Mol Gen Genet

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We have isolated and sequenced a sucrose synthase (SucS) cDNA from the model legume Medicago truncatula. This cDNA (MtSucS1) contains an ORF of 2418 bp, coding for a protein of 805 amino acids with a molecular mass of 92.29 kDa. The deduced amino acid sequence shows significant homology to other plant sucrose synthases, in particular to the nodule-enhanced sucrose synthases from pea and broad bean. Northern analysis revealed that the corresponding gene shows a ten-fold higher expression level in root nodules than in uninfected root, stem and leaf tissues. SucS protein was detected in root nodules from a variety of legumes, including M. truncatula. Whereas only one SucS isoform was detectable in root nodules, an additional sucrose synthase of slightly larger molecular weight was present in uninfected root, stem and flower tissues of M. truncatula. From our expression and sequence data we infer that the MtSucS1 gene encodes a nodule-enhanced sucrose synthase in M. truncatula. Southern hybridization data indicate that MtSucS1 is a single-copy gene. An analysis of a genomic MtSucS1 sequence revealed that the gene consists of 14 exons with the start codon being located on exon II. As is common for SucS genes, the MtSucS1 gene contains a large intron of 747 bp in the 5' untranslated region. The transcriptional start of MtSucS1 was mapped and putative regulatory elements in the MtSucS1 promoter were identified.

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Section: Mtsucs1 Is a Single-copy Genementioning

confidence: 97%

Genomic organization and expression properties of the MtSucS1 gene, which encodes a nodule-enhanced sucrose synthase in the model legume Medicago truncatula

Hohnjec¹,

Becker²,

Pühler³

et al. 1999

Mol Gen Genet

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“…On the other hand, we cannot completely exclude the possibility that the detection of two primer extension products constitutes a reverse transcriptase artifact. A number of sequence elements involved in mediating nodule-specific expression have already been identified for several late nodulin gene promoters [9,10]. Computer searches for these motifs identified five and three copies of the common nodulin sequences AAAGAT and CTCTT, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…These analyses resulted in the identification of cis-acting elements conserved amongst different late nodulin gene promoters [ 9,10]. The most extensively studied promoter elements are the nodule-infected cell expression element (NICE) and the organ-specific element (OSE) of the Sesbania rostrata and the soybean leghemoglobin gene promoters, respectively [ 10,41 ]. Both elements contained the common nodulin sequences 'AAAGAT' and 'CTCTT' [46,52].…”

Section: Introductionmentioning

confidence: 99%

The nodule-specific VfENOD-GRP3 gene encoding a glycine-rich early nodulin is located on chromosome I of Vicia faba L. and is predominantly expressed in the interzone II-III of root nodules

et al. 1995

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A nodule-specific cDNA was isolated from a Vicia faba L. nodule cDNA library. Since time course experiments revealed an early expression of this transcript in the nodule, this cDNA coded for an early nodulin and was designated VfENOD-GRP3. Based on tissue print hybridizations, we found a predominant expression of VfENOD-GRP3 transcripts in the interzone II-III region of broad bean root nodules. The encoded early nodulin ENOD-GRP3 was characterized by an N-terminal signal peptide and a C-terminal domain displaying a glycine content of 31%. Sequence analysis of a genomic VfENOD-GRP3 clone revealed that the signal peptide and the glycine-rich domain were specified by two separate exons. Primer extension experiments identified two adjacent transcription start sites for VfENOD-GRP3 transcripts. The common nodulin sequences 'AAAGAT' and 'CTCTT' were present five and three times on both DNA strands of the putative VfENOD-GRP3 promoter, respectively. Additionally, three sequence motifs resembling organ-specific elements of the soybean lbc3 gene promoter and a sequence similar to the binding site 1 for the nodule trans-acting factor Nat2 were identified. From Southern blot data and from sequence analysis of genomic PCR fragments, the presence of a VfENOD-GRP3 gene family was inferred. By PCR experiments using sequence-specific primers and DNA of microisolated chromosomes as a template, this family was located on the long arm of chromosome I.

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“…From these analyses it is evident that the legume nodulin genes have evolved from non-legume genes that are expressed under the control of dierent stimuli and under dierent circumstances. Experiments designed to reveal the molecular basis of the expression of nodulin genes in root nodules have led to the identi®cation of regulatory elements, such as the organ speci®c elements (OSE) and the nodule infected cell expression (NICE) elements (Metz et al 1988;de Bruijn et al 1994). These include the nodulin consensus sequences AAA-GAT and CTCTT, which are present in the promoters of several genes expressed in root nodules (Stougaard et al 1987(Stougaard et al , 1990Ramlov et al 1993;Szczyglowski et al 1994).…”

Section: Introductionmentioning

confidence: 99%

The broad bean nodulin VfENOD18 is a member of a novel family of plant proteins with homologies to the bacterial MJ0577 superfamily

et al. 2000

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Full-length transcript sequences were isolated from broad bean root nodules, which encode a novel nodulin designated VfENOD18. The corresponding transcripts were detected in early and in late stages of nodule development and were localized exclusively in the nitrogen-fixing zone III. The VfENOD18 sequence is not only homologous to a number of ESTs from various mono- and dicotyledonous plants, but also to the ATP-binding protein MJ0577 from Methanococcus jannaschii and to a range of bacterial proteins that belong to the MJ0577 superfamily. Hence, VfENOD18 is a member of a ubiquitous family of plant proteins that might function as ATP-binding proteins or ATPases. On the genomic level, VfENOD18 genes can be divided into two groups on the basis of differences in their 5' UTRs. One group lacks the 5' UTR region including the ATG initiation codon, whereas the second group contained the complete 5' UTR region. Further upstream of this VfENOD18 gene, a retrotransposon sequence was identified. The -14/-964 VfENOD18 promoter fragment was devoid of complete organ-specific elements known from other nodulin gene promoters. Nevertheless, this region was able to mediate full promoter activity in the central region of transgenic Vicia hirsuta root nodules.

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Regulation of nodulin gene expression

Cited by 11 publications

References 27 publications

Genomic organization and expression properties of the MtSucS1 gene, which encodes a nodule-enhanced sucrose synthase in the model legume Medicago truncatula

Genomic organization and expression properties of the MtSucS1 gene, which encodes a nodule-enhanced sucrose synthase in the model legume Medicago truncatula

The nodule-specific VfENOD-GRP3 gene encoding a glycine-rich early nodulin is located on chromosome I of Vicia faba L. and is predominantly expressed in the interzone II-III of root nodules

The broad bean nodulin VfENOD18 is a member of a novel family of plant proteins with homologies to the bacterial MJ0577 superfamily

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