Regulation of nitrogen metabolism and gene expression in fungi.

Marzluf, George A.

doi:10.1128/mmbr.45.3.437-461.1981

Cited by 202 publications

(80 citation statements)

References 49 publications

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“…that when primary sources of nitrogen, e.g. ammonium or glutamine, are limiting, fungal cells derepress the expression of sets of genes that allow the utilization of secondary nitrogen sources such as nitrate, nitrite, purines, amino acids and proteins (Marzluf, 1981). However, as presented in Figure 2, the addition of 10 mM ammonium, 10 mM glutamine or 10 mM nitrate had no effect on ME-related lac-J transcript accumulation.…”

Section: Resultsmentioning

confidence: 95%

Altered transcriptional response to nutrient availability in hypovirus-infected chestnut blight fungus.

Larson

Nuss

1994

The EMBO Journal

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The gene lac‐1, encoding the enzyme laccase, is one of several genes of the chestnut blight fungus, Cryphonectria parasitica, that are suppressed by virulence‐attenuating mycoviruses of the hypovirus group. Two antagonistic regulatory pathways have been shown to govern the activity of the lac‐1 promoter: a positive pathway that stimulates transcription and a negative pathway that represses transcription. We now report that these two regulatory pathways respond independently to specific changes in the nutritional environment. These newly defined conditions were used to confirm that a hypovirus suppresses the activity of the positive regulatory pathway, and to implicate calmodulin and calcineurin as components of the signal transduction cascades regulating lac‐1 transcription. Significantly, lac‐1 transcript accumulation was shown to be affected by amino acid availability. Further analysis revealed that transcriptional repression mediated by the negative regulatory pathway is relieved under conditions of amino acid deprivation. Thus, by blocking the positive pathway, hypovirus infection prevents increased lac‐1 transcript accumulation in response to amino acid deficiency. These observations are consistent with the hypothesis that hypoviruses alter the transcriptional response of the host fungus to changes in nutrient availability.

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Section: Resultsmentioning

confidence: 95%

Altered transcriptional response to nutrient availability in hypovirus-infected chestnut blight fungus.

Larson

Nuss

1994

The EMBO Journal

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“…Glutamine plays, together with glutamic acid, a crucial role in assimilating ammonia and in acting as a nitrogen donor for the synthesis of other metabolites. Additionally, glutamine is possibly involved in the regulation of other nitrogen-related enzymes [8].…”

Section: Discussionmentioning

confidence: 99%

Double‐quantum ¹H NMR studies of nitrogen metabolism in yeast

Juretschke

1984

FEBS Letters

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Using double‐quantum 1H NMR spectroscopy, the nitrogen metabolism of yeast cells pulse‐labelled with 15N‐enriched ammonium sulfate has been detected. The sensitivity of this indirect method is far higher than direct observation of the 15N signals by 15N NMR. Hence, a spectrum with an excellent signal‐to‐noise ratio was obtained in 25.6 s. The observed proton signals were assigned to specific metabolites using two‐dimensional reverse shift correlation spectra.

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“…Therefore, it is reasonable to suggest that NADP-GDH degradation could be modulated by two different systems that can act in combination on the same protein. Inactivation of nitrate reductase in N. crassa proceeds through such a mechanism [18].…”

Section: Involved In Carbon-starvation-induced Nadp-gdh Degradation mentioning

confidence: 99%

The role of glutamate in modulating the synthesis and degradation of NADP-glutamate dehydrogenase from Saccharomyces cerevisiae

Bogónez

Satrústegui

Machado

1985

FEMS Microbiology Letters

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NADP‐glutamate dehydrogenase (NADP‐GDH) from Saccharomyces cerevisiae has a lower activity in yeast grown on glutamate as nitrogen source than when grown on ammonium. With the use of the immunotitration method, it was found that the difference in activity was parallel to the difference in immunoprecipitable material. By isotope incorporation studies, it was established that the decrease in NADP‐glutamate dehydrogenase levels in glutamate‐grown cells was brought about by an increase in the degradation rate and a decrease in the synthesis constant of the enzyme. The degradation rate of NADP‐glutamate dehydrogenase is further increased in carbon‐starved cells. The possible role of internal metabolites in modulating NADP‐glutamate dehydrogenase degradation is discussed.

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Regulation of nitrogen metabolism and gene expression in fungi.

Cited by 202 publications

References 49 publications

Altered transcriptional response to nutrient availability in hypovirus-infected chestnut blight fungus.

Altered transcriptional response to nutrient availability in hypovirus-infected chestnut blight fungus.

Double‐quantum ¹H NMR studies of nitrogen metabolism in yeast

The role of glutamate in modulating the synthesis and degradation of NADP-glutamate dehydrogenase from Saccharomyces cerevisiae

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Regulation of nitrogen metabolism and gene expression in fungi.

Cited by 202 publications

References 49 publications

Altered transcriptional response to nutrient availability in hypovirus-infected chestnut blight fungus.

Altered transcriptional response to nutrient availability in hypovirus-infected chestnut blight fungus.

Double‐quantum 1H NMR studies of nitrogen metabolism in yeast

The role of glutamate in modulating the synthesis and degradation of NADP-glutamate dehydrogenase from Saccharomyces cerevisiae

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Product

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Double‐quantum ¹H NMR studies of nitrogen metabolism in yeast