Regulation of nitrogen fixation in Rhodospirillum rubrum grown under dark, fermentative conditions

Schultz, J. E.; Gotto, John W.; Weaver, Paul F.; Yoch, Duane C.

doi:10.1128/jb.162.3.1322-1324.1985

Cited by 18 publications

(8 citation statements)

References 18 publications

(17 reference statements)

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“…Dark anaerobic nitrogen-fixing cultures were grown as described by Schultz et al (1985) in miminal medium containing fructose (11 mM) as the carbon source, and sodium bicarbonate (17 mM). Dark anaerobic fermenting cultures were grown with pyruvate as the sole carbon source and ammonium as the sole nitrogen source.…”

Section: Methodsmentioning

confidence: 99%

**Identification and sequence of a nifJ‐like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation**

Lindblad

Jansson

Brostedt

et al. 1996

Molecular Microbiology

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A nifJ-like gene was identified in the photosynthetic purple non-sulphur bacterium Rhodospirillum rubrum. A DNA segment hybridizing to Klebsiella pneumoniae nifJ was isolated, the gene was inactivated, and a mutant strain, SNJ-1, was constructed by allele replacement. The mutation was confirmed by DNA sequencing. Northern blotting and by the lack of pyruvate oxidoreductase activity. This is the first report of a nifJ-like gene in photosynthetic bacteria. Unexpectedly, SNJ-1 was capable of nitrogen fixation, and growth was similar to the wild-type strain under all conditions investigated. Therefore, this is also the first demonstration that a nifJ homologue, when present, is not essential for nitrogen fixation in a diazotroph. The nifJ-like gene was sequenced and found to have considerable similarity to published nifJ gene sequences from other organisms. By primer extension, the initiation site for transcription was located, and a typical sigma 54 promoter sequence was identified.

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Section: Methodsmentioning

confidence: 99%

**Identification and sequence of a nifJ‐like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation**

Lindblad

Jansson

Brostedt

et al. 1996

Molecular Microbiology

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“…Extracts from cells, which were grown under fixed-nitrogen-limited conditions, show high nitrogenase activity and very little of the modified form, indicating that N-starved cells also are insensitive to darkness. The inhibition induced by addition of NH 4 + was also demonstrated in R. rubrum grown under dark, fermentative conditions (Schultz et al, 1985).…”

Section: Composition Of the Modifying Group And Its Locationmentioning

confidence: 81%

“…The region upstream of draT does not contain any typical nif promoter, suggesting that transcription of the nif and the dra genes are not co-regulated. This suggestion is supported by DRAG being detected under all growth conditions studied (Triplett et al, 1982) and by the demonstration that R. rubrum, when grown fermentatively in the dark, responds to added NH 4 + and so has an active DRAG (Schultz et al, 1985). As in R. rubrum, the dra genes in Az.…”

Section: Genetics Of the Drat/drag Systemmentioning

confidence: 83%

Post-Translational Regulation of Nitrogenase in Photosynthetic Bacteria

Nordlund

Ludden

Genetics and Regulation of Nitrogen Fixation in Free-Living Bacteria

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The photosynthetic bacteria are remarkable for their range of metabolic capabilities and, as a result, the ability to survive under a wide range of environmental conditions, including nitrogen limitation. The photosynthetic bacteria are members of the alpha subdivision of the proteobacteria (Imhoff et al., 1984;Woese et al., 1984) and, with a single known exception, the wild-type strains of all purple sulfur bacteria and all purple non-sulfur bacteria are capable of N 2 fixation (Madigan et al., 1984). Most of the work that will be described in this chapter has been performed on a few species of purple, non-sulfur bacteria, including Rhodospirillum rubrum and Rhodobacter capsulatus. This chapter will focus on the biochemistry and physiology of N 2 fixation by photosynthetic bacteria and the regulation of nitrogenase activity by reversible ADP-ribosylation of the dinitrogenase reductase (Fe protein). ADP-ribosylation has also been found in Azospirillum and other genera and, where relevant, that work will be included. Other chapters in this series will cover the genes of the nif, anf and rnf regulons of the photosynthetic bacteria and the regulation of the expression of those genes. Although this chapter will discuss aspects of the nitrogenase enzyme, the mechanistic and structural details of nitrogenase will be covered in more detail in Volume 1 of this series.

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“…Nearly all isolates of photosynthetic bacteria have a nitrogenase enzyme complex (Weaver et al 1975), which, in the absence of ammonium ion or dinitrogen gas and in the presence of oxidizable organic materials, functionsto reduce protonsand evolve hydrogen. Hydrogen evolution is largely light dependent (Schultz et al 1985) and strongly exergonic. The hydrolysis of about 4 ATP (largely synthesized in light) is requiredto generate each H2and can drive the gas productionto equilibriumpressuresin excess of I00 atmospheres.…”

Section: Resultsmentioning

confidence: 99%

Proceedings of the 1994 DOE/NREL Hydrogen Program Review, 18-21 April 1994, Livermore, California

Author¹

1994

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This reportwas prepared as an accountof work sponsoredby an agency of the United States government.Neither the United States governmentnorany agency thereof, nor any of theft"employees, makes any warranty, expressor implied, or assumesany legal liabilityor responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or processdisclosed,or representsthatits usewouldnotinfringeprivatelyownedrights. Reference herein to any specificcommercialproduct,process,or service by trade name, trademark,manufacturer,or otherwisedoes not necessarily constituteor imply itsendorsement,recommendation, or favoringby the UnitedStatesgovernmentor any agencythereof.The viewsand opinionsof authors expressedherein do notnecessarilystateor reflectthoseof the UnitedStates governmentor any agencythereof.

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Regulation of nitrogen fixation in Rhodospirillum rubrum grown under dark, fermentative conditions

Cited by 18 publications

References 18 publications

**Identification and sequence of a nifJ‐like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation**

**Identification and sequence of a nifJ‐like gene in Rhodospirillum rubrum: partial characterization of a mutant unaffected in nitrogen fixation**

Post-Translational Regulation of Nitrogenase in Photosynthetic Bacteria

Proceedings of the 1994 DOE/NREL Hydrogen Program Review, 18-21 April 1994, Livermore, California

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