Regulation of NF-κB signaling by oxidized glycerophospholipid and IL-1β induced miRs-21-3p and -27a-5p in human aortic endothelial cells

MicroRNA‐351‐5p aggravates intestinal ischaemia/reperfusion injury through the targeting of MAPK13 and Sirtuin‐6

“…Among them, 18 up‐regulated miRNAs (miR‐23a‐5p, miR‐300‐5p, miR‐21‐3p, miR‐344b‐1‐3p, miR‐351‐5p, miR‐874‐3p, miR‐144‐3p, miR‐3572, miR‐27a‐5p, miR‐183‐3p, miR‐294, miR‐451‐5p, miR‐483‐3p, miR‐883‐5p, miR‐125b‐2‐3p, miR‐3573‐3p, miR‐485‐3p and miR‐490‐5p) and 12 down‐regulated miRNAs (miR‐181c‐5p, miR‐187‐3p, miR‐532‐5p, miR‐194‐5p, miR‐203a‐3p, miR‐17‐1‐3p, miR‐192‐5p, miR‐28‐3p, miR‐140‐5p, miR‐142‐3p, miR‐29c‐3p and miR‐362‐3p) with clear statistical significance ( P <0.05 different from control values) were identified. Furthermore, nine miRNAs ‐ miR‐144‐3p, miR‐3572, miR‐27a‐5p, miR‐142‐3p, miR‐23a‐5p, miR‐21‐3p, miR‐294, miR‐351‐5p and miR‐362‐3p ‐ associated with oxidative stress, inflammation and apoptosis (Romay et al ., ; Wang et al ., ; da Silva et al ., ; Dixit et al ., ; Brea et al ., ; Chen et al ., ; Li et al ., ) were selected for validation by real‐time PCR assay in IEC‐6 cells and rats. These results, shown in Figure B, were consistent with the results of the microarray assays.…”

“…All data are expressed as the mean ± SD. * P < 0.05, significantly different from control group; # P < 0.05, significantly different from the 60 min reoxygenation or 90 min reperfusion group.miRNA-351-5p aggravates II/R injury 351-5p and miR-362-3p -associated with oxidative stress, inflammation and apoptosis(Romay et al, 2015;Wang et al, 2015;da Silva et al, 2016;Dixit et al, 2016;Brea et al, 2017;Chen et al, 2017;Li et al, 2018) were selected for validation by real-time PCR assay in IEC-6 cells and rats. These results, shown inFigure 3B, were consistent with the results of the microarray assays.…”

Genomic Analysis of miR-21-3p and Expression Pattern with Target Gene in Olive Flounder

“…In the previous study, GO enrichment in the genes noted serious enhancement for target mRNAs. These targets were related to the defense response, inflammatory response, and immunological reactions, which the result revealed nuclear factor-kB signaling pathway by using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway program [28]. Also, the possible regulatory pathways for the miR-21-3p at major target genes were analyzed by KEGG terms.…”

miR-21, a potential therapeutic target of alleviating blood-brain barrier damage after traumatic brain injury

“…Besides, miR-21-3p can activate the Akt signaling that regulates cellular apoptosis in breast tumors [23] . The research on human aortic endothelial cells also found that miR-21-3p is an up-stream regulator of NF-kB signaling that can regulate the immuno-inflammatory responses [24] . In the research on cardiac hypertrophy, miR-21-3p has been proved to have the function of impacting the hypertrophic progress of cradiomyocytes induced by Ang-2 infusion, suggesting that miR-21-3p could regulate the expression of angiogenic factors, such as Ang-1 and Ang-2 [25][26] .…”