Regulation of neurotensin content in adrenal medullary cells: Comparison of PC12 cells to normal rat chromaffin cells in vitro

Tischler, Arthur S.; Ruzicka, Laurel A.; DeLellis, Ronald A.

doi:10.1016/0306-4522(91)90325-i

Cited by 9 publications

(4 citation statements)

References 32 publications

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“…The studies by Caillaud et al 37 and Tischler et al 38 demonstrated that heterogeneity of Nts expression in PC12 cells reflects different states of differentiation. Treatment with dexamethasone and nerve growth factor induced differentiation and expression of Nts.…”

Section: Discussionmentioning

confidence: 99%

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Opposing effects of HIF1α and HIF2α on chromaffin cell phenotypic features and tumor cell proliferation: Insights from MYC‐associated factor X

Qin

Cubas

García‐Martín

et al. 2014

Intl Journal of Cancer

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Pheochromocytomas and paragangliomas (PPGLs) are catecholamine-producing chromaffin cell tumors with diverse phenotypic features reflecting mutations in numerous genes, including MYC-associated factor X (MAX). To explore whether phenotypic differences among PPGLs reflect a MAX-mediated mechanism and opposing influences of hypoxia-inducible factor (HIF)s HIF2a and HIF1a, we combined observational investigations in PPGLs and gene-manipulation studies in two pheochromocytoma cell lines. Among PPGLs from 140 patients, tumors due to MAX mutations were characterized by gene expression profiles and intermediate phenotypic features that distinguished these tumors from other PPGLs, all of which fell into two expression clusters: one cluster with low expression of HIF2a and mature phenotypic features and the other with high expression of HIF2a and immature phenotypic features due to mutations stabilizing HIFs. Max-mutated tumors distributed to a distinct subcluster of the former group. In cell lines lacking Max, re-expression of the gene resulted in maturation of phenotypic features and decreased cell cycle progression. In cell lines lacking Hif2a, overexpression of the gene led to immature phenotypic features, failure of dexamethasone to induce differentiation and increased proliferation. HIF1a had opposing actions to HIF2a in both cell lines, supporting evolving evidence of their differential actions on tumorigenic processes via a MYC/MAX-related pathway. Requirement of a fully functional MYC/MAX complex to facilitate differentiation explains the intermediate phenotypic features in tumors due to MAX mutations. Overexpression of HIF2a in chromaffin cell tumors due to mutations affecting HIF stabilization explains their proliferative features and why the tumors fail to differentiate even when exposed locally to adrenal steroids.

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Section: Discussionmentioning

confidence: 99%

“…The studies by Caillaud et al . and Tischler et al . demonstrated that heterogeneity of Nts expression in PC12 cells reflects different states of differentiation.…”

Section: Discussionmentioning

confidence: 99%

Opposing effects of HIF1α and HIF2α on chromaffin cell phenotypic features and tumor cell proliferation: Insights from MYC‐associated factor X

Qin

Cubas

García‐Martín

et al. 2014

Intl Journal of Cancer

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“…Binding of NT to NTR1 stimulates phosphorylation of ERK and AKT (Hassan et al, 2004) and probably contributes to the activation of ERK and AKT signalling pathways in the carotid body and peripheral ganglia (Porzionato et al, In press). Cell line PC12, which shows oxygen-sensing properties and has been considered as a model for carotid body function, cannot be used for investigation of NT action, as these cells express and release neurotensin (Tischler et al, 1991) but seem not to show functional NTR (Caillaud et al, 1995). However, it is intriguing to note that NT attenuates dopamine-induced inhibition of midbrain dopamine neurons (Seutin et al, 1989;Shi et al, 1992), as dopamine is the main glomic neurotransmitter.…”

Section: Discussionmentioning

confidence: 99%

Neurotensin receptor 1 immunoreactivity in the peripheral ganglia and carotid body

Porzionato¹,

Macchi²,

Amagliani³

et al. 2009

Eur J Histochem

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In the present study we investigated, through immunohistochemistry, the presence and location of neurotensin receptor 1 (NTR1) in the peripheral ganglia and carotid body of 16 humans and 5 rats. In both humans and rats, NTR1 immunostained ganglion cells were found in superior cervical ganglia (57.4±11.6% and 72.4±11.4%, respectively, p<0.05), enteric ganglia (51.9±10.4% and 64.6±6.1%, p<0.05), sensory ganglia (69.2±10.7% and 73.0±13.1%, p>0.05) and parasympathetic ganglia (52.1±14.1% and 59.4±14.0%, p>0.05), supporting a modulatory role for NT in these ganglia. Positivity was also detected in 45.6±9.2% and 50.8±6.8% of human and rat type I glomic cells, respectively, whereas type II cells were negative. Our findings suggest that NT produced by type I cells acts in an autocrine or paracrine way on the same cell type, playing a modulatory role on chemoception.

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“…Binding of NT to NTR1 stimulates phosphorylation of ERK and AKT (Hassan et al , 2004) and probably contributes to the activation of ERK and AKT signalling pathways in the carotid body and peripheral ganglia (Porzionato et al , In press). Cell line PC12, which shows oxygen-sensing properties and has been considered as a model for carotid body function, cannot be used for investigation of NT action, as these cells express and release neurotensin (Tischler et al , 1991) but seem not to show functional NTR (Caillaud et al , 1995). However, it is intriguing to note that NT attenuates dopamine-induced inhibition of midbrain dopamine neurons (Seutin et al , 1989; Shi et al , 1992), as dopamine is the main glomic neurotransmitter.…”

Section: Discussionmentioning

confidence: 99%

Neurotensin receptor 1 immunoreactivity in the peripheral ganglia and carotid body

Porzionato¹,

Macchi²,

Amagliani³

et al. 2009

Eur J Histochem

View full text Add to dashboard Cite

show abstract

Regulation of neurotensin content in adrenal medullary cells: Comparison of PC12 cells to normal rat chromaffin cells in vitro

Cited by 9 publications

References 32 publications

Opposing effects of HIF1α and HIF2α on chromaffin cell phenotypic features and tumor cell proliferation: Insights from MYC‐associated factor X

Opposing effects of HIF1α and HIF2α on chromaffin cell phenotypic features and tumor cell proliferation: Insights from MYC‐associated factor X

Neurotensin receptor 1 immunoreactivity in the peripheral ganglia and carotid body

Neurotensin receptor 1 immunoreactivity in the peripheral ganglia and carotid body

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