The entire nervous system of the vertebrate embryo is derived from an epithelium sheet called the neural plate, which is induced by the organizer/node at the onset of gastrulation. Dissection of the molecular network underlying the emergence of the neural plate, originally performed in
Xenopus
embryos, led to the discovery of the TGFβ double inhibition mechanism for neural induction, coined ‘the default model’. Years later, these developmental principles have been translated
in vitro
to instruct human pluripotent stem cells towards neural fate and to build brain organoids.
Key Concepts
The Spemann organizer is defined by the capability of a group of cells in a transplantation experiment to induce, in a non‐cell‐autonomous fashion, a secondary neural axis and to autonomously differentiate into axial and paraxial mesoderm derivatives.
Neural fate acquisition both
in vivo
in the embryo and
in vitro
from pluripotent stem cells is the result of blocking the ongoing TGFβ pathway, both Activin‐Nodal and BMP branches.
Embryonic stem cells are defined based on two basic properties: their ability to maintain their undifferentiated state (stemness) indefinitely and to be able to differentiate into cell types derived from the three embryonic germ layers (pluripotency).
Brain organoids are 3D
in vitro
structures that resemble several features of the developing human brain.
Human pluripotent stem cells cultured in confined geometry (micropattern) self‐organized into defined embryonic structures with standardised cytoarchitecture and distinct cell fates.