Regulation of Na+,K+-ATPases II. Cloning and analysis of the 5′-flanking region of the rat NKAB2 gene encoding the ß2 subunit

Kawakami, Kiyoshi; Okamoto, Hiroshi; Yagawa, Yuriko; Nagano, Kei

doi:10.1016/0378-1119(90)90099-d

Cited by 19 publications

(11 citation statements)

References 8 publications

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“…Thus, RMER3 repeats may be solo LTRs from an unknown rat retrovirus. Two glucocorticoid responsive elements (positions 341-346 and 429-434) and an ATF bound moiety (580-586) have been identified (Kawakami et al, 1990) in the RMER3 sequence from the 5' flanking region of the NKPB2 gene (GenBank accession no. D90048).…”

Section: Rmer3 Familymentioning

confidence: 99%

Identification of new medium reiteration frequency repeats in the genomes of Primates, Rodentia and Lagomorpha

et al. 1996

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We report eleven new families of MEdium Reiteration frequency (MER) interspersed repeats in the genomes of Primates, Rodentia, and Lagomorpha. Two families of the human repeats, MER 46 and MER 47, represent non-autonomous DNA transposons. These sequences are flanked by TA target site duplications and have terminal inverted repeats (TIRs) similar to TIRs of DNA transposons. The sequences of five other families of repeats, MER41, MER48, MER50, MER51, and RMER3, resemble long terminal repeats of retroviruses. A potential involvement of some of the reported MER repeats in the regulation of transcription and genetic rearrangements is suggested. Age estimations place the origin of most MER repeats at the time of decline in MIR (Mammalian-wide Interspersed Repeats) retroposition and before the origin of the Alu family.

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Section: Rmer3 Familymentioning

confidence: 99%

Identification of new medium reiteration frequency repeats in the genomes of Primates, Rodentia and Lagomorpha

et al. 1996

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“…To reveal the molecular mechanisms of the tissue-specific, developmental, and hormonal regulation of Na,K-ATPase gene expression and of the coordinated regulation between the a-and 13-subunit genes, we have performed a systematic analysis of the genes encoding a-and 13-subunit isoforms, including their 5'-flanking regions where important regulatory functions reside (15,17,18,43). We cloned from the rat chromosome a 13.3-kb DNA fragment that included the Na,K-ATPase al subunit gene (ATPlA1) and 5' flanking region (EMBL/GenBank data library accession number X51461), and identified the transcription initiation site at 262 bp upstream from the translation initiation codon (43).…”

mentioning

confidence: 99%

Housekeeping Na,K-ATPase alpha 1 subunit gene promoter is composed of multiple cis elements to which common and cell type-specific factors bind.

Suzuki-Yagawa¹,

Kawakami²,

Nagano³

1992

Mol. Cell. Biol.

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Na,K-ATPase al subunit gene (ATPlA1) is one of the housekeeping genes involved in homeostasis of Na+ and K+ in all animal cells. We identified and characterized the cis-acting elements that regulate the expression of ATPAL1. The region between -155 and -49 was determined as a positive regulatory region in five cultured cell lines of different tissue origins (MDCK, B103, L6, 3Y1, and HepG2). The region was divided into three subregions: from -120 to -106 (including the Spl binding site), from -102 to -61, and from -58 to -49 (including an Spl consensus sequence). Cell type-specific factors binding to the middle subregion (from -102 to -61) were detected by gel retardation analysis, using nuclear extracts prepared from MDCK and B103 cells. Two gel retardation complexes were formed in the B103 nuclear extract, and three were formed in the MDCK nuclear extract. DNA binding regions of these factors were located at -88 to -69 and differed from each other in DNase I footprinting experiments. These factors also showed different binding characteristics in gel retardation competition and methylation interference experiments. The identified cis element was named the ATPL1A regulatory element. The core sequence of this element is found in several other genes involved in cellular energy metabolism, suggesting that the sequence is a common regulatory element responsive to the state of energy metabolism.

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“…To determine whether the AMOG gene was expressed in cultured astrocytes used in this study, we performed an RNase protection assay. A uniformly labelled RNA probe that covers -76 to +570 of the rat AMOG gene [Kawakami et al, 1990;973 nucleotides (nts) including the region which covers the CAT gene and the polylinker region of pKSM13 + ] was prepared and used.…”

Section: Expression Of the Amog Gene In Primary Cultured Astrocytesmentioning

confidence: 99%

“…In the case of B103 cells, an 8.6-fold decrease was observed between pB2U-118CAT and pB2U-76CAT, while the CAT activity remained unchanged between the deletion mutants of pB2U-76CAT to pB2U-38CAT . Because the Spl binding site exists between -76 and -38 (Kawakami et al, 1990), we focused further analysis on the region between -118 and -76. For the purpose of identifying the precise location of the cis-element involved in the decrease of the promoter activity between pB2U-118CAT and pB2U-76CAT, the linker substitution mutants of pB2U-118CATLS2 (BglII linker substituted at positions -101 to -92) and pB2U-1 18CATLS3 (BgEII linker substituted at positions -87 to -78) were analyzed.…”

Section: Identification Of the Upstream Sequences That Regulate The Ementioning

confidence: 99%

Sp1 binds to the adhesion molecule on glia regulatory element that functions as a positive transcription regulatory element in astrocytes

Kawakami

Watanabe

Araki

et al. 1993

J of Neuroscience Research

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We analyzed cis-acting elements regulating the expression of the gene encoding adhesion molecule on glia (AMOG) in primary cultured astrocytes from newborn rat cerebrum and cerebellum. The relative promoter activities among the series of 5' sequential deletion mutants are similar to those observed in B103 (rat neuroblastoma cell line) cells. The previously identified AMRE (AMOG regulatory element) of the GAGGCGGGG sequence functions as a positive regulatory element, not only in B103 cells, but also in astrocytes. Binding factors to the element were identified as Sp1 based on the following observations using nuclear extracts from the astrocytes and B103 cells: (1) The interaction of the factors with AMRE analyzed by DNase I footprinting and methylation interference analyses was similar to that of Sp1; (2) The binding of the factors to AMRE competed with an oligonucleotide containing the authentic Sp1 consensus sequence; (3) Sp1-specific antibody interfered with the formation of the AMRE gel retardation complexes. The functional implications of the factors in AMOG gene regulation are discussed.

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Regulation of Na+,K+-ATPases II. Cloning and analysis of the 5′-flanking region of the rat NKAB2 gene encoding the ß2 subunit

Cited by 19 publications

References 8 publications

Identification of new medium reiteration frequency repeats in the genomes of Primates, Rodentia and Lagomorpha

Identification of new medium reiteration frequency repeats in the genomes of Primates, Rodentia and Lagomorpha

Housekeeping Na,K-ATPase alpha 1 subunit gene promoter is composed of multiple cis elements to which common and cell type-specific factors bind.

Sp1 binds to the adhesion molecule on glia regulatory element that functions as a positive transcription regulatory element in astrocytes

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