Regulation of Na+ absorption and Cl- secretion in the endometrium: switching mechanisms

Chan, Hsiao Chang; Chan, Li-Chong; Wang, Xiao Fei; Tsang, L.L.; So, S.C.

doi:10.3346/jkms.2000.15.s.s34

Cited by 4 publications

(2 citation statements)

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“…0:05). The forskolin-induced I sc , previously shown to be mediated by CFTR (Chan et al, 1997c(Chan et al, , 1999(Chan et al, , 2000a was also increased but to a lesser extent ( from 3.08 G 1.16 mA/cm 2 to 5.97 G 0.74 mA/cm 2 ; P ! 0:05) in aldosterone-treated endometrial epithelia.…”

Section: Effect Of Aldosterone On Enac and Cftr-mediated I Scmentioning

confidence: 76%

Altered cyclic expression of epithelial Na⁺ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet

Tsang

Chan

2004

Cell Biology International

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Epithelial Na+ channel (ENaC) and cystic fibrosis transmembrane conductance (CFTR) have been shown to exhibit cyclic expression patterns in the uterus and demonstrated to play important roles in regulating uterine fluid absorption and secretion. The present study investigated the effect of a low Na+ diet on the cyclic expression of uterine ENaC subunits and CFTR in mice. Ten to 12 weeks old ICR mice with synchronized estrus cycle were fed with a low sodium diet for at least 2 weeks and the mRNA level of these ion channels was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Functional channel activities in primary cultures of endometrial epithelia were assessed by the short-circuit current (Isc) technique. The characteristic cyclic expression of ENaC subunits throughout the estrus cycle remained unchanged but their expression levels towards the diestrus stage were drastically elevated. The cyclic expression pattern of CFTR was disrupted with suppressed expression throughout the cycle. Isc measurements showed that treatment of cultured endometrial epithelial cells with aldosterone, the major hormone expected to be elevated during the low sodium diet, resulted in prominent increase in ENaC channel activity. The altered cyclic expression of uterine ENaC and CFTR by a low sodium diet suggests that these ion channels may be affected by elevated circulating aldosterone, which may disrupt reproductive events in the uterus.

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Section: Effect Of Aldosterone On Enac and Cftr-mediated I Scmentioning

confidence: 76%

Altered cyclic expression of epithelial Na⁺ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet

Tsang

Chan

2004

Cell Biology International

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“…Data from our fertility study, the first of its kind, revealed upregulated PLIN2 in LF animals compared with HF animals. Numerous studies report electrolyte transport activities across the endometrial epithelium (12)(13)(14), thereby influencing uterine fluid composition and in turn intrauterine sperm capacitance and motility and embryo development and implantation. It has been observed that a high molar ratio of K ϩ /Na ϩ contributes to an improvement in in vitro fertility in rats (103).…”

Section: Continuedmentioning

confidence: 99%

Endometrial gene expression in high- and low-fertility heifers in the late luteal phase of the estrous cycle and a comparison with midluteal gene expression

Killeen

Diskin

Morris

et al. 2016

Physiological Genomics

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Embryonic mortality is a major constraint to improving reproductive efficiency and profitability in livestock enterprises. We previously reported differential expression of genes with identified roles in cellular growth and proliferation, lipid metabolism, endometrial remodeling, inflammation, angiogenesis, and metabolic exchange in endometrial tissue on day 7 of the estrous cycle (D7), between heifers ranked as either high (HF) or low (LF) for fertility. The aim of the current study was to further elucidate the underlying molecular mechanisms contributing to early embryo loss by examining differential endometrial gene expression in HF or LF heifers at a later stage of the estrous cycle;day 14(D14). A second objective was to compare these expression profiles with those from midluteal HF and LF endometrium. Using the same animal model as employed in the previous study, we slaughtered HF and LF animals on D14, harvested endometrial tissue, and carried out global gene expression analysis using the Affymetrix Bovine GeneChip. Microarray analysis detected 430 differentially expressed genes (DEG) between HF and LF animals. Ingenuity Pathway Analysis revealed enrichment for a host of biological pathways including lipid metabolism, molecular transport, immune response, cell morphology and development, and cell growth and proliferation. Important DEG includedALB, BMPR2, CCL28, COL4A3/4, FADS1, ITGA6, LDLR, PLCB3, PPARG, PTGS2, and SLC27A4 Furthermore, DEG expressed on both D7 and D14 included:PCCB,SLC25A24,DAP, and COL4A4 This study highlights some of the pathways and mechanisms underpinning late luteal bovine endometrial physiology and endometrial-related conception rate variance.

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Effect of Phenol Red and Steroid Hormones on Cystic Fibrosis Transmembrane Conductance Regulator in Mouse Endometrial Epithelial Cells

Tsang

Chan

Liu

et al. 2001

Cell Biology International

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Previous studies have demonstrated that cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-mediated Cl(-)channel found in most epithelia including reproductive tract, could be regulated by various culture conditions. The present study further investigated the effect of phenol red, a pH indicator widely used in growth medium, and steroid hormones, present in the supplement fetal bovine serum (FBS), on primary cultured endometrial epithelial cells by monitoring ion channel activities using the short-circuit current technique. When compared to the results obtained with normal medium supplemented with regular FBS, the forskolin-stimulated I(SC), presumably mediated by CFTR, obtained in phenol red-free medium was significantly reduced, from 16.95+/-1.53 microA/cm(2)(control) to 9.72+/-0.89 microA/cm(2)(medium without phenol red, P< 0.05). The forskolin-activated I(SC)was further attenuated to 5.29+/-0.46 microA/cm(2)in the phenol red-free medium when supplemented with charcoal/ dextran-treated FBS where steroid hormones were removed. Our data suggest that phenol red and steroid hormones present in culture medium and FBS supplement, respectively, may somehow upregulate CFTR expression in vitro. Our study demonstrates the need for carefully choosing the culture media and supplements due to the effect of steroid hormones.

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Regulation of Na+ absorption and Cl- secretion in the endometrium: switching mechanisms

Cited by 4 publications

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Altered cyclic expression of epithelial Na⁺ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet

Altered cyclic expression of epithelial Na⁺ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet

Endometrial gene expression in high- and low-fertility heifers in the late luteal phase of the estrous cycle and a comparison with midluteal gene expression

Effect of Phenol Red and Steroid Hormones on Cystic Fibrosis Transmembrane Conductance Regulator in Mouse Endometrial Epithelial Cells

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Regulation of Na+ absorption and Cl- secretion in the endometrium: switching mechanisms

Cited by 4 publications

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Altered cyclic expression of epithelial Na+ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet

Altered cyclic expression of epithelial Na+ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet

Endometrial gene expression in high- and low-fertility heifers in the late luteal phase of the estrous cycle and a comparison with midluteal gene expression

Effect of Phenol Red and Steroid Hormones on Cystic Fibrosis Transmembrane Conductance Regulator in Mouse Endometrial Epithelial Cells

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Altered cyclic expression of epithelial Na⁺ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet

Altered cyclic expression of epithelial Na⁺ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet