Regulation of murine placentogenesis by the retroviral genes Syncytin-A, Syncytin-B and Peg10

Henke, Christine; Ruebner, Matthias; Faschingbauer, Florian; Stolt, C. Claus; Schaefer, Natascha; Lang, Nadine; Beckmann, Matthias W.; Strissel, Pamela L.; Strick, Reiner

doi:10.1016/j.diff.2013.02.002

Cited by 24 publications

(28 citation statements)

References 44 publications

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“…Indeed, PEG3 can up‐regulate the production of autophagy‐related proteins such as Beclin1 and LC3 (Broad & Keverne, ), which are implicated in the etiology of intrauterine growth restriction (Hung et al, ) and preeclampsia (Gao et al, ). Peg10 is highly expressed in the mouse placenta, increasing from Days 8.5 to 18.5, with the highest expression in the labyrinth layer, particularly in the sinusoidal trophoblast giant cells, implying that PEG10 functions at the interface between the fetal and maternal blood systems (Henke et al, ). Peg10 deletion is known to cause early embryonic lethality due to defects in the placenta (Ono et al, ).…”

Section: Discussionmentioning

confidence: 99%

Gene expression and epigenetic aberrations in F1‐placentas fathered by obese males

Mitchell

Strick

Strissel

et al. 2017

Molecular Reproduction Devel

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Gene expression and/or epigenetic deregulation may have consequences for sperm and blastocysts, as well as for the placenta, together potentially contributing to problems observed in offspring. We previously demonstrated specific perturbations of fertilization, blastocyst formation, implantation, as well as aberrant glucose metabolism and adiposity in offspring using a mouse model of paternal obesity. The current investigation analyzed gene expression and methylation of specific CpG residues in F1 placentas of pregnancies fathered by obese and normal-weight male mice, using real-time PCR and bisulfite pyrosequencing. Our aim was to determine if paternal obesity deregulated placental gene expression and DNA methylation when compared to normal-weight males. Gene methylation of sperm DNA was analyzed and compared to placentas to address epigenetic transmission. Of the 10 paternally expressed genes (Pegs), 11 genes important for development and transport of nutrients, and the long-terminal repeat Intracisternal A particle (IAP) elements, derived from a member of the class II endogenous retroviral gene family, we observed a significant effect of paternal diet-induced obesity on deregulated expression of Peg3, Peg9, Peg10, and the nutrient transporter gene Slc38a2, and aberrant DNA methylation of the Peg9 promoter in F1 placental tissue. Epigenetic changes in Peg9 were also found in sperm from obese fathers. We therefore propose that paternal obesity renders changes in gene expression and/or methylation throughout the placental genome, which could contribute to the reproductive problems related to fertility and to the metabolic, long-term health impact on offspring.

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Section: Discussionmentioning

confidence: 99%

Gene expression and epigenetic aberrations in F1‐placentas fathered by obese males

Mitchell

Strick

Strissel

et al. 2017

Molecular Reproduction Devel

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“…Digoxigenin (DIG)‐labelled antisense and sense RNA probes were generated for Syncytin‐A and Syncytin‐B according to Henke et al . [42]. Plasmids were linearized with restriction enzymes and RNA probes were transcribed and DIG‐labelled using a RNA‐Labelling Kit (Roche, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Tissue sections were hybridized with Syncytin‐A and Syncytin‐B probes using ISH according to Henke et al . [42]. Lastly, ISH tissue sections were counterstained with nuclear fast red (Sigma‐Aldrich) and mounted in Mowiol (Roth, Germany).…”

Section: Methodsmentioning

confidence: 99%

Activation and regulation of endogenous retroviral genes in the human pituitary gland and related endocrine tumours

Buslei

Strissel

Henke

et al. 2015

Neuropathology Appl Neurobio

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“…Syncytin, a fusogenic glycoprotein of endogenous retroviral origin, plays a critical role in the regulation of cytotrophoblast differentiation [57]. Among the PPAR family, the role of PPARG in regulating syncytin-1 mRNA expression as well as trophoblast differentiation and invasion is well established [58,59].…”

Section: Ding Et Almentioning

confidence: 99%

Activation of Peroxisome Proliferator Activator Receptor Delta in Mouse Impacts Lipid Composition and Placental Development at Early Stage of Gestation1

Ding

Zhang

Liu³

et al. 2014

Biology of Reproduction

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Peroxisome proliferator-activated receptor delta (Ppard) activation has been implicated in regulating a multitude of biological processes in placental development. In this study, we employed the UPLC-ESI-TOFMS approach to investigate the metabolic traits in placenta from GW501516-treated mice at Embryonic Day 10.5. By analyzing the mass spectrum data, ions with the most significant differences between control and GW501516-treated groups were identified. Among these metabolites, the fatty acids, phospholipids, and sterol lipids were dramatically increased. Ingenuity Pathway Analysis (IPA) showed that phosphatidylethanolamine biosynthesis and glycolysis were the top two altered metabolic pathways involved in carbohydrate metabolism, energy production, and lipid metabolism. Subsequent immunoblotting experiments provided evidence for positive correlation of PPARD level and AKT and ERK signaling pathways upon GW501516 treatment. Furthermore, the stimulation of GW501516 increased trophoblast cell fusion gene syncytin-A (Syna), but not syncytin-B (Synb), expression, suggesting a potential role of Ppard activation in promoting cytotrophoblast differentiation. Our results reveal that Ppard activation elicits dramatic changes of the metabolic activities in placenta, which is correlated to AKT and ERK signaling.

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Regulation of murine placentogenesis by the retroviral genes Syncytin-A, Syncytin-B and Peg10

Cited by 24 publications

References 44 publications

Gene expression and epigenetic aberrations in F1‐placentas fathered by obese males

Gene expression and epigenetic aberrations in F1‐placentas fathered by obese males

Activation and regulation of endogenous retroviral genes in the human pituitary gland and related endocrine tumours

Activation of Peroxisome Proliferator Activator Receptor Delta in Mouse Impacts Lipid Composition and Placental Development at Early Stage of Gestation1

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