Regulation of mucin exocytosis from intestinal goblet cells

Laboisse, Christian L.; Jarry, Anne; Branka, Jean-Eric; Merlin, Didier; Bou-Hanna, Chantal; Vallette, G.

doi:10.1042/bst0230810

Cited by 20 publications

(20 citation statements)

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“…It has also been suggested that electrolyte secretion may provide the osmotic driving forces (140,261,262). There are two possible secretory pathways for secreted mucins in intestinal mucin-secreting cells (108,206,207). The first of these is the regular vesicular constitutive pathway of mucin exocytosis, also known as baseline secretion, in which no storage occurs, since the small vesicles transporting the mucins through the constitutive pathway are guided directly to the cell surface via microtubules and undergo immediate exocytosis of their contents.…”

Section: Mucinsmentioning

confidence: 99%

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The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

2006

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SUMMARY The intestinal tract is a complex ecosystem that combines resident microbiota and the cells of various phenotypes with complex metabolic activities that line the epithelial wall. The intestinal cells that make up the epithelium provide physical and chemical barriers that protect the host against the unwanted intrusion of microorganisms that hijack the cellular molecules and signaling pathways of the host and become pathogenic. Some of the organisms making up the intestinal microbiota also have microbicidal effects that contribute to the barrier against enteric pathogens. This review describes the two cell lineages present in the intestinal epithelium: the goblet cells and the Paneth cells, both of which play a pivotal role in the first line of enteric defense by producing mucus and antimicrobial peptides, respectively. We also analyze recent insights into the intestinal microbiota and the mechanisms by which some resident species act as a barrier to enteric pathogens. Moreover, this review examines whether the cells producing mucins or antimicrobial peptides and the resident microbiota act in partnership and whether they function individually and/or synergistically to provide the host with an effective front line of defense against harmful enteric pathogens.

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Section: Mucinsmentioning

confidence: 99%

“…The intestinal mucosa has a surface coating of mucus that is secreted by the specialized goblet cells, also known as mucinsecreting cells (72,108,207).…”

Section: Mucusmentioning

confidence: 99%

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

2006

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“…Both secreted and membrane-bound intestinal mucins have been characterized in HT29-MTX and HT29-FU cell subpopulations, since MUC2 and MUC4 were highly expressed in HT29-FU and MUC3 and MUC5AC were highly expressed in HT29-MTX (62)(63)(64)(65)(66)(67)(68). The mucin-secreting HT-29 cell clones and subpopulations have been used to investigate the regulation of mucus transport (69)(70)(71)(72)(73)(74)(75)(76)(77)(78) and the functionality of human intestinal mucin-secreting cells (26,27). Moreover, mucin-secreting HT-29 cell subpopulations and clones have been used to investigate the role of human mucins in bacterial pathogenesis (62,63,(79)(80)(81).…”

Section: Differentiated Mucin-secreting Ht-29 Cell Subpopulations Andmentioning

confidence: 99%

“…The intracellular processing of mucins involves synthesis, oligomerization in the endoplasmic reticulum, glycosylation in the cis-and trans-Golgi networks, and storage in granules ( Fig. 1) (3,26,27). Intracellular small and large vesicles package the mucins, and the viscous mucus contained in vesicles is extruded after fusion of the vesicles and plasma membranes and the formation of a fusion pore through a process requiring an expulsive force (Fig.…”

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confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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SUMMARY Hosts are protected from attack by potentially harmful enteric microorganisms, viruses, and parasites by the polarized fully differentiated epithelial cells that make up the epithelium, providing a physical and functional barrier. Enterovirulent bacteria interact with the epithelial polarized cells lining the intestinal barrier, and some invade the cells. A better understanding of the cross talk between enterovirulent bacteria and the polarized intestinal cells has resulted in the identification of essential enterovirulent bacterial structures and virulence gene products playing pivotal roles in pathogenesis. Cultured animal cell lines and cultured human nonintestinal, undifferentiated epithelial cells have been extensively used for understanding the mechanisms by which some human enterovirulent bacteria induce intestinal disorders. Human colon carcinoma cell lines which are able to express in culture the functional and structural characteristics of mature enterocytes and goblet cells have been established, mimicking structurally and functionally an intestinal epithelial barrier. Moreover, Caco-2-derived M-like cells have been established, mimicking the bacterial capture property of M cells of Peyer's patches. This review intends to analyze the cellular and molecular mechanisms of pathogenesis of human enterovirulent bacteria observed in infected cultured human colon carcinoma enterocyte-like HT-29 subpopulations, enterocyte-like Caco-2 and clone cells, the colonic T84 cell line, HT-29 mucus-secreting cell subpopulations, and Caco-2-derived M-like cells, including cell association, cell entry, intracellular lifestyle, structural lesions at the brush border, functional lesions in enterocytes and goblet cells, functional and structural lesions at the junctional domain, and host cellular defense responses.

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“…Eight genes (MUC1, MUC3A, MUC3B, MUC4, MUC12, MUC13, MUC16, and MUC17) that code for membrane-associated mucins have been characterized. In mucin-secreting intestinal cells, the secreted mucins are packaged and stored into large intracellular vesicles (17,39).…”

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confidence: 99%

Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells

et al. 2010

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In rabbit ligated ileal loops, two atypical enteropathogenic Escherichia coli (aEPEC) strains, 3991-1 and 0421-1, intimately associated with the cell membrane, forming the characteristic EPEC attachment and effacement lesion of the brush border, induced a mucous hypersecretion, whereas typical EPEC (tEPEC) strain E2348/69 did not. Using cultured human mucin-secreting intestinal HT29-MTX cells, we demonstrate that apically aEPEC infection is followed by increased production of secreted MUC2 and MUC5AC mucins and membrane-bound MUC3 and MUC4 mucins. The transcription of the MUC5AC and MUC4 genes was transiently upregulated after aEPEC infection. We provide evidence that the apically adhering aEPEC cells exploit the mucins' increased production since they grew in the presence of membrane-bound mucins, whereas tEPEC did not. The data described herein report a putative new virulence phenomenon in aEPEC.The intestinal mucus offers numerous ecological advantages to bacteria present in the lumen and intestinal epithelium, providing a source of energy by producing the saccharides used for sustained growth by both the indigenous enteric microbiota and pathogens (26). Moreover by producing mucus, goblet cells contribute to the physical and chemical barriers that protect the host against the unwanted intrusion of enterovirulent microorganisms (48). Currently, 17 human mucin-type glycoproteins have been assigned to the MUC gene family, MUC1 and -2, MUC3A, MUC3B, MUC4, MUC5B, MUC5AC,, with the approval of the Human Genome Organization Gene Nomenclature Committee (HUGO/GNC; http://www.hugo-international.org/hugo/) (8, 9, 57). A cluster of four mucin genes (MUC2, MUC5B, MUC5AC, and MUC6) code for secreted mucins. Eight genes (MUC1, MUC3A, MUC3B, MUC4, MUC12, MUC13, MUC16, and MUC17) that code for membrane-associated mucins have been characterized. In mucin-secreting intestinal cells, the secreted mucins are packaged and stored into large intracellular vesicles (17, 39).Pathogenic Escherichia coli causes intestinal and extraintestinal diseases (35). There are six well-defined categories of intestinal pathogenic E. coli, each characterized by specific virulence factors that affect a wide range of cellular processes via signaling events. Enteropathogenic E. coli (EPEC), the first pathotype of E. coli to be described, produces characteristic cellular lesions known as "attaching and effacing" (A/E) lesions in the intestinal mucosa after the bacteria have intimately attached to the enterocyte brush border membrane and caused cytoskeletal changes that lead to effacement of the microvilli. The EPEC group is subdivided into typical (tEPEC) and atypical (aEPEC) forms. The aEPEC group consists of a large number of O serogroups (29, 74). These strains have been shown to carry the locus of the enterocyte effacement (LEE) pathogenicity island (PAI), but to lack the EAF (EPEC adherence factor) plasmid that encodes the bundle-forming pilus (BFP) and the Shiga toxin genes (35). LEE encodes the components of a type 3 secretion system (T3SS), an ...

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Regulation of mucin exocytosis from intestinal goblet cells

Cited by 20 publications

References 1 publication

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells

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