Regulation of mitochondrial biosynthesis and function by dietary carbohydrate levels and lipid sources in juvenile blunt snout bream Megalobrama amblycephala

Li, Xiangfei; Wang, Bingke; Xu, Chao; Shi, Haojie; Zhang, Li; Liu, Jia-Dai; Tian, Hongyan; Liu, Wenbin

doi:10.1016/j.cbpa.2018.08.008

Cited by 11 publications

(6 citation statements)

References 58 publications

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“…The influence of nutrient levels on the gene expression of respiratory chain uncoupling proteins, UCP2 and UCP3, is well documented in rainbow trout (Coulibaly et al, 2006) and gilthead seabream (Bermejo-Nogales et al, 2011, with these being the first to be modified in response to both environmental and nutritional (by caloric restriction) stressors (Bermejo-Nogales et al, 2014). Diet can modulate fusion, fission, biogenesis, and oxidation processes in yellow catfish (Song et al, 2020) and snout bream (Li et al, 2019). The ratio of cytochrome c oxidase (COX) to citrate synthase (CS) mitochondrial enzyme activity in the white muscle of exercised gilthead seabream is modulated differently depending on diet and life stage, with more protein (Blasco et al, 2015) and more carbohydrates (Martin-Perez et al, 2012) in fingerlings and juveniles, respectively (Martin-Perez et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Adaptation to Diet and Swimming Activity in Gilthead Seabream: Improved Nutritional Efficiency

et al. 2021

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Sustained exercise promotes growth in different fish species, and in gilthead seabream we have demonstrated that it improves nutrient use efficiency. This study assesses for differences in growth rate, tissue composition and energy metabolism in gilthead seabream juveniles fed two diets: high-protein (HP; 54% protein, 15% lipid) or high energy (HE; 50% protein, 20% lipid), under voluntary swimming (VS) or moderate-to-low-intensity sustained swimming (SS) for 6 weeks. HE fed fish under VS conditions showed lower body weight and higher muscle lipid content than HP fed fish, but no differences between the two groups were observed under SS conditions. Irrespective of the swimming regime, the white muscle stable isotopes profile of the HE group revealed increased nitrogen and carbon turnovers. Nitrogen fractionation increased in the HP fed fish under SS, indicating enhanced dietary protein oxidation. Hepatic gene expression markers of energy metabolism and mitochondrial biogenesis showed clear differences between the two diets under VS: a significant shift in the COX/CS ratio, modifications in UCPs, and downregulation of PGC1a in the HE-fed fish. Swimming induced mitochondrial remodeling through upregulation of fusion and fission markers, and removing almost all the differences observed under VS. In the HE-fed fish, white skeletal muscle benefited from the increased energy demand, amending the oxidative uncoupling produced under the VS condition by an excess of lipids and the pro-fission state observed in mitochondria. Contrarily, red muscle revealed more tolerant to the energy content of the HE diet, even under VS conditions, with higher expression of oxidative enzymes (COX and CS) without any sign of mitochondrial stress or mitochondrial biogenesis induction. Furthermore, this tissue had enough plasticity to shift its metabolism under higher energy demand (SS), again equalizing the differences observed between diets under VS condition. Globally, the balance between dietary nutrients affects mitochondrial regulation due to their use as energy fuels, but exercise corrects imbalances allowing practical diets with lower protein and higher lipid content without detrimental effects.

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Section: Introductionmentioning

confidence: 99%

Mitochondrial Adaptation to Diet and Swimming Activity in Gilthead Seabream: Improved Nutritional Efficiency

et al. 2021

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“…The lysosome contains a variety of acid hydrolases, it can defend and keep normal metabolism process in cells, and control the transportation of metabolism product, ion and hydrolase[52, 53]. The function of lysosomes is to degrade intracellular molecules (autophagy), as well as extracellular content (heterophagy), in which case there might be a crosstalk between physiology and immunity, such as the decreased metabolism and cellular damage responses under stress condition [31]. In this study, the lysosome pathway and cold stress responsive genes were significantly enriched and identified in ST.versus.SC based on the KEGG analysis, indicating the cellular damage in the non‐adapted southern population when suffering cold stress.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, iTRAQ (Isobaric Tag for Relative Absolute Quantitation) as a high throughput proteomics experimental approach that has been widely applied to identify the proteomic profile and stress-responsive proteins in different aquatic animals [19,30]. So far, it has been widely used in the research of shellfish protein alterations, such as environmental stress [31,32], heavy metals [33,34],…”

Section: Introductionmentioning

confidence: 99%

iTRAQ‐based proteomic analysis reveals proteins associated with cold stress response in two different populations of Manila clam (Ruditapes philippinarum)

Nie

Dong

et al. 2023

Proteomics

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Water temperature is one of the key environmental factors for marine ectotherms and a change in temperature beyond and organism's capacity limits can cause a series of changes to physiological state and damage to the organism. Understanding how organisms adapt to complex environments is a central goal of evolutionary biology and ecology. Ruditapes philippinarum is an ecologically and scientifically important marine bivalve species. To uncover the molecular mechanisms of acclimation of R. philippinarum to low-temperature stress, iTRAQ-based quantitative proteomics was conducted to compare the proteomes of the north and south populations of R. philippinarum under low-temperature stress. The results showed a total of 6355 and 6352 proteins were identified in two populations, respectively. Among these, 94 and 83 were differentially abundant proteins (DAPs), and most of DAPs were related to oxidationprocess, protein binding, or an integral component of membrane. According to the results of KEGG pathway enrichment analysis, most of DAPs in both populations are involved in immune-related pathways, while other population-specific significant abundance proteins of south population and north population were enriched in biosynthesis of amino acids (Enolase, Glutamine synthetase) and unsaturated fatty acids pathways (3-ketoacyl-CoA thiolase, Stearoyl-CoA desaturase), respectively, indicating that two population of clams may have different cold-stress regulation mechanisms.Our study provides new insights into different cold stress tolerance mechanisms in northern and southern populations of R. philippinarum using iTRAQ-based proteomics. This work contributes to a better understanding of molecular basis on cold stress response and adaptations, which shed lights on evolutionary biology and general ecophysiology of R. philippinarum.

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“…At present, diets formulated for this species commonly contain relatively high concentrations of carbohydrates. However, reduced growth rates, metabolic disorders and compromised health status have been reported and linked to dietary carbohydrate concentrations (8,22) . This study explored the interactive effects of dietary carbohydrate levels and thiamine supplementation on growth performance, feed utilization, and intestinal mitochondrial biogenesis and function in bream.…”

Section: Blunt Snout Bream (Megalobrama Amblycephala) Is An Importantmentioning

confidence: 99%

“…All cells need adenosine triphosphate (ATP) as a source of energy (6) , while mitochondria are the principal organelles producing ATP (7) . A previous study reported that impaired mitochondrial biogenesis and function would result in oxidative stress of fish and could consequently inhibit the mitochondrial respiratory chain complex activities and ATP production, thus impairing nutrient metabolism (8) . Hence, maintaining the normal intestinal mitochondrial function is of great significance, as it may facilitate non-protein energy utilisation.…”

mentioning

confidence: 99%

Interactions between dietary carbohydrate and thiamine: implications on the growth performance and intestinal mitochondrial biogenesis and function of Megalobrama amblycephala

You

Brown

et al. 2021

Br J Nutr

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A 12-week experiment was conducted to evaluate the influences of thiamine on growth performance and intestinal mitochondrial biogenesis and function of M. amblycephala fed a high-carbohydrate (HC) diet. Fish (24.73 ± 0.45 g) were randomly assigned one of four diets; two carbohydrate levels (30 and 45%) and two thiamine levels (0 and 1.5 mg kg-1). HC diets significantly decreased DGC, GRMBW, FIMBW, intestinal activities of amylase, lipase, Na+, K+-ATPase, CK, complex I, III and IV, intestinal microvilli length, number of mitochondrial per field, ΔΨm, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, TFAM, Opa-1, ND-1 and COX-1 and 2, while the opposite was true for ATP, AMP and ROS, and the transcriptions of Drp-1, Fis-1 and Mff. Dietary thiamine concentrations significantly increased DGC, GRMBW, intestinal activities of amylase, Na+, K+-ATPase, CK, complex I and IV, intestinal microvilli length, number of mitochondrial per field, ΔΨm, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, Opa-1, ND-1, COX-1 and 2, SGLT-1 and GLUT-2. Furthermore, a significant interaction between dietary carbohydrate and thiamine was observed in DGC, GRMBW, intestinal activities of amylase, CK, complex I and IV, ΔΨm, the AMP/ATP ratio, the P-AMPK/T-AMPK ratio, PGC-1β protein expression as well as the transcriptions of AMPKα1, AMPKα2, PGC-1β, Opa-1, COX-1 and 2, SGLT-1 and GLUT-2. Overall, thiamine supplementation improved growth performance, and intestinal mitochondrial biogenesis and function of M. amblycephala fed HC diets.

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Regulation of mitochondrial biosynthesis and function by dietary carbohydrate levels and lipid sources in juvenile blunt snout bream Megalobrama amblycephala

Cited by 11 publications

References 58 publications

Mitochondrial Adaptation to Diet and Swimming Activity in Gilthead Seabream: Improved Nutritional Efficiency

Mitochondrial Adaptation to Diet and Swimming Activity in Gilthead Seabream: Improved Nutritional Efficiency

iTRAQ‐based proteomic analysis reveals proteins associated with cold stress response in two different populations of Manila clam (Ruditapes philippinarum)

Interactions between dietary carbohydrate and thiamine: implications on the growth performance and intestinal mitochondrial biogenesis and function of Megalobrama amblycephala

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