Regulation of microtubule dynamics by the neuronal growth-associated protein SCG10

Riederer, Beat M.; Pellier, Véronique; Antonsson, Bruno; Paolo, Gilbert Di; Stimpson, Stephen A.; Lütjens, Robert; Catsicas, Stefan; Grenningloh, Gabriele

doi:10.1073/pnas.94.2.741

Cited by 181 publications

(195 citation statements)

References 39 publications

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“…We further examine the expressions of RhoA (neurite extension [43]) and PC3 (neuronal birth [44]), resulting in no clear differences of their expression levels between the adult and embryonic NP cells. Expression of SCG10, enriched in the growth cones of the developing neurons [45], was rather decreased in the SVZ and WM cultures compared with that of E14 NP cells. Additional studies are required for an understanding of the molecular basis for the difference of the Nurr1 effects between adult and embryonic NP cells.…”

Section: Discussionmentioning

confidence: 96%

Generation of Functional Dopamine Neurons from Neural Precursor Cells Isolated from the Subventricular Zone and White Matter of the Adult Rat Brain Using Nurr1 Overexpression

et al. 2007

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Section: Discussionmentioning

confidence: 96%

Generation of Functional Dopamine Neurons from Neural Precursor Cells Isolated from the Subventricular Zone and White Matter of the Adult Rat Brain Using Nurr1 Overexpression

et al. 2007

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“…These in vitro and in vivo observations are consistent with a role for exo70, and thus exocyst, in modulating microtubule dynamics underlying plasma membrane addition. In this regard, it is interesting to note that in neuroendocrine PC12 cells the exocyst has a similar intracellular localization as that of the microtubule-destabilizing protein SCG10 (24). Both macromolecules are enriched in the growth cone where high levels of microtubule ends are present.…”

Section: Discussionmentioning

confidence: 98%

The Mammalian Exocyst, a Complex Required for Exocytosis, Inhibits Tubulin Polymerization

Wang

Liu

Adamson

et al. 2004

Journal of Biological Chemistry

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The exocyst is a 734-kDa complex essential for development. Perturbation of its function results in early embryonic lethality. Extensive investigation has revealed that this complex participates in multiple biological processes, including protein synthesis and vesicle/ protein targeting to the plasma membrane. In this article we report that the exocyst may also play a role in modulating microtubule dynamics. Using monoclonal antibodies, we observed that endogenous exocyst subunits co-localized with microtubules and mitotic spindles in normal rat kidney cells. To test for a functional relationship between the exocyst complex and microtubules, we established an in vitro exocyst reconstitution assay and studied exocyst effect on microtubule dynamics. We found that the exocyst complex reconstituted from eight recombinant exocyst subunits inhibited tubulin polymerization in vitro. Deletion of exocyst subunit sec5, sec6, sec15, or exo70 diminished its tubulin polymerization inhibition activity. Surprisingly, exocyst subunit exo70 itself was also capable of inhibiting tubulin polymerization, although exocyst complex with exo70 deletion did not lose its activity completely. Overexpression of exo70 in NRK cells resulted in microtubule network disruption and the formation of filopodia-like plasma membrane protrusions. The formation of these membrane protrusions was greatly hampered by stabilizing microtubules with taxol. Overexpression of exo84, an exocyst subunit that did not show tubulin polymerization inhibition activity, did not cause this phenotype. Results shown in this article, along with a previous report that localized microtubule instability induces plasma membrane addition, implicates a novel role for the exocyst in modulating microtubule dynamics underlying exocytosis.

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“…In our study, the growth-associated expression and heterogeneous distribution of nKHC appear to be strikingly similar to the ones of GAP 43 and SCGIO, suggesting that nKHC may indeed play a specific role in microtubule-based transport during axon growth and refinement of synaptic connections. In this respect, it is interesting that both GAP 43 and SCG1O have been shown to interact with and regulate the dynamics of cytoskeletal components, such as actin filaments and microtubules, respectively (Hens et al, 1993;Riederer et al, 1997).…”

Section: Figmentioning

confidence: 99%

Expression of Neuronal Kinesin Heavy Chain Is Developmentally Regulated in the Central Nervous System of the Rat

Vignali

Lizier²,

Sprocati

et al. 1997

Journal of Neurochemistry

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Abstract:The kinesin family of motor proteins comprises at least two isoforms of conventional kinesin encoded by different genes: ubiquitous kinesin, expressed in all cells and tissues, and neuronal kinesin, expressed exclusively in neuronal cells. In the present study, we have analyzed the expression of the two kinesin isoforms by immunochemistry at different stages of development of the rat CNS. We have found that the level of expression of neuronal kinesin is five to eight times higher in developing than in adult rat brains, whereas that of ubiquitous kinesin is only~2.5 times higher in maturing versus adult brains. Moreover, we have studied the distribution of neuronal kinesin by light microscopic immunocytochemistry in the rat brain at different postnatal ages and have found this protein not only to be more highly expressed in juvenile than in adult rat brains but also to show a different pattern of distribution. In particular, tracts of axonal fibers were clearly stained at early postnatal stages of development but were markedly unlabeled in adult rat brains. Our results indicate that the expression of at least one isoform of conventional neuron-specific kinesin is up-regulated in the developing rat CNS and suggest that this protein might play an important role in microtubule-based transport during the maturation of neuronal cells in vivo. Key Words: Microtubule-based motors-Conventional kinesin isoforms-Quantitative protein expression-Immunocytochemical localization-Brain development.

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Regulation of microtubule dynamics by the neuronal growth-associated protein SCG10

Cited by 181 publications

References 39 publications

Generation of Functional Dopamine Neurons from Neural Precursor Cells Isolated from the Subventricular Zone and White Matter of the Adult Rat Brain Using Nurr1 Overexpression

Generation of Functional Dopamine Neurons from Neural Precursor Cells Isolated from the Subventricular Zone and White Matter of the Adult Rat Brain Using Nurr1 Overexpression

The Mammalian Exocyst, a Complex Required for Exocytosis, Inhibits Tubulin Polymerization

Expression of Neuronal Kinesin Heavy Chain Is Developmentally Regulated in the Central Nervous System of the Rat

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