Regulation of MicroRNA-155 in Atherosclerotic Inflammatory Responses by Targeting MAP3K10

Abstract

AimsAccumulating evidence suggest that numerous microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and differentiation, as well as various diseases that accompany inflammatory responses. Inflammation is known to be a major contributor to atherogenesis. Previous studies provide promising evidence in support of the role of miRNAs in cardiovascular disease. However, mechanistic data on these small molecules in atherosclerosis (AS) are still missing. The present study aims to investigate the potential role of miRNAs in AS.Methods and ResultsThe miRNA transcriptase was verified by TaqMan real-time polymerase chain reaction assay. Thoracic aorta samples were obtained from Apolipoprotein E knockout mice, and plasma samples were from coronary artery disease (CAD) patients. The results showed that the miR-155 level was the most significantly elevated both in AS mice and CAD patients relative to the normal control. The functional role of miR-155 in the atherosclerotic path physiological process was also observed in vivo and in vitro. The observations suggested that miR-155 is a part of a negative feedback loop, which down-modulates inflammatory cytokine production and decreases AS progression. miR-155 was also found to mediate the inflammatory response and mitogen-activated protein kinase (MAPK) pathway by targeting mitogen-activated protein kinase kinase kinase 10.ConclusionsmiR-155 contributes to the prevention of AS development and progression. It may also be involved in the posttranscriptional regulation of the inflammatory response and MAPK pathway by targeting mitogen-activated protein kinase kinase kinase 10.

Upregulation of miRNA-155 expression by OxLDL in dendritic cells involves JAK1/2 kinase and transcription factors YY1 and MYB

“…For instance, Nazari-Jahantigh et al (17) and other researchers have reported that miR-155 promotes the development of AS (18). On the other hand, research from our laboratory suggests that miR-155 plays a protective role in the development and progression of AS (19). In a murine model of hyperlipidemia, miR-155 was found to act as an anti-inflammatory and protective factor against AS (20).…”

“…Interestingly, miR-155 has also been found to be involved in cardiovascular disease: overexpression of this miRNA-155 was accompanied by impaired angiotensin II type 1 receptor (AT1R) activity and low blood pressure (31). Our previous research has shown that miR-155 contributed to the prevention of AS development and progression (19). Although previous research has demonstrated the role of miR-155 in multiple diseases, relatively little is known about how its expression is mediated in a disease setting, particularly in an immuno-inflammatory context.…”

Blockade of 146b‑5p promotes inflammation in atherosclerosis‑associated foam cell formation by targeting TRAF6

“…All cells were incubated in a humidified atmosphere with 5% CO 2 at 37˚C. A foam cell model was established as previously described (14)(15)(16). In brief, THP-1 cells were first seeded in culture plates at 1x10 6 cells/ml with 100 nM phorbol 12-myristate 13-acetate (PMA; Sangon Biotech Co., Ltd., Shanghai, China) for 12 h to differentiate them into macrophages.…”

MicroRNA 224 Regulates Ion Transporter Expression in Ameloblasts To Coordinate Enamel Mineralization

“…This allows us to determine the effect of miR-224 on ameloblast differentiation postnatally. MicroRNA agomir is a chemically modified oligonucleotide widely applied in microRNA investigation (56,57). Injection of miR-224 agomir resulted in abnormal ameloblast morphology and decreased target ion transporter expression.…”