Regulation of Mesenchymal Stem Cell Differentiation by Nanopatterning of Bulk Metallic Glass

Abstract

Mesenchymal stem cell (MSC) differentiation is regulated by surface modification including texturing, which is applied to materials to enhance tissue integration. Here, we used Pt57.5Cu14.7Ni5.3P22.5 bulk metallic glass (Pt-BMG) with nanopatterned surfaces achieved by thermoplastic forming to influence differentiation of human MSCs. Pt-BMGs are a unique class of amorphous metals with high strength, elasticity, corrosion resistance, and an unusual plastic-like processability. It was found that flat and nanopattened Pt-BMGs induced osteogenic and adipogenic differentiation, respectively. In addition, osteogenic differentiation on flat BMG exceeded that observed on medical grade titanium and was associated with increased formation of focal adhesions and YAP nuclear localization. In contrast, cells on nanopatterned BMGs exhibited rounded morphology, formed less focal adhesions and had mostly cytoplasmic YAP. These changes were preserved on nanopatterns made of nanorods with increased stiffness due to shorter aspect ratios, suggesting that MSC differentiation was primarily influenced by topography. These observations indicate that both elemental composition and nanotopography can modulate biochemical cues and influence MSCs. Moreover, the processability and highly tunable nature of Pt-BMGs enables the creation of a wide range of surface topographies that can be reproducibly and systematically studied, leading to the development of implants capable of engineering MSC functions.

Interaction of Human Mesenchymal Stem Cells with Soft Nanocomposite Hydrogels Based on Polyethylene Glycol and Dendritic Polyglycerol

“…hMSCs are involved in numerous treatments in various diseases due notably to their differentiation potential . We focus especially here on the adipogenesis potential for reconstructive medicine to cite only one example.…”

<p>Efficient Labeling Of Mesenchymal Stem Cells For High Sensitivity Long-Term MRI Monitoring In Live Mice Brains</p>

“…One important property of bone marrow-derived mesenchymal stem cells is to differentiate into different specialized cell types such as osteoblasts and adipocytes. 28,29 Along with the capability to produce growth factors, mesenchymal stem cells need their capability of differentiating into different cell types to boost regeneration of injured tissues, 30 and hence exert their therapeutic role. In order to determine whether labeling our mesenchymal stem cells with IO-MI NPs will affect their differentiation capabilities, we performed osteogenic and adipogenic differentiation experiments for labeled and nonlabeled FGF21 MSCs ( Figure 4A) and mCherry MSCs ( Figure 4B).…”

Microtopography based on inverse opal structures regulates the behavior of bone marrow‐derived mesenchymal stem cells

“…Studies have shown that substrates with microstructured or nanostructured surfaces could strongly influence the fate of MSCs . Dalby and his colleagues reported that substrates with deliberately disordered pits in a square arrangement (±50‐nm offset) induced MSCs to differentiate into osteoblasts, while nanotopography with pits arranged in absolute square lattice symmetry retained stem‐cell phenotype and maintained stem‐cell growth of MSCs over 8 weeks .…”

“…[8][9][10][11][12] Studies have shown that substrates with microstructured or nanostructured surfaces could strongly influence the fate of MSCs. [13][14][15] Dalby and his colleagues reported that substrates with deliberately disordered pits in a square arrangement (±50-nm offset) induced MSCs to differentiate into osteoblasts, 16 while nanotopography with pits arranged in absolute square lattice symmetry retained stem-cell phenotype and maintained stem-cell growth of MSCs over 8 weeks. 17 Interestingly, it was found that PHBV electrospun nanofibrous scaffolds loaded with hydroxyapatite nanoparticles could induce spontaneous osteogenic differentiation of bone marrow-derived MSCs in the absence of osteogenic inducing media.…”