Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages

Fiani, Maria L.; Beitz, Jill; Turvy, Diane N.; Blum, Janice S.; Stahl, Philip D.

doi:10.1002/jlb.64.1.85

Cited by 30 publications

(25 citation statements)

References 36 publications

(34 reference statements)

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“…J774-33 cells were used in this study since they readily bind and phagocytose C. perfringens and other Gram-positive bacteria (O9Brien & Melville, 2000). J774 cells are known to express the scavenger receptor (Ding et al, 1998) and the Fc receptor (Martin & Weis, 1993) and some clones express the mannose receptor (Fiani et al, 1998). They also express the complement receptor CR3 (Hall et al, 1991), but not CR1 or CR2 (Martin & Weis, 1993).…”

Section: Discussionmentioning

confidence: 99%

Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors

O’Brien

Melville

2003

Microbiology

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Clostridium perfringens is a Gram-positive, anaerobic bacterium that is the most common cause of gas gangrene (clostridial myonecrosis) in humans. C. perfringens produces a variety of extracellular toxins that are thought to be the major virulence factors of the organism. However, C. perfringens has recently been shown to have the ability to survive in a murine macrophage-like cell line, J774-33, even under aerobic conditions. In J774-33 cells, C. perfringens can escape the phagosome and gain access to the cytoplasm. Since the receptor that is used for phagocytosis can determine the fate of an intracellular bacterium, we used a variety of inhibitors of specific receptors to identify those used by J774-33 cells to phagocytose C. perfringens. It was found that the scavenger receptor and mannose receptor(s) were involved in the phagocytosis of C. perfringens. In the presence of complement, the complement receptor (CR3) was also involved in the binding and/or uptake of C. perfringens. Since the receptor inhibition studies indicated that the scavenger receptor played a major role in phagocytosis, C. perfringens binding studies were performed with a Chinese hamster ovary (CHO) cell line expressing the mouse SR-A receptor. The cell line expressing the SR-A receptor showed a significant increase in C. perfringens binding in comparison to the non-transfected CHO cells. In the absence of opsonizing antibodies, the Fc receptor was not used to phagocytose C. perfringens. Forcing the macrophages to use a specific receptor by using combinations of different receptor inhibitors led to only a slight increase in co-localization of intracellular C. perfringens with the late endosome-lysosome marker LAMP-1. Carbohydrate analysis of C. perfringens strain 13 extracellular polysaccharide confirmed the presence of mannose and negatively charged residues of glucuronic acid, which may provide the moieties that promote binding to the mannose and scavenger receptors, respectively.

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Section: Discussionmentioning

confidence: 99%

Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors

O’Brien

Melville

2003

Microbiology

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“…We also determined whether expression of MR in J774 cells was sufficient to enhance Ft uptake. Indeed, MRpositive J774-E cells [23] ingested threefold more Ft than MR-negative J774 (Fig. 2C, P<0.01), and this enhanced infection was ablated by 0.1 mg/ml mannan (33±15% of no mannan J774-E control, n=3).…”

Section: Role For the Macrophage Mr In Ft Phagocytosismentioning

confidence: 91%

“…After 2 h at 37°C, monocyte monolayers were washed twice to remove nonadherent lymphocytes. J774 cells (obtained from the American Type Culture Collection, Manassas, VA) and MR-positive J774-E clone cells [23] (obtained from Philip D. Stahl, Washington University, St. Louis, MO) were maintained in DMEM containing 10% heat-inactivated (HI) FBS and 2 mM L-glutamine.…”

Section: Mononuclear Cell Isolation and Culturementioning

confidence: 99%

Differential infection of mononuclear phagocytes by Francisella tularensis: role of the macrophage mannose receptor

Schulert

Allen

2006

Journal of Leukocyte Biology

132

176

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Francisella tularensis (Ft) is a Gram-negative bacterium and the causative agent of tularemia. It is well established that this organism replicates inside macrophages, but we are only beginning to understand this interface at the molecular level. Herein, we compared directly the ability of Ft subspecies holarctica live-vaccine strain to infect freshly isolated human peripheral blood monocytes, monocyte-derived macrophages (MDM), and cells of the murine macrophage cell line J774A.1 (J774). We now show that unopsonized bacteria infected human MDM fivefold more efficiently than monocytes or J774 cells in standard media. Moreover, enhanced infection of MDM was mediated, in part, by the macrophage mannose receptor (MR). Forming Ft phagosomes accumulated MR, and infection was inhibited by MR-blocking antibody or soluble mannan but not by the dectin-1 ligand laminarin. Up-regulation of MR in MDM (by exposure to interleukin-4) increased Ft phagocytosis, as did expression of MR in J774 cells. Conversely, opsonized Ft were ingested readily by monocytes and MDM. Medium supplementation with 2.5% fresh autologous serum was sufficient to confer opsonophagocytosis and CD11b accumulated in the membrane at sites of Ft engulfment. Infection of monocytes by opsonized Ft was nearly ablated by complement receptor 3 (CR3) blockade. Conversely, MDM used MR and CD11b/CD18 to ingest opsonized organisms. Altogether, our data demonstrate differential infection of mononuclear phagocytes by Ft and define distinct roles for MR and CR3 in phagocytosis.

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“…This is consistent with an expected increase in receptor production following CD206 binding events and activation that contributes to the rapid recycling of this receptor. 53,54 To assess the potential for delivery of IκBα siRNA by MnNPs to induce immunostimulatory and antitumor effects, mRNA from transfected BMDMs was analyzed by qRT-PCR. mRNA levels for IκBα are significantly decreased following transfection with IκBα siRNA, indicating successful knockdown at the mRNA level of IκBα ( Figure 8A).…”

mentioning

confidence: 99%

Manipulating the NF-κB pathway in macrophages using mannosylated, siRNA-delivering nanoparticles can induce immunostimulatory and tumor cytotoxic functions

Ortega¹,

Barham

Sharman

et al. 2016

IJN

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Tumor-associated macrophages (TAMs) are critically important in the context of solid tumor progression. Counterintuitively, these host immune cells can often support tumor cells along the path from primary tumor to metastatic colonization and growth. Thus, the ability to transform protumor TAMs into antitumor, immune-reactive macrophages would have significant therapeutic potential. However, in order to achieve these effects, two major hurdles would need to be overcome: development of a methodology to specifically target macrophages and increased knowledge of the optimal targets for cell-signaling modulation. This study addresses both of these obstacles and furthers the development of a therapeutic agent based on this strategy. Using ex vivo macrophages in culture, the efficacy of mannosylated nanoparticles to deliver small interfering RNA specifically to TAMs and modify signaling pathways is characterized. Then, selective small interfering RNA delivery is tested for the ability to inhibit gene targets within the canonical or alternative nuclear factor-kappaB pathways and result in antitumor phenotypes. Results confirm that the mannosylated nanoparticle approach can be used to modulate signaling within macrophages. We also identify appropriate gene targets in critical regulatory pathways. These findings represent an important advance toward the development of a novel cancer therapy that would minimize side effects because of the targeted nature of the intervention and that has rapid translational potential.

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Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages

Cited by 30 publications

References 36 publications

Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors

Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors

Differential infection of mononuclear phagocytes by Francisella tularensis: role of the macrophage mannose receptor

Manipulating the NF-κB pathway in macrophages using mannosylated, siRNA-delivering nanoparticles can induce immunostimulatory and tumor cytotoxic functions

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Regulation of mannose receptor synthesis and turnover in mouse J774 macrophages

Cited by 30 publications

References 36 publications

Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors

Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors

Differential infection of mononuclear phagocytes by Francisella tularensis: role of the macrophage mannose receptor

Manipulating the NF-&kappa;B pathway in macrophages using mannosylated, siRNA-delivering nanoparticles can induce immunostimulatory and tumor cytotoxic functions

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Manipulating the NF-κB pathway in macrophages using mannosylated, siRNA-delivering nanoparticles can induce immunostimulatory and tumor cytotoxic functions