Regulation of mammalian protein synthesis <i>in vivo</i>. Stimulated liver ribonucleic acid synthesis <i>in vivo</i> after cycloheximide treatment

Ch'ih, John J.; Pike, Lee M.; Devlin, Thomas M.

doi:10.1042/bj1680057

Cited by 24 publications

(11 citation statements)

References 31 publications

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“…To determine the effect of cycloheximide treatment (61–64) on protein synthesis in the brain, 2 h after the first injection, three cycloheximide‐ and three vehicle‐pretreated rats received an intravenous injection of L‐[ 35 S] Methionin (1 mCi in saline per animal) and were allowed to survive 3 h.…”

Section: Methodsmentioning

confidence: 99%

Activated Somatostatin Type 2 Receptors Traffic In Vivo in Central Neurons from Dendrites to the Trans Golgi Before Recycling

Lelouvier

Ghouzzi

Toyama

et al. 2007

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†These authors contributed equally to this work.Understanding the trafficking of G-protein-coupled receptors (GPCRs) is of particular importance, especially when modifications of the neurochemic environment occur as in pathological or therapeutic circumstances. In the central nervous system, although some GPCRs were reported to internalize in vivo, little is known about their trafficking downstream of the endocytic event. To address this issue, distribution and expression pattern of the major somatostatin receptor subtype, the somatostatin type 2 (sst2), was monitored in the hippocampus using immunofluorescence, autoradiographic and immunogold experiments from 10 minutes to 7 days after in vivo injection of the receptor agonist octreotide. We then analyzed whether postendocytic trafficking of the receptor was dependent upon integrity of the microtubule network using colchicine-injected animals. Together, our results suggest that upon agonist stimulation, dendritic receptors are retrogradely transported through a microtubule-dependent mechanism to a trans Golgi domain enriched in the t-SNARE syntaxin 6 and trans Golgi network 38 proteins, before recycling. Because we show that the exit rate from the trans Golgi apparatus back to the plasma membrane (hours) is slower than the entry rate (minutes), the neuronal postendocytic trafficking of sst2 receptor is likely to have functional consequences in several neurological diseases in which an increase in somatostatin release occurs.

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Section: Methodsmentioning

confidence: 99%

Activated Somatostatin Type 2 Receptors Traffic In Vivo in Central Neurons from Dendrites to the Trans Golgi Before Recycling

Lelouvier

Ghouzzi

Toyama

et al. 2007

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“…DNA and RNA were determined as described previously (Ch'ih et al, 1977a). Radioactivities were determined as described previously (Ch'ih et al, 1977b).…”

Section: Methodsmentioning

confidence: 99%

“…The relative turnover rate of the nuclear RNP 60min after cycloheximide administration was measured by using a dual-labelling technique similar to the procedure described by Ch'ih et al (1977b).…”

Section: Isolation Ofsubnuclear Componentsmentioning

confidence: 99%

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Regulation of mammalian protein synthesis in vivo. Simulated transport of nuclear ribonucleoprotein complexes to the cytoplasm after cycloheximide treatment

Ch'ih¹,

Duhl²,

Faulkner³

et al. 1979

Biochemical Journal

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By studies in vivo with purified nuclei from rat liver, it was shown that a non-lethal dose of cycloheximide causes a decrease in the content of total nuclear ribonucleoprotein complexes by 2h after treatment. Analysis of the complex by sucrose-density-gradient centrifugation substantiated this observation for the faster-sedimenting complex, but showed an increase in the content of a smaller complex. Radioisotope incorporation studies showed that the overall decrease in nuclear ribonucleoprotein content was not due to a decreased synthesis, but rather to an increased transport to the cytoplasm. The results of a double-radioisotope technique support the conclusion that, during the inhibitory phase of protein synthesis brough on by cycloheximdie, gene transcription continues and the gene product is transported to the cytoplasm for subsequent translation.

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“…Samples containing radioactivity were determined as described by Ch'ih et al (23). Protein was determined by the method of Lowry et al (24).…”

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confidence: 99%

Synthesis of Rat Liver Mitochondrial Proteins after the Administration of a Nonlethal Dose of Cycloheximide

Ch'ih

Froman

Devlin

1978

Experimental Biology and Medicine

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Regulation of mammalian protein synthesis in vivo. Stimulated liver ribonucleic acid synthesis in vivo after cycloheximide treatment

Cited by 24 publications

References 31 publications

Activated Somatostatin Type 2 Receptors Traffic In Vivo in Central Neurons from Dendrites to the Trans Golgi Before Recycling

Activated Somatostatin Type 2 Receptors Traffic In Vivo in Central Neurons from Dendrites to the Trans Golgi Before Recycling

Regulation of mammalian protein synthesis in vivo. Simulated transport of nuclear ribonucleoprotein complexes to the cytoplasm after cycloheximide treatment

Synthesis of Rat Liver Mitochondrial Proteins after the Administration of a Nonlethal Dose of Cycloheximide

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