Regulation of macrophage polarization and promotion of endothelialization by NO generating and PEG-YIGSR modified vascular graft

Tang, Di; Chen, Siyuan; Hou, Ding; Gao, Jingchen; Li, Jiang; Shi, Jie; Liang, Qinge; Kong, Deling; Wang, Shufang

doi:10.1016/j.msec.2017.11.005

Cited by 50 publications

(41 citation statements)

References 44 publications

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“…For immunostaining, the sections were first incubated in 1% bovine serum albumin for 30 min to block nonspecific binding. Then the sections were incubated with primary antibodies: rabbit antihuman VEGF (1:100, Servicebio, Wuhan, China) or rabbit antihuman Von Willebrand Factor (VWF: Endothelial cell marker, 1:100, Servicebio) . Bound primary antibody was visualized using an horseradish peroxidase (HRP)‐labeled goat antirabbit IgG (H + L) secondary antibody (1:200) and diaminobenzidine (DAB) kit (Servicebio).…”

Section: Methodsmentioning

confidence: 99%

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Lei

Deng

Tang

et al. 2019

Macromolecular Bioscience

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Bioprosthetic heart valves (BHVs) used in the clinic are mostly fixed by glutaraldehyde and the lack of endothelialization is a major problem for glutaraldehyde‐fixed pericardia. Hyaluronic acid is a major glycosaminoglycan that exists in native heart valves. Coupled with its inherent biocompatibility, it may enhance endothelial adhesion and proliferation when associated with vascular endothelial growth factor (VEGF). In this study, an optimized system is developed to improve the endothelialization of glutaraldehyde‐fixed pericardium. A hybrid pericardium with VEGF‐loaded hyaluronic acid hydrogel coating is developed by the crosslinking of 1,4‐butanediol diglycidyl ether. The adhesion and growth potential of human umbilical vein endothelial cells (HUVECs) on pericardia, platelet adhesion, and calcification by an in vivo rat subdermal implantation model are investigated. The results show improved HUVEC adhesion and proliferation, less platelet adhesion, and less calcification for hybrid pericardium by introducing the coating of VEGF‐loaded hyaluronic acid hydrogel. Thus, the coating of VEGF‐loaded hyaluronic acid hydrogel on pericardium is a promising approach to obtain bioprosthetic valves for clinical applications with increased endothelialization and antithrombotic and anticalcification properties.

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Section: Methodsmentioning

confidence: 99%

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Lei

Deng

Tang

et al. 2019

Macromolecular Bioscience

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“…However, it lacks cellrecognition sites and tends to attract platelets and plasma proteins. 16 PEG with super-hydrophilic chains prevents protein adsorption via its steric barrier 20…”

Section: Final Aimmentioning

confidence: 99%

Coaxial PCL/PEG-thiol–ene microfiber with tunable physico-chemical properties for regenerative scaffolds

et al. 2019

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“…in the untreated. (100) Other authors have attached cell-adhesive peptides (101–105) or added cell-friendly biochemicals (102,103,105–107) to entice engraftment.…”

Section: Designs and Progressmentioning

confidence: 99%

Quickening: Translational design of resorbable synthetic vascular grafts

Stowell

Wang

2018

Biomaterials

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Traditional tissue-engineered vascular grafts have yet to gain wide clinical use. The difficulty of scaling production of these cell- or biologic-based products has hindered commercialization. In situ tissue engineering bypasses such logistical challenges by using acellular resorbable scaffolds. Upon implant, the scaffolds become remodeled by host cells. This review describes the scientific and translational advantages of acellular, synthetic vascular grafts. It surveys in vivo results obtained with acellular synthetics over their fifty years of technological development. Finally, it discusses emerging principles, highlights strategic considerations for designers, and identifies questions needing additional research.

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Regulation of macrophage polarization and promotion of endothelialization by NO generating and PEG-YIGSR modified vascular graft

Cited by 50 publications

References 44 publications

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Coaxial PCL/PEG-thiol–ene microfiber with tunable physico-chemical properties for regenerative scaffolds

Quickening: Translational design of resorbable synthetic vascular grafts

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