Regulation of macrophage inflammatory protein-2 gene expression in response to 2,4-dinitrofluorobenzene in RAW 264.7 cells

Effects of nanoparticulate saponin-platinum conjugates on 2,4-dinitrofluorobenzene-induced macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells

“…In addition, the exposure of skin-derived dendritic cells to DNFB induces activation of the p38 MAP kinase and ERK1/2 in the regulation of CD40 expression (5). In a previous study, we found that the production of ROS was associated with activation of the MIP-2 gene promoter by DNFB, and that this occurred via activation of the p38 MAP kinase and ERK1/2 pathways (16,17).…”

Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells

“…However, considering the variation observed during experiments in the THP-1 human monocytic leukemia cell line as a model system, the effect of skin sensitizers on CD83 expression appears to be unstable (25). We reported previously that DNFB is involved in activating mouse macrophages in the dermis of NC/Nga mice (19). The results showed that DNFB upregulates MIP-2 expression in the dermis and the RAW264.7 macrophage cell line based on the MAPK signaling pathway.…”

“…To analyze CD83 expression, total RNAs were extracted with an RNeasy Mini Kit (Qiagen, Valencia, CA, USA) from RAW 264.7 cells or skin tissues, and cDNA was generated as described previously (19). One microliter of the cDNA mixture was subjected to the standard PCR reaction for 25 cycles of denaturation for 30 sec at 95ºC, annealing for 60 sec at 58ºC , and elongation for 60 sec at 72 o C. The primer sequences used are as followings.…”

“…However, exposure of DCs to nickel sulfate stimulates signal transduction pathways and augments expression of MHC class II and several costimulatory molecules in different manners compared to that of DNFB (16). Macrophages are a subpopulation of cells in the mouse dermis that have gained attention as a useful therapeutic target for contact allergies (17)(18)(19). In a previous study, we examined macrophage inflammatory protein 2 (MIP-2) expression in topically DNFB-applied skin and the DNFB-treated RAW 264.7 cells (19).…”

“…Macrophages are a subpopulation of cells in the mouse dermis that have gained attention as a useful therapeutic target for contact allergies (17)(18)(19). In a previous study, we examined macrophage inflammatory protein 2 (MIP-2) expression in topically DNFB-applied skin and the DNFB-treated RAW 264.7 cells (19). We found that the production of ROS is associated with MIP-2 gene expression induced by DNFB via activation of p38 MAP kinase and ERK1/2 in macrophages (20,21).…”