Regulation of macrophage activation

Ma, Jin; Chen, T.; Mandelin, Jami; Ceponis, A; Miller, Nicole E.; Hukkanen, Mika; F., G.; Konttinen, Yrjö T.

doi:10.1007/s00018-003-3020-0

Cited by 300 publications

(248 citation statements)

References 79 publications

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“…In the current study the authors observed that the different doses of CMF increased expression of IL-2 and IFN-γ (Th1 cytokines). Th1 cytokines are implicated in the development of the cellular immune response and macrophage activation (Williams et al, 1995;Ma et al, 2003). The authors therefore postulated that the increase of IL-2 and IFN-γ induced by the CMF supplement may increase the cellular immune response.…”

Section: Discussionmentioning

confidence: 99%

Fermentation products of <i>Cordyceps militaris</i> enhance performance and modulate immune response of weaned piglets

Cheng¹,

Wen²,

Dybus³

et al. 2016

SA J. An. Sci.

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The aim of this study was to investigate the effect of supplementation of Cordyceps militaris fermentation products (CMF) on growth performance and immunocompetence of piglets. The study involved three groups of animals, which were supplemented with CMF (500, 1000 and 1500 μg/kg feed), and a control group. CMF supplementation significantly increased growth performance in weaned piglets. Bodyweight gain, average daily gain and feed intake in animals supplemented with 1000 µg CMF/kg feed were significantly higher in comparison with the control group. In addition, CMF supplementation only significantly increased the synthesis of Th1 cytokines, as indicated by the levels of IL-2 and IFN-γ. The piglets fed with the CMF supplement displayed an increased cellular immune response. Indeed, alveolar macrophages isolated from piglets supplemented with 1000 and 1500 µg CMF/kg feed had significantly higher chemotactic and phagocytic indices than those isolated from piglets that received the control feed or feed supplemented with 500 µg CMF/kg. In relation to the absence of effect on Th2 cytokines, the CMF supplement had no effect on hog cholera antibody titre. In summary, feed supplementation with CMF improves growth performance and enhances cell-mediated immunity. CMF supplementation may thus be useful at weaning to counteract physiological and immunological stress during this period. ______________________________________________________________________________________

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Section: Discussionmentioning

confidence: 99%

Fermentation products of <i>Cordyceps militaris</i> enhance performance and modulate immune response of weaned piglets

Cheng¹,

Wen²,

Dybus³

et al. 2016

SA J. An. Sci.

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“…Cytokine environment and cell-cell interaction are important factors in the adaptive immune response. As professional antigen-presenting cells, macrophages not only respond to Th1 and Th2 cytokines and alter their functional phenotypes, but also secrete different amounts of M1 and M2 cytokines and express antigen uptake and presenting related surface markers (such as mannose receptor, MHC and costimulatory molecules) to present antigen protein and activate T/B cells [13,33] . The effects of andrographolide were thus investigated on the antigen-uptake and presenting capacity related suface markers of macrophages in vitro and specific antibody response in vivo.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory activity of andrographolide on macrophage activation and specific antibody response

et al. 2010

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Aim: To investigate the immunomodulatory effects of andrographolide on both innate and adaptive immune responses. Methods: Andrographolide (10 µg/mL in vitro or 1 mg/kg in vivo) was used to modulate LPS-induced classical activated (M1) or IL-4-induced alternative activated (M2) macrophages in vitro and humor immune response to HBsAg in vivo. Cytokine gene expression profile (M1 vs M2) was measured by real-time PCR, IL-12/IL-10 level was detected by ELISA, and surface antigen expression was evaluated by flow cytometry, whereas phosphorylation level of ERK 1/2 and AKT was determined by Western blot. The level of anti-HBs antibodies in HBsAg immunized mice was detected by ELISA, and the number of HBsAg specific IL-4-producing splenocyte was enumerated by ELISPOT. Results: Andrographolide treatment in vitro attenuated either LPS or IL-4 induced macrophage activation, inhibited both M1 and M2 cytokines expression and decreased IL-12/IL-10 ratio (the ratio of M1/M2 polarization). Andrographolide down-regulated the expression of mannose receptor (CD206) in IL-4 induced macrophages and major histocompability complex/costimulatory molecules (MHC I, CD40, CD80, CD86) in LPS-induced macrophages. Correspondingly, anti-HBs antibody production and the number of IL-4-producing splenocytes were reduced by in vivo administration of andrographolide. Reduced phosphorylation levels of ERK1/2 and AKT were observed in macrophages treated with andrographolide. Conclusion: Andrographolide can modulate the innate and adaptive immune responses by regulating macrophage phenotypic polarization and Ag-specific antibody production. MAPK and PI3K signaling pathways may participate in the mechanisms of andrographolide regulating macrophage activation and polarization.

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“…T cells or T cell-derived cytokines are also able to regulate Fc␥R expression on macrophages either directly (29) or indirectly by producing cytokines like IFN␥ (30). This can explain why during a T cell-mediated arthritis such as antigen-induced arthritis (AIA), joint inflammation has been shown to follow an Fc␥RI-dependent pathway (12), whereas Fc␥RIII dependency is completely lost.…”

Section: Discussionmentioning

confidence: 99%

Joint inflammation and chondrocyte death become independent of Fcγ receptor type III by local overexpression of interferon‐γ during immune complex–mediated arthritis

Nabbe¹,

Boross²,

Holthuysen³

et al. 2005

Arthritis & Rheumatism

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Objective. It has previously been shown that the onset and the degree of joint inflammation during immune complex (IC)-mediated arthritis depend on Fc␥ receptor type III (Fc␥RIII). Local adenoviral overexpression of interferon-␥ (IFN␥) in the knee joint prior to onset of IC-mediated arthritis aggravated severe cartilage destruction. In Fc␥RI Ϫ/Ϫ mice, however, chondrocyte death was not enhanced by IFN␥, whereas matrix metalloproteinase (MMP)-mediated aggrecan breakdown was markedly elevated, suggesting a role for the activating Fc␥RIII in the latter process. We undertook this study to determine the role of Fc␥RIII in joint inflammation and severe cartilage destruction in IFN␥-stimulated IC-mediated arthritis, using Fc␥RIII ؊/؊ mice.Methods. Fc␥RIII ؊/؊ and wild-type (WT) mice were injected in the knee joint with recombinant adenovirus encoding murine IFN␥ (AdIFN␥) or with adenovirus encoding enhanced green fluorescent protein 1 day prior to induction of IC-mediated arthritis. Histologic sections were obtained 3 days after arthritis onset to study inflammation and cartilage damage. MMPmediated expression of the VDIPEN neoepitope was detected by immunolocalization. Chemokine and Fc␥R expression levels were determined in synovial washouts and synovium, respectively.Results. Injection of AdIFN␥ in naive knee joints markedly increased levels of messenger RNA for Fc␥RI, Fc␥RII, and Fc␥RIII. Upon IFN␥ overexpression prior to induction of IC-mediated arthritis, joint inflammation was similar in Fc␥RIII ؊/؊ and WT mice. The percentage of macrophages in the knee joint was increased, which correlated with high concentrations of the macrophage attractant macrophage inflammatory protein 1␣. Furthermore, IFN␥ induced 2-fold and 3-fold increases in chondrocyte death in WT controls and Fc␥RIII ؊/؊ mice, respectively. Notably, VDIPEN expression also remained high in Fc␥RIII ؊/؊ mice.Conclusion. IFN␥ bypasses the dependence on Fc␥RIII in the development of IC-mediated arthritis. Furthermore, both Fc␥RI and Fc␥RIII can mediate MMP-dependent cartilage matrix destruction.

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Regulation of macrophage activation

Cited by 300 publications

References 79 publications

Fermentation products of <i>Cordyceps militaris</i> enhance performance and modulate immune response of weaned piglets

Fermentation products of <i>Cordyceps militaris</i> enhance performance and modulate immune response of weaned piglets

Immunomodulatory activity of andrographolide on macrophage activation and specific antibody response

Joint inflammation and chondrocyte death become independent of Fcγ receptor type III by local overexpression of interferon‐γ during immune complex–mediated arthritis

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