Regulation of long-chain polyunsaturated fatty acid biosynthesis in teleost fish

Xie, Dizhi; Chen, Cuiying; Dong, Yewei; You, Cuihong; Wang, Shuqi; Monroig, Óscar; Tocher, Douglas R.; Li, Yuanyou

doi:10.1016/j.plipres.2021.101095

Cited by 89 publications

(53 citation statements)

References 167 publications

(363 reference statements)

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“…Other molecules, with p values ranging between 10E−12 and 0.02, comprise upregulated lipids. The annotated lipid with lower p value was potentially a PE 42:10, that is, a long chain PE with a very high unsaturation, which might be derived from the elongation steps of docosahexaenoic‐containing lipids (Xie et al., 2021). Similarly, we found a dimethyl‐PE (PE‐NMe2(32:1) which is formed by sequential methylation of phosphatidylethanolamine as part of a mechanism for biosynthesis of phosphatidylcholine (Blusztajn et al., 1979).…”

Section: Resultsmentioning

confidence: 99%

Nuclear lipidome is altered in amyotrophic lateral sclerosis: A pilot study

Ramírez‐Núñez

Jové

Torres

et al. 2021

Journal of Neurochemistry

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Nucleocytosolic transport, a membrane process, is impaired in motor neurons in amyotrophic lateral sclerosis (ALS). This study analyzes the nuclear lipidome in motor neurons in ALS and examines molecular pathways linked to the major lipid alterations. Nuclei were obtained from the frozen anterior horn of the lumbar spinal cord of ALS patients and age-matched controls. Lipidomic profiles of this subcellular fraction were obtained using liquid chromatography and mass spectrometry. We validated the mechanisms behind presumable lipidomic changes by exploring ALS surrogate models including human motor neurons (derived from ALS lines and controls) subjected to oxidative stress, the hSOD-G93A transgenic mice, and samples from an independent cohort of ALS patients. Among the differential lipid species, we noted 41 potential identities, mostly belonging to phospholipids (particularly ether phospholipids, as plasmalogens), as well as diacylglycerols and triacylglycerides. Decreased expression of alkyldihydroxyacetonephosphate synthase (AGPS)-a critical peroxisomal enzyme in plasmalogen synthesis-is found in motor neuron disease models; this occurs in parallel with an increase in the expression of sterol carrier protein 2 (SCP2) mRNA in ALS and Scp2 levels in G93A transgenic mice. Further, we identified diminished expression of diacylglycerol-related enzymes, such as phospholipase C βI | 483 RAMÍREZ-NUÑEZ Et Al.

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Section: Resultsmentioning

confidence: 99%

Nuclear lipidome is altered in amyotrophic lateral sclerosis: A pilot study

Ramírez‐Núñez

Jové

Torres

et al. 2021

Journal of Neurochemistry

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“…Within this context, S. senegalensis has been shown to have a higher LC-PUFA biosynthesising capacity compared to most of its marine counterparts [ 21 , 30 ]. In the present study, we investigated the combined effect of diet and salinity, the latter being regarded as an environmental factor through which LC-PUFA biosynthesis can be modulated in telosts [ 18 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, Elovl are enzymes that catalyse the first and rate-limiting condensation reaction in the fatty acid (FA) elongation pathway [ 16 ]. There exist eight different Elovl present in vertebrates (Elovl1-8) [ 18 ], among which Elovl5 and Elovl2 have well-established roles in LC-PUFA biosynthesis since they are highly efficient in the elongation of PUFA substrates with chain lengths varying from 18 to 22 carbons [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition to diet, environmental factors, such as salinity, can also modulate the activity of the LC-PUFA biosynthetic pathways in fish [ 31 , 32 , 33 ]. While the specific mechanisms involved are not yet fully understood [ 18 ], the modification of ambient salinity arises as a potential cost-effective strategy to enhance the LC-PUFA biosynthetic pathways in species such as S. senegalensis that are capable of acclimatising to different osmotic conditions [ 34 , 35 ]; this can be easily set up in recirculation aquaculture systems (RAS) [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis

et al. 2021

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Fish vary in their ability to biosynthesise long-chain polyunsaturated fatty acids (LC-PUFA) depending upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. It has been reported in Solea senegalensis the existence of a Δ4 desaturase, enabling the biosynthesis of docosahexaenoic acid (DHA) from eicosapentaenoic acid (EPA), which can be modulated by the diet. The present study aims to evaluate the combined effects of the partial replacement of fish oil (FO) with vegetable oils and reduced environmental salinity in the fatty acid composition of relevant body compartments (muscle, hepatocytes and enterocytes), the enzymatic activity over α-linolenic acid (ALA) to form n-3 LC-PUFA through the incubation of isolated hepatocytes and enterocytes with [1-14C] 18:3 n-3, and the regulation of the S. senegalensis fads2 and elovl5 in the liver and intestine. The presence of radiolabelled products, including 18:4n-3, 20:4n-3 and EPA, provided compelling evidence that a complete pathway enabling the biosynthesis of EPA from ALA, establishing S. senegalensis, has at least one Fads2 with ∆6 activity. Dietary composition prevailed over salinity in regulating the expression of fads2, while salinity did so over dietary composition for elovl5. FO replacement enhanced the proportion of DHA in S. senegalensis muscle and the combination with 20 ppt salinity increased the amount of n-3 LC-PUFA in hepatocytes.

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“…Almost 60% of the glucose taken up by HCC cells is used for the synthesis of fatty acids (17)(18)(19). Sterol regulatory element binding protein (SREBP)-1 is the most important transcription factor in lipid metabolism (20)(21)(22). In HCC cells, SREBP-1 mediates the transcription of genes related to the syntheses of fatty acids and triglycerides (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of SREBP-1 Activation by a Novel Small-Molecule Inhibitor Enhances the Sensitivity of Hepatocellular Carcinoma Tissue to Radiofrequency Ablation

2021

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Radiofrequency ablation (RFA) is an important strategy for treatment of advanced hepatocellular carcinoma (HCC). However, the prognostic indicators of RFA therapy are not known, and there are few strategies for RFA sensitization. The transcription factor sterol regulatory element binding protein 1 (SREBP)-1 regulates fatty-acid synthesis but also promotes the proliferation or metastasis of HCC cells. Here, the clinical importance of SREBP-1 and potential application of knockdown of SREBP-1 expression in RFA of advanced HCC was elucidated. In patients with advanced HCC receiving RFA, a high level of endogenous SREBP-1 expression correlated to poor survival. Inhibition of SREBP-1 activation using a novel small-molecule inhibitor, SI-1, not only inhibited the aerobic glycolysis of HCC cells, it also enhanced the antitumor effects of RFA on xenograft tumors. Overall, our results: (i) revealed the correlation between SREBP-1 and HCC severity; (ii) indicated that inhibition of SREBP-1 activation could be a promising approach for treatment of advanced HCC.

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Regulation of long-chain polyunsaturated fatty acid biosynthesis in teleost fish

Cited by 89 publications

References 167 publications

Nuclear lipidome is altered in amyotrophic lateral sclerosis: A pilot study

Nuclear lipidome is altered in amyotrophic lateral sclerosis: A pilot study

Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis

Inhibition of SREBP-1 Activation by a Novel Small-Molecule Inhibitor Enhances the Sensitivity of Hepatocellular Carcinoma Tissue to Radiofrequency Ablation

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