Regulation of lncRNA and Its Role in Cancer Metastasis

Li, Juan; Meng, Hui; Bai, Yun; Wang, Kai

doi:10.3727/096504016x14549667334007

Cited by 231 publications

(164 citation statements)

References 123 publications

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“…Increasing reports of aberrant expression of lncRNAs across numerous cancer types suggested that some lncRNAs could promote the formation and development of tumors 16–18. With regard to these latter effects, expression levels of certain lncRNAs have been shown to be associated with the recurrence, metastasis, and prognosis of various cancers (HCC, gastric carcinoma, and breast carcinoma) 19–21.…”

Section: Discussionmentioning

confidence: 99%

High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma

Zhang¹,

Tian²,

Zhang³

et al. 2016

OTT

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PurposeEmerging studies show that long noncoding RNAs (lncRNAs) have important roles in carcinogenesis. This study investigated the role of lncRNA highly upregulated in liver cancer (HULC) expression in glioma and its clinical significance in glioma patients.Materials and methodsHULC expression was detected in glioma tissues and cell lines by using real-time quantitative reverse transcription polymerase chain reactions. Association between HULC levels and clinicopathological factors and patients prognosis was also analyzed. Expression of HULC was restored and knocked down in glioma cell line U87 by using HULC cDNA and siRNA, respectively. CCK-8 and colony formation assays were used to investigate the role of HULC in the regulation of proliferation of glioma cells.ResultsHULC was highly expressed in glioma tissues, being closely related to age and grade of glioma. Univariate survival analysis demonstrated that high HULC levels were significantly associated with overall survival (OS) (hazard ratio [HR], 0.422; 95% confidence interval [CI], 0.220–0.806; P=0.009), and it remained an independent predictor for OS (HR, 0.340; 95% CI, 0.175–0.659; P=0.001) in multivariate Cox regression analysis. Functionally, forced expression of HULC results in increased cell proliferation and colony formation of U87 glioma cell line, whereas knockdown of HULC expression reduced these oncogenic properties of glioma cells.ConclusionThese findings suggest that HULC may play an important role in glioma progression and will be further evaluated as a biomarker for predicting the survival of glioma patients.

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Section: Discussionmentioning

confidence: 99%

High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma

Zhang¹,

Tian²,

Zhang³

et al. 2016

OTT

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“…However, only a small proportion of lncRNAs have been demonstrated to be biologically functional (20). Among the functional lncRNAs, the majority have been implicated in malignant tumors (10,21). The lncRNA, EGOT, was initially thought to be involved in eosinophil development and expressed in mature eosinophils (16); it was subsequently demonstrated that EGOT locus is highly structured, containing several evolutionary conserved regions, and thermodynamic stable secondary structures (22).…”

Section: Discussionmentioning

confidence: 99%

“…With continuous advances in research approaches, research focused on lncRNAs has recently undergone a rapid expansion. LncRNAs are closely associated with tumor development (9,10) and particularly, have been implicated in glioma progression (11). For example, the lncRNA, MALAT1, has been demonstrated to have crucial roles in the cell proliferation, metastasis and apoptosis processes in glioma (12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA eosinophil granule ontogeny transcript inhibits cell proliferation and migration and promotes cell apoptosis in human glioma

Liang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Glioma is the most common primary brain tumor and represents one of the most aggressive and lethal types of human cancer. Recent advances have implicated long noncoding RNAs (lncRNAs) as crucial mediators of cancer development and progression. The present study aimed to investigate the role of a newly-discovered lncRNA, termed eosinophil granule ontogeny transcript (EGOT), in the aggressive abilities of cells in human glioma. It was initially found that the relative transcription level of EGOT in glioma cancerous tissues was significantly lower than that in adjacent non-cancerous tissues. EGOT was differentially expressed in a series of glioma cell lines, with its lowest level in high aggressive U251 and U87 cells. When EGOT was overexpressed by an expression plasmid, cell viability was significantly inhibited in U251 and U87 cells. Furthermore, with EGOT overexpression, the cell cycle was arrested at G0/G1 phase and consequently, cell apoptosis was significantly promoted along with the activities of caspase-3 and caspase-9. The migration abilities of EGOT-overexpressed cells were inhibited by 71.4% in U251 cells and by 69.5% in U87 cells. These data suggest that overexpression of EGOT inhibits cell proliferation and migration, and promotes cell apoptosis in glioma. Therefore, EGOT has potent anticancer activity and may function as a tumor suppressor in human glioma. IntroductionGlioma is the most common brain tumor, accounting for >50% of all brain tumors (1). According to a population-based investigation, the overall median survival duration for patients with malignant glioma is ~15 months (2). Despite significant advances in the therapeutic strategies of glioma, including neurosurgical approaches, chemotherapy and radiotherapy, the prognosis of patients with malignant glioma remains poor, primarily due to the fact that malignant glioma cells are highly aggressive and capable of infiltrating into adjacent normal brain tissue, leading to the eventual failure of complete surgical dissection of the tumor (3,4). Recurrence and resistance to chemotherapy are to be claimed two influential factors for prognosis (5,6). Therefore, developing novel strategies and elucidating innovative therapeutic agents for patients suffering from glioma are imperative and urgent tasks.Long noncoding RNAs (lncRNAs) are a type of RNAs that widely exist in the nuclei and cytoplasm of eukaryotic cells. These RNAs are non-protein coding and >200 nucleotides in length (7,8). With continuous advances in research approaches, research focused on lncRNAs has recently undergone a rapid expansion. LncRNAs are closely associated with tumor development (9,10) and particularly, have been implicated in glioma progression (11). For example, the lncRNA, MALAT1, has been demonstrated to have crucial roles in the cell proliferation, metastasis and apoptosis processes in glioma (12-14). MALAT1 was proposed as a tumor suppressor in glioma (14). Knockdown of MALAT1 increases the blood-tumor barrier permeability (15), and affects proliferation and ...

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“…LncRNAs have been recently reported to play an important role in various malignancies, including CRC. [18][19][20] However, global lncRNA expression profile in CRC is not fully clear, and more evidences are needed to uncover the role of lncRNAs in CRC. In this study, we performed microarray analysis in CRC tissue and paired normal tissue to investigate the global expression profile of lncRNA and look for lncRNAs that have potential to be a CRC diagnostic biomarkers.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of long non-coding RNA expression and function in colorectal cancer

et al. 2017

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Long non-coding RNAs (lncRNAs) are widely transcribed in the genome, but their expression profile and roles in colorectal cancer are not well understood. The aim of this study was to investigate the long non-coding RNA expression profile in colorectal cancer and look for potential diagnostic biomarkers of colorectal cancer. Long noncoding RNA microarray was applied to investigate the global long non-coding RNA expression profile in colorectal cancer. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed using standard enrichment computational methods. The expression levels of selected long non-coding RNAs were validated by quantitative reverse transcription polymerase chain reaction. The relationship between long non-coding RNA expression levels and clinicopathological characteristics of colorectal cancer patients was assessed. Coexpression analyses were carried out to find the coexpressed genes of differentially expressed long non-coding RNAs, followed by gene ontology analysis to predict the possible role of the selected long non-coding RNAs in colorectal carcinogenesis. In this study, a total of 1596 long non-coding RNA transcripts and 1866 messenger RNA transcripts were dysregulated in tumor tissues compared with paired normal tissues. The top upregulated long non-coding RNAs in tumor tissues were CCAT1, UCA1, RP5-881L22.5, NOS2P3, and BC005081 and the top downregulated long non-coding RNAs were AK055386, AC078941.1, RP4-800J21.3, RP11-628E19.3, and RP11-384P7.7. Long non-coding RNA UCA1 was significantly upregulated in colon cancer, and AK055386 was significantly downregulated in tumor with dimension <5 cm. Functional prediction analyses showed that both the long non-coding RNAs coexpress with cell cycle related messenger RNAs. The current long non-coding RNA study provided novel insights into expression profile in colorectal cancer and predicted the potential roles of long non-coding RNAs in colorectal carcinogenesis. Among the dysregulated long non-coding RNAs, UCA1 was found to be associated with anatomic site, and AK055386 was found associated with tumor size. Further functional investigations into the molecular mechanisms are warranted to clarify the role of long non-coding RNA in colorectal carcinogenesis.

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Regulation of lncRNA and Its Role in Cancer Metastasis

Cited by 231 publications

References 123 publications

High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma

High expression of long noncoding RNA HULC is a poor predictor of prognosis and regulates cell proliferation in glioma

Long noncoding RNA eosinophil granule ontogeny transcript inhibits cell proliferation and migration and promotes cell apoptosis in human glioma

Genome-wide analysis of long non-coding RNA expression and function in colorectal cancer

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