Regulation of leukemic cell adhesion, proliferation, and survival by β-catenin

Chung, Eun Joo; Hwang, Sang‐Gu; Nguyen, Phuongmai; Lee, Sunmin; Kim, Jung-Sik; Kim, Jin Woo; Henkart, Pierre A.; Bottaro, Donald P.; Soon, Lilian; Bonvini, Paolo; Lee, Su Jae; Karp, Judith E.; Oh, Ho Jung; Rubin, Jeffrey S.; Trepel, Jane B.

doi:10.1182/blood.v100.3.982

Cited by 119 publications

(126 citation statements)

References 57 publications

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“…This is not only time-and reagent-consuming, but the transfection efficiency also varies from experiment to experiment. To solve these problems, a stable clone needs to be established by co-transfecting reporter-and antibiotic-containing plasmids (20,22,23). In the present study, we successfully established stable clone HEK 293-TOP cells that contained a bi-functional reporter plasmid.…”

Section: Discussionmentioning

confidence: 94%

“…It is well known that the N-terminus of β-catenin (Ser33/Ser37/Thr41) is phosphorylated by GSK-3β and then targeted for ubiquitin-dependent degradation. Although mutation at the N-terminus of β-catenin has been found in some tumors, mutations at these sites have not been found in Jurkat cells (20). NCTD has been reported to inhibit protein phosphatase 2A (PP2A) activity and PP2A has been found to positively control Wnt/β-catenin signaling upon Wnt stimulation (31,32).…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that Jurkat cells express high levels of Wnt/β-catenin signaling and play an important role in Jurkat cell proliferation (20). To examine whether activation of Wnt/β-catenin signaling in a leukemic cancer cell line was blocked by NCTD, we established the Jurkat-TOP and Jurkat-FOP stable cells by pGL4-TOP and pGL4-FOP transfection, respectively.…”

Section: Luciferase Activity Of Jurkat-top Cells Was Activated By Wntmentioning

confidence: 99%

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Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system

Chuang

Lieu

Tsai

et al. 2010

Braz J Med Biol Res

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Luciferase Activity Of Jurkat-top Cells Was Activated By Wntmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system

Chuang

Lieu

Tsai

et al. 2010

Braz J Med Biol Res

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“…The most frequent leukemia in the child is acute lymphoblastic leukemia (ALL). Several papers concerning this subject indicate an activation of the Wnt signaling pathway in ALL [Khan et al, 2007] and an increase in the concentration of β-catenin [Chung et al, 2002]. Other articles showed inhibition of the Wnt signaling pathway as "an attractive target for the use of more specific therapies…" [Román-Gómez, 2007].…”

Section: Discussionmentioning

confidence: 99%

In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells

Collard

Mertens

Hinsenkamp

2010

Bioelectromagnetics

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An acceleration of differentiation, at the expense of proliferation, is observed after exposure of various biological models to low frequency and low amplitude electric and electromagnetic fields. Following these results showing significant modifications, we try to identify the biological mechanism involved at the cell level through microarray screening. For this study, we use epidermis cultures harvested from human abdominoplasty. Two platinum electrodes are used to apply the electric signal. The gene expressions of 38,500 well-characterized human genes are analyzed using Affymetrix® microarray U133 Plus 2.0 chips. The protocol is repeated on three different patients. After three periods of exposure, a total of 24 chips have been processed. After the application of ELF electric fields, the microarray analysis confirms a modification of the gene expression of epidermis cells. Particularly, four up-regulated genes (DKK1, TXNRD1, ATF3, and MME) and one down-regulated gene (MACF1) are involved in the regulation of proliferation and differentiation. Expression of these five genes was also confirmed by real-time rtPCR in all samples used for microarray analysis. These results corroborate an acceleration of cell differentiation at the expense of cell proliferation.

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“…Wnt/b-catenin has previously been implicated in the development of human leukaemia: McWhirter et al (1999) showed that the E2a-Pbx fusion protein found in precursor B-acute lymphoblastic leukaemia (ALL) activates the expression of Wnt-16 resulting in a potential autocrine signalling loop. Chung et al (2002) showed that b-catenin protein was expressed in leukaemia cell lines and primary cells to varying levels. Inhibition of the Wnt/b-catenin pathway in Jurkat T-lymphoblastic cells impaired proliferation and increased susceptibility to apoptosis in response to One of the defining features of AML is impaired differentiation of the malignant cells to mature granulocytes and monocytes.…”

Section: Cd34+ Patient Number --------------Aml Cases--------------mentioning

confidence: 99%

Constitutive activation of the Wnt/β-catenin signalling pathway in acute myeloid leukaemia

et al. 2005

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The b-catenin protein is at the core of the canonical Wnt signalling pathway. Wnt stimulation leads to b-catenin accumulation, nuclear translocation and interaction with T-cell factor/lymphoid enhancer factor (TCF/LEF) transcription factors to regulate genes important for embryonic development and proliferation. Wnt/b-catenin can promote stem cell self-renewal and is dysregulated in colon carcinoma. We have examined the role of the Wnt pathway in the development of acute myeloid leukaemia (AML) and find that the b-catenin protein is readily detected in primary AML samples. Using transfection of a TCF/LEF reporter construct into primary AML cells and normal human progenitors, we find increased reporter activity in 16/25 leukaemia samples. Retrovirally mediated expression of a mutant active b-catenin in normal progenitors preserves CD34 expression and impairs myelomonocytic differentiation. Activation of TCF/LEF signalling decreases factor withdrawal-induced apoptosis of normal progenitors. A significant proportion of AML cases show aberrant expression of components of the Wnt pathway including Wnt-1, Wnt-2b and LEF-1. These results provide evidence for the involvement of the Wnt/b-catenin pathway in the pathogenesis of AML.

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Regulation of leukemic cell adhesion, proliferation, and survival by β-catenin

Cited by 119 publications

References 57 publications

Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system

Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system

In vitro study of the effects of ELF electric fields on gene expression in human epidermal cells

Constitutive activation of the Wnt/β-catenin signalling pathway in acute myeloid leukaemia

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