Regulation of Isoflavonoid Biosynthesis in Soybean Seeds

Dhaubhadel, Sangeeta

doi:10.5772/15120

Cited by 12 publications

(10 citation statements)

References 96 publications

(92 reference statements)

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“…Both compounds decreased significantly during the initial stage (30 min) of dehydration. Genistin, a 7-0 glycoside form of the aglycone genistein [34], is involved in the isoflavonoid pathway and significantly accumulates in the roots during the course of water stress. The same effect had been reported in soybean seeds where drought increased genistin content in seeds by 47% [35].…”

Section: Resultsmentioning

confidence: 99%

Comparative Metabolome Profile between Tobacco and Soybean Grown under Water-Stressed Conditions

Rabara¹,

Tripathi

Rushton³

2017

BioMed Research International

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Understanding how plants respond to water deficit is important in order to develop crops tolerant to drought. In this study, we compare two large metabolomics datasets where we employed a nontargeted metabolomics approach to elucidate metabolic pathways perturbed by progressive dehydration in tobacco and soybean plants. The two datasets were created using the same strategy to create water deficit conditions and an identical metabolomics pipeline. Comparisons between the two datasets therefore reveal common responses between the two species, responses specific to one of the species, responses that occur in both root and leaf tissues, and responses that are specific to one tissue. Stomatal closure is the immediate response of the plant and this did not coincide with accumulation of abscisic acid. A total of 116 and 140 metabolites were observed in tobacco leaves and roots, respectively, while 241 and 207 were observed in soybean leaves and roots, respectively. Accumulation of metabolites is significantly correlated with the extent of dehydration in both species. Among the metabolites that show increases that are restricted to just one plant, 4-hydroxy-2-oxoglutaric acid (KHG) in tobacco roots and coumestrol in soybean roots show the highest tissue-specific accumulation. The comparisons of these two large nontargeted metabolomics datasets provide novel information and suggest that KHG will be a useful marker for drought stress for some members of Solanaceae and coumestrol for some legume species.

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Section: Resultsmentioning

confidence: 99%

Comparative Metabolome Profile between Tobacco and Soybean Grown under Water-Stressed Conditions

Rabara¹,

Tripathi

Rushton³

2017

BioMed Research International

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“…Plants were grown in the area of Tanjungsari, Sumedang (864 m above sea level) in February 2019. Pods were harvested when the plant has reached the R5 phase because isoflavone accu mulation in soybeans occurs during the seed maturation stage (Dhaubhadel 2011). Pods were collected, cleaned, and drained, wrapped using aluminum foil with the label, frozen in liquid nitrogen, and stored in −80°C.…”

Section: Plant and Seed Samplesmentioning

confidence: 99%

“…Then, cinnamic acid is produced through the Phenylalanine AmmoniaLyase (PAL) enzyme activity. In the subsequent phase reaction, cinnamate 4hydroxylase and 4coumarate CoA ligase convert cinnamic acid into pcoumaroyl CoA (Dhaubhadel 2011). Previous studies showed that CHR, CHI1A, and IFS2 are genes that play an important role in isoflavone biosynthesis (Yu et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Expression profiling of the CHS8, CHI1A, IFS2, and CHR genes in black soybean seed [Glycine max (L). Merr.] of F4 generation

Sumardi

Mumtaza

Maulani

et al. 2020

Indones. J. Biotechnol.

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Black soybean [Glycine max (L.) Merr.] produces isoflavones as secondary metabolites, which have many benefits for human health and plant defense system. Expression profiling can guide potential work in functional genomics of the isoflavone biosynthesis pathway. Previous studies showed the vital role of the CHS8, CHI1A, and IFS2 genes in isoflavone biosynthesis. However, expression profiling of these genes in the local black soybean varieties is still limited. This study investigated the gene expression levels of the CHS8, CHI1A, IFS2, and CHR genes in local varieties, namely, UP106 (high isoflavone) and UP122 (low isoflavone) and its progenies, i.e., UP106xUP122 and UP122xUP106. Relative gene expression profiling was conducted on the basis of Reverse Transcriptase Polymerase Chain Reaction (RT‐PCR) with ACT2/7 as a housekeeping gene. As a result, the expression level of CHS8 in UP122 is lower than that in UP106. No significant difference in the expression level of CHI1A was observed in all samples. The expression levels of CHS8 and CHI1A in both progenies were higher than that in the parental line, whereas the expression levels of IFS2 in both progenies were lower than that in the parental line. CHS8 and IFS2 expression from UP106xUP122 was higher than that from UP122xUP106, whereas CHI1A expression from UP122xUP106 was higher than that from UP106xUP122. CHR showed a high expression in the reciprocal cross; however, this expression did not exceed from UP106. In conclusion, the crossing between parental lines did not affect the gene expression level in the isoflavone biosynthesis pathway.

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“…On the other hand, the health benefits of isoflavones prompted strategies to induce the synthesis of these compounds by non-legume plants (broccoli and tomatoes). This approach could be achieved in plants with an active phenylpropanoid pathway [9]. In fact, the flavanone naringenin is not only the substrate of isoflavone synthase, but as well that of flavanone-3-hydroxylase, involved in the biosynthesis of flavonols, anthocyanins and condensed tannins.…”

Section: Biosynthesis and Regulationmentioning

confidence: 99%

New Insights Regarding the Potential Health Benefits of Isoflavones

Danciu¹,

Suciu²,

Antal³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

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Isoflavones are a class of plant secondary metabolites, with an estrogen-like structure presenting a plethora of biological activities. The chapter discusses important facts about this class of phytoestrogens, from biosynthesis to the latest research about their health benefits. The following major points discussed are: biosynthesis, regulation, isolation, metabolism and bioavailability, isoflavones in diet and intake, and new insights regarding the therapeutic effect including cancer chemoprevention. The chapter ends with a mini review of own research of the anti-inflammatory and chemopreventive activity of isoflavonoid genistein alone and incorporated in modern pharmaceutical formulations. The chapter updates the interested researchers in the field with the latest progress regarding potential health benefits of isoflavones.

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Regulation of Isoflavonoid Biosynthesis in Soybean Seeds

Cited by 12 publications

References 96 publications

Comparative Metabolome Profile between Tobacco and Soybean Grown under Water-Stressed Conditions

Comparative Metabolome Profile between Tobacco and Soybean Grown under Water-Stressed Conditions

Expression profiling of the CHS8, CHI1A, IFS2, and CHR genes in black soybean seed [Glycine max (L). Merr.] of F4 generation

New Insights Regarding the Potential Health Benefits of Isoflavones

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