Regulation of intestinal apo A-IV mRNA abundance in rat pups during fasting and refeeding

Sato, Masao; Imaizumi, Kazunori; Mori, Hikari; Sugano, Michihiro

doi:10.1016/0005-2760(92)90080-f

Cited by 19 publications

(11 citation statements)

References 33 publications

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“…Total RNA was extracted from 1 g of liver and intestinal mucosa, using guanidium thiocyanate/cecium chloride ultracentrifugation as described by Sato et al ( 12 ).…”

Section: Methodsmentioning

confidence: 99%

Dietary Soy Protein Isolate and Its Undigested High Molecular Fraction Upregulate Hepatic ATP-Binding Cassette Transporter G5 and ATP-Binding Cassette Transporter G8 mRNA and Increase Biliary Secretion of Cholesterol in Rats

Ikeda¹,

Kudo²,

Hamada³

et al. 2009

J Nutr Sci Vitaminol

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Summary Dietary soy protein isolate (SPI) and its undigested high molecular fraction (HMF) exhaustively digested with proteases, compared with casein (CAS), significantly reduced serum and liver cholesterol concentration in rats. Biliary excretion of cholesterol was significantly higher in rats fed SPI and HMF than in those fed CAS. Hepatic expression of ATP-binding cassette transporter G5 (ABCG5) and ATP-binding cassette transporter G8 (ABCG8) mRNA was significantly higher in rats fed SPI and HMF than in those fed CAS. These observations suggest that increased biliary excretion of cholesterol in SPI and HMF groups is caused by the enhanced expression of Abcg5/Abcg8. Key Words ATP-binding cassette transporter G5, ATP-binding cassette transporter G8, bile, cholesterol, soy protein isolate ATP-binding cassette transporter G5 (ABCG5) and ATP-binding cassette transporter G8 (ABCG8) are mainly expressed in the liver and the intestine ( 1 ). It has been suggested that ABCG5 and ABCG8 have a function to excrete sterols from intestinal cells to the intestinal lumen and from the liver to the bile ( 1 ). Although functions of ABCG5 and ABCG8 on sterol excretion gradually become apparent, the contribution of ABCG5 and ABCG8 to cholesterol metabolism in the whole body is not fully understood and scarcely any information on regulation of ABCG5 and ABCG8 expression by dietary components has been obtained.It has been reported that dietary soy protein isolate (SPI) and its undigested high molecular fraction (HMF) lower serum cholesterol concentration in experimental animals ( 2 -4 ). One of the mechanisms of hypocholesterolemic effects of SPI and HMF is an increase in fecal bile acid excretion ( 3 , 4 ). The increased excretion of bile acids can be caused by their binding with SPI and HMF ( 3 , 4 ). Fecal excretion of neutral steroids originated from cholesterol was also increased by the feeding of SPI and HMF in rats fed a high-cholesterol diet ( 3 -5 ). It has been thought that increased excretion of cholesterol and the metabolite coprostanol is caused by reduced availability of bile acids for the bile salt micelle in the intestinal lumen and therefore by reduced solubility of cholesterol in the micelle ( 3 , 4 , 6 ). Although increased fecal excretion of cholesterol and coprostanol can be caused by increased expressions of ABCG5 and ABCG8 in the liver and the intestine, no information is available about whether SPI and HMF influence expressions and functions of ABCG5 and ABCG8 in the liver and the intestine. In the present study, we examined effects of SPI and HMF on cholesterol metabolism in relation to mRNA expressions of ABCG5 and ABCG8 in the liver and the intestine of rats.

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“…Total RNA was extracted from 1 g of liver and intestinal mucosa, using guanidium thiocyanate/cecium chloride ultracentrifugation as described by Sato et al ( 12 ).…”

Section: Methodsmentioning

confidence: 99%

Dietary Soy Protein Isolate and Its Undigested High Molecular Fraction Upregulate Hepatic ATP-Binding Cassette Transporter G5 and ATP-Binding Cassette Transporter G8 mRNA and Increase Biliary Secretion of Cholesterol in Rats

Ikeda¹,

Kudo²,

Hamada³

et al. 2009

J Nutr Sci Vitaminol

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“…According to Nunez et al (24), in preterm newborn infants, dietary nucleotides known to promote intestinal growth and maturation are likely to cause an increase in apoA-IV in plasma and elevate the activity of lecithin cholesterol acyl transferase. Sato et al (25) found in rat pups that a diet composed of casein and lactose increased intestinal apoA-IV levels, but without alterations of its plasma concentration. In turn, Stan et al (16) reported that carbohydrates, including lactose, caused a rapid rise in serum apoA-IV.…”

Section: Discussionmentioning

confidence: 99%

Analysis of apolipoprotein A-IV expression and the levels of blood plasmamain lipid fractions of calves during the first week of life before and after theadministration of milk replacer supplemented with lactose

Michałek¹,

Dratwa-Chałupnik²,

Jaszczuk³

2015

Turk J Vet Anim Sci

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The current study presents an analysis of main lipid fractions and expression of apolipoprotein A-IV (apoA-IV) of the blood plasma of calves during the first week of life, before and after administration of a milk replacer with additional quantity of lactose. The experiment was performed on 7 male Polish-Friesian calves, var. Black-and-White. Blood samples were collected before the morning feeding from the 6th to 8th day of life. In the evening on the 6th day of the experiment and in the morning on the 7th day of the experiment, monohydrate lactose in an amount of 2 g per 1 kg of body weight was added to the milk replacer. In all tested calves, after the administration of the additional quantity of lactose, there was a significant increase observed in expression of apoA-IV in the blood plasma. At the same time, no significant differences in the concentrations of triglycerides, total cholesterol, and low-density lipoprotein cholesterol were recorded, both before and after the administration of an additional dose of lactose. A gradual low increase observed in the plasma high-density lipoprotein cholesterol of calves allows us to conclude that the increase of this lipid fraction after the supply of an additional lactose is associated with elevated expression of plasma apoA-IV.

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“…Total cellular RNA was isolated from liver tissue using a guanidinium thiocyanate/cesium chloride ultracentrifugation method as described by Sato et al (27). Complementary DNA was synthesized from 1.0 mg total RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche, Berlin, Germany).…”

Section: Methodsmentioning

confidence: 99%

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

Erami

Tanaka

Kawamura

et al. 2016

J Nutr Sci Vitaminol

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Summary Egg yolk is an important source of nutrients and contains different bioactive substances. In the present study, we studied the benefits of egg yolk in preventing low-protein-diet-induced fatty liver in rats. Rats were fed the following diets, which were based on the AIN-76 formula, for 2 wk: an adequate-protein diet containing 20% casein (C), a lowprotein diet containing 5% casein (LP-C), a low-protein diet supplemented with 12.5% egg yolk (LP-EY), and a low-protein diet supplemented with 4.1% egg yolk oil (LP-EYO). The lowprotein diets were adjusted to contain 4.13% protein and 4.7% lipids. The LP-C diet resulted in a greater increase in the liver trigriceride (TG) and the vacuolation and a greater decrease in the serum TG and free fatty acid (FFA) than did the C diet. These deviations in the serum and liver TG, serum FFA levels and the liver histopathology were corrected in rats fed the LP-EY diet but not in those fed the LP-EYO diet. Compared to rats fed the LP-C diet, although the activities of lipogenesis-related enzymes (fatty acid synthase, glucose-6-phosphate dehydrogenase, and malic enzyme) decreased in rats fed both of the LP-EY and LP-EYO diets, the level of the microsomal TG transfer protein (MTP) increased only in rats fed the LP-EY diet. Collectively, these results suggest that dietary egg yolk supplementation decreases the LP diet-induced accumulation of TG in the liver by increasing transport of TG in the liver, and egg yolk oil alone is not sufficient enough to bring about these benefits.

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Regulation of intestinal apo A-IV mRNA abundance in rat pups during fasting and refeeding

Cited by 19 publications

References 33 publications

Dietary Soy Protein Isolate and Its Undigested High Molecular Fraction Upregulate Hepatic ATP-Binding Cassette Transporter G5 and ATP-Binding Cassette Transporter G8 mRNA and Increase Biliary Secretion of Cholesterol in Rats

Dietary Soy Protein Isolate and Its Undigested High Molecular Fraction Upregulate Hepatic ATP-Binding Cassette Transporter G5 and ATP-Binding Cassette Transporter G8 mRNA and Increase Biliary Secretion of Cholesterol in Rats

Analysis of apolipoprotein A-IV expression and the levels of blood plasmamain lipid fractions of calves during the first week of life before and after theadministration of milk replacer supplemented with lactose

Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats

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