Regulation of interleukin-1 receptors and hypothalamic-pituitary-adrenal axis by lipopolysaccharide treatment in the mouse

Takao, Toshihiro; Nakata, Hideshi; Tojo, Chisa; Kurokawa, Hisayo; Nishioka, Tatsuya; Hashimoto, Kozo; Souza, Errol B. De

doi:10.1016/0006-8993(94)91073-1

Cited by 22 publications

(9 citation statements)

References 30 publications

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“…The studies carried out on mice [34] and sheep [31] also reported the stimulating effect of LPS on IL-1R1 mRNA. However, the other studies suggested the inhibitory effect of LPS on IL-1R1 gene expression in the pituitary [35, 36], In the present study observed elevation of sheep IL-1R1 mRNA does not have to be an effect of direct action of LPS on pituitary cells. It could be caused by stress of inflammatory response.…”

Section: Discussioncontrasting

confidence: 55%

LPS-Induced Inflammation Potentiates the IL-1-Mediated Reduction of LH Secretion from the Anterior Pituitary Explants

Herman

Krawczyńska

Bochenek

et al. 2013

Clinical and Developmental Immunology

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Acting at the level of the brain, interleukin- (IL-)1β is considered to be one of the most potent downregulators of reproduction processes during immune/inflammatory challenge. IL-1β suppresses gonadotropin-releasing hormone (GnRH) secretion from the hypothalamus resulting in the inhibition of the luteinizing hormone (LH) release from the anterior pituitary (AP). However, the presence of IL-1β receptors in the AP suggests the possible direct action of this cytokine on LH secretion. The study was designed to determine the effect of IL-1β on the LH secretion from the AP explants collected from saline and LPS-treated ewes in the follicular phase. It was found that IL-1β suppressed (P ≤ 0.01) GnRH-stimulated LH release and LHβ gene expression in AP explants in both groups. However, IL-1β action was more potent in the explants collected from LPS-treated animals. Pituitaries from LPS-treated animals were characterized by increased (P ≤ 0.01) IL-1 type I receptor and decreased (P ≤ 0.01) GnRH receptor gene expression level compared to the saline-treated group. IL-1β also affected the GnRH-R gene expression in explants collected from LPS-treated animals. Our results show that direct action of IL-1β on the pituitary gonadotropes could be one of the reasons of the reproductive processes disorders accompanying an inflammatory state.

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Section: Discussioncontrasting

confidence: 55%

LPS-Induced Inflammation Potentiates the IL-1-Mediated Reduction of LH Secretion from the Anterior Pituitary Explants

Herman

Krawczyńska

Bochenek

et al. 2013

Clinical and Developmental Immunology

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“…Although the receptors for IL-l jS and IL-6 have also been demonstrated in the rodent anterior pituitary [41][42][43][44][45], our present data strongly suggest that the direct action of the cytokines on the pituitary may not be important in inducing the early, peak phase of ACTH response to LPS. It has been repeatedly shown that LPS itself is without effect on CRH [46,47] and arginine vasopressin [47] release from rat hypothalamic fragments in vitro.…”

Section: Discussioncontrasting

confidence: 50%

Temporal Profiles of Interleukin-1.BETA., Interleukin-6, and Tumor Necrosis Factor-.ALPHA. in the Plasma and Hypothalamic Paraventricular Nucleus after Intravenous or Intraperitoneal Administration of Lipopolysaccharide in the Rat. Estimation by Push-Pull Perfusion.

et al. 1999

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“…The increment of IL-1 receptors by LPS was further supported utilizing a [ 125 I]IL-1ß binding assay. We and others have previously shown that IL-1 binding was decreased after LPS treatment in various murine brain and peripheral tissues [9,[13][14][15]. The present results clearly demonstrate that the decrease of IL-1 receptors in vivo is not due to a direct effect of LPS since IL-1 receptors are increased in AtT-20 cells in vitro while available receptors in vivo are decreased followed by LPS treatment.…”

Section: Discussionsupporting

confidence: 70%

“…We and others have previously shown that IL-1 binding was decreased after LPS treatment in the mouse in the various brain and peripheral tissues [9,[13][14][15]. It has recently been reported that a single injection of LPS increases type I IL-1 receptor (IL-1RI) mRNA levels in the whole brain, cerebral cortex, spleen [16] and liver [17].…”

Section: Introductionmentioning

confidence: 99%

Modulation of Interleukin-1 Receptors Followed by Endotoxin Lipopolysaccharide Treatment in the Mouse AtT-20 Pituitary Tumor Cell Line

Kumagai¹,

Takao²,

Matsumoto³

et al. 2002

Neuroimmunomodulation

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Objective: We have previously reported the characterization and regulation of interleukin-1 (IL-1) receptors utilizing [¹²⁵I]IL-1 binding assay in male C57BL/6 mice and the mouse AtT-20 pituitary tumor cells. In the present study, we examine IL-1 receptors using an immunoblotting method to further characterize the mechanisms regulating the interactions of IL-1 receptors with endotoxin, lipopolysaccharide (LPS). Methods: We established Western blotting for IL-1 receptors using AtT-20 mouse pituitary tumor cells. Results: Several bands were seen; however, only the 105-kD band was neutralized with a 5-fold excess of IL-1 receptor- blocking peptides, suggesting that this band is specific for IL-1 receptors. Next, we investigated the effect of LPS and IL-1β on IL-1 receptors. Treatment of AtT-20 cells with 0.01 µg/ml of LPS did not affect IL-1 receptors. In contrast, 1 µg/ml of LPS significantly increased IL-1 receptors in AtT-20 cells compared with the control group. In addition, [¹²⁵I]IL-1β binding was markedly increased followed by 1 µg/ml of LPS. In contrast, 1 nM recombinant human IL-1β significantly decreased IL-1 receptors in AtT-20 cells compared with the control group although treatment of AtT-20 cells with 0.01 nM IL-1β did not affect IL-1 receptors. LPS (0.1 and 1 µg/ml) did not affect IL-1β concentrations in the medium of AtT-20 cell culture. IL-1β concentrations in the homogenates from AtT-20 cells were significantly decreased after 1 µg/ml of LPS treatment but not after 0.01 µg/ml LPS. Conclusions: These data demonstrate that LPS and IL-1β differentially modulate IL-1 receptors in AtT-20 cells and LPS-induced modulation of IL-1 receptors may provide a novel mechanism for the actions of LPS to alter pituitary function during endotoxemia. Additional in vivo studies are necessary to determine the physiological relevance of this in vitro phenomenon.

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Regulation of interleukin-1 receptors and hypothalamic-pituitary-adrenal axis by lipopolysaccharide treatment in the mouse

Cited by 22 publications

References 30 publications

LPS-Induced Inflammation Potentiates the IL-1-Mediated Reduction of LH Secretion from the Anterior Pituitary Explants

LPS-Induced Inflammation Potentiates the IL-1-Mediated Reduction of LH Secretion from the Anterior Pituitary Explants

Temporal Profiles of Interleukin-1.BETA., Interleukin-6, and Tumor Necrosis Factor-.ALPHA. in the Plasma and Hypothalamic Paraventricular Nucleus after Intravenous or Intraperitoneal Administration of Lipopolysaccharide in the Rat. Estimation by Push-Pull Perfusion.

Modulation of Interleukin-1 Receptors Followed by Endotoxin Lipopolysaccharide Treatment in the Mouse AtT-20 Pituitary Tumor Cell Line

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