Regulation of Inhibitory and Activating Killer-Cell Ig-Like Receptor Expression Occurs in T Cells After Termination of TCR Rearrangements

Vély, Frederic; Peyrat, Marie-Alix; Couedel, Christelle; Morcet, Jean‐François; Halary, Franck; Davodeau, François; Romagné, François; Scotet, Emmanuel; Saulquin, Xavier; Houssaint, Elisabeth; Schleinitz, N.; Moretta, Alessandro; Vivier, Éric; Bonneville, Marc

doi:10.4049/jimmunol.166.4.2487

Cited by 76 publications

(60 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This concerned both the lectin-and Ig-like MHC-NKR, even though the first was quantitatively more expressed than the second, as previously observed for polyclonal peripheral blood cd T lymphocytes [24]. This observation lends further support to the conclusions deduced from the results previously obtained on clones generated in vitro [9][10][11], because it has the advantage to be done on freshly isolated PBMC. Accordingly, we noted here that the Ig-and lectin-like MHC-NKR phenotype of in vitro derived T cell clones did not strictly correspond to that of the fresh PBMC.…”

Section: Discussionsupporting

confidence: 88%

“…Some reports suggested that in vivo MHC-NKR expression might follow the law of "all or none", at least in T cell leukemias [7,8], whereas others revealed that several anti-EBV [9], anti-tumoral [10] or donor peripheral blood [11] CD8 + ab T cell clones can display different patterns of Ig-like or lectin-like MHC-NKR even when they express identical TCR. Taken altogether, these results sustain the notion that MHC-NKR expression occurs after TCR rearrangement.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of MHC class I receptors confers functional intraclonal heterogeneity to a reactive expansion of γδ T cells

et al. 2005

Self Cite

View full text Add to dashboard Cite

NK cell receptors for MHC class I molecules (MHC-NKR) can be expressed by T cell subsets. The restricted repertoire and phenotypic characteristics of MHC-NKR + T cells indicate that expression of MHC-NKR is acquired upon antigenic challenge and might promote expansion of T cells. Previous studies performed on in vitro generated ab T cell clones concluded that MHC-NKR expression was not a clonal attribute. Here, we examined a massive monoclonal expansion of a non-leukemic cd T cell population found in the peripheral blood of a lung-transplanted patient who suffered from a cytomegalovirus infection. Despite their monoclonality, these T cells displayed a heterogeneous and stable in vivo Ig-and lectin-like MHC-NKR phenotype. Twenty percent of the cells displayed a CD94 + NKG2A + phenotype, and 10% were labeled with an anti-CD158b1/b2/j monoclonal antibody. A CD158b/j + cd T cell clone derived in vitro from patient's peripheral blood lymphocytes was shown to express the activating form CD158j (KIR2DS2), which once cross-linked stimulated the clone cytolytic function and costimulated the TCR-induced production of cytokines, independently of the killer-activating receptor-associated protein (KARAP). In conclusion, heterogeneity of MHC-NKR expression confers a functional intraclonal diversity that may participate to induction of specific cd T cell effector functions or proliferation upon pathogen challenge.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Expression of MHC class I receptors confers functional intraclonal heterogeneity to a reactive expansion of γδ T cells

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…KIR ϩ CD8 T cells of healthy donors and KIR ϩ CD28 Ϫ CD4 T cells from rheumatoid arthritis patients are polyclonal, but do have a restricted TCR repertoire (13,38,39). The ten KIR ϩ clones examined carried at least five different TCR (Fig.…”

Section: Tcr Diversity Of Kir ϩ Cd4 T Cellsmentioning

confidence: 99%

Phenotypic and Functional Characterization of CD4 T Cells Expressing Killer Ig-Like Receptors

Bergen

Thompson

Slik

et al. 2004

The Journal of Immunology

110

View full text Add to dashboard Cite

Killer Ig-like receptors (KIR) are commonly found on human NK cells, γδ T cells, and CD8 T cells. Although KIR+ CD4 T cells are found in certain patients, their prevalence in healthy donors is controversial. We now provide definitive proof that such cells are present in most individuals, and report on their frequency, surface phenotype, cytokine profile, and Ag specificity. The number of KIR+ CD4 T cells detected in peripheral blood increased with age. In contrast with regular KIR− CD4 T cells, the majority of KIR+ CD4 T cells lacked surface expression of CD27, CD28, CCR4, and CCR7, but did express CD57 and 2B4. In addition, KIR were detected on approximately one-tenth of CD28− and CD57+ memory CD4 T cells. In line with the absence of the Th2 marker CCR4, the KIR+ CD4 cells produced mainly IFN-γ and little IL-4, IL-10, or IL-17 upon TCR triggering. Furthermore, the KIR+ population contained cells that responded to recall Ags in an HLA class II-restricted fashion. Together, our data indicate that KIR-expressing CD4 T cells are predominantly HLA class II-restricted effector memory Th1 cells, and that a significant, previously unrecognized fraction of effector memory Th1 cells expresses KIR.

show abstract

“…CD8 memory T cells, some CD4 T cells (22), and a population of ␥␦ T cells also express KIR with a similar expression pattern as that observed on NK cells. Diversification of the KIR repertoire occurs following clonal expansion of naive T cells, suggesting that populations of expanded T cell clones are diverse in their capacity to be stimulated, according to their KIR repertoire (23,24).…”

mentioning

confidence: 99%

“…KIR3DL3 (otherwise known as KIR3DL7 or KIRC1) does not appear to be expressed by the circulating NK cell pool in many donors (29). Most reports indicate that all NK clones and KIR-positive T cell clones express KIR2DL4 transcripts (16,23,24,30,31). The upstream regions of both these KIR genes diverge significantly from those of KIR with variegated expression (26).…”

mentioning

confidence: 99%

Different and Divergent Regulation of the KIR2DL4 and KIR3DL1 Promoters

Stewart

Bergen

Trowsdale

2003

The Journal of Immunology

View full text Add to dashboard Cite

The killer Ig-like receptors (KIR) are a family of highly related MHC class I receptors that show extreme genetic polymorphism both within the human population and between closely related primate species, suggestive of rapid evolutionary diversification. Most KIR are expressed in a variegated fashion by the NK population, giving rise to an NK repertoire of specificities for MHC class I. We compared the promoter for KIR3DL1, which exhibits variegated gene expression, with that for KIR2DL4, which is expressed by all NK cell clones. Maximum transcriptional activity of each was encoded within ∼270 bp upstream of the translation initiation codon. The KIR2DL4 promoter drove reporter gene expression only in NK cells, while the KIR3DL1 promoter was active in a range of cell types, suggesting that the latter requires other regulatory elements for physiological expression. In NK cells, reporter gene expression driven by the KIR2DL4 promoter was greater than that driven by the KIR3DL1 promoter. DNase I footprinting revealed that transcription factor binding sites differ between the two promoters. The data indicate that while the promoters of these two KIR genes share 67% nucleotide identity, they have evolved distinct properties consistent with different roles in regulating the generation of NK repertoire.

show abstract

Regulation of Inhibitory and Activating Killer-Cell Ig-Like Receptor Expression Occurs in T Cells After Termination of TCR Rearrangements

Cited by 76 publications

References 59 publications

Expression of MHC class I receptors confers functional intraclonal heterogeneity to a reactive expansion of γδ T cells

Expression of MHC class I receptors confers functional intraclonal heterogeneity to a reactive expansion of γδ T cells

Phenotypic and Functional Characterization of CD4 T Cells Expressing Killer Ig-Like Receptors

Different and Divergent Regulation of the KIR2DL4 and KIR3DL1 Promoters

Contact Info

Product

Resources

About